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The Role of Marek’s Disease Virus UL12 and UL29 in DNA Recombination and the Virus Lifecycle

Marek’s disease virus (MDV) is an oncogenic alphaherpesvirus that infects chickens and integrates its genome into the telomeres of latently infected cells. MDV encodes two proteins, UL12 and UL29 (ICP8), that are conserved among herpesviruses and could facilitate virus integration. The orthologues o...

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Autores principales: Previdelli, Renato L., Bertzbach, Luca D., Wight, Darren J., Vychodil, Tereza, You, Yu, Arndt, Sina, Kaufer, Benedikt B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409567/
https://www.ncbi.nlm.nih.gov/pubmed/30696089
http://dx.doi.org/10.3390/v11020111
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author Previdelli, Renato L.
Bertzbach, Luca D.
Wight, Darren J.
Vychodil, Tereza
You, Yu
Arndt, Sina
Kaufer, Benedikt B.
author_facet Previdelli, Renato L.
Bertzbach, Luca D.
Wight, Darren J.
Vychodil, Tereza
You, Yu
Arndt, Sina
Kaufer, Benedikt B.
author_sort Previdelli, Renato L.
collection PubMed
description Marek’s disease virus (MDV) is an oncogenic alphaherpesvirus that infects chickens and integrates its genome into the telomeres of latently infected cells. MDV encodes two proteins, UL12 and UL29 (ICP8), that are conserved among herpesviruses and could facilitate virus integration. The orthologues of UL12 and UL29 in herpes simplex virus 1 (HSV-1) possess exonuclease and single strand DNA-binding activity, respectively, and facilitate DNA recombination; however, the role of both proteins in the MDV lifecycle remains elusive. To determine if UL12 and/or UL29 are involved in virus replication, we abrogated their expression in the very virulent RB-1B strain. Abrogation of either UL12 or UL29 resulted in a severe impairment of virus replication. We also demonstrated that MDV UL12 can aid in single strand annealing DNA repair, using a well-established reporter cell line. Finally, we assessed the role of UL12 and UL29 in MDV integration and maintenance of the latent virus genome. We could demonstrate that knockdown of UL12 and UL29 does not interfere with the establishment or maintenance of latency. Our data therefore shed light on the role of MDV UL12 and UL29 in MDV replication, DNA repair, and maintenance of the latent virus genome.
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spelling pubmed-64095672019-04-01 The Role of Marek’s Disease Virus UL12 and UL29 in DNA Recombination and the Virus Lifecycle Previdelli, Renato L. Bertzbach, Luca D. Wight, Darren J. Vychodil, Tereza You, Yu Arndt, Sina Kaufer, Benedikt B. Viruses Article Marek’s disease virus (MDV) is an oncogenic alphaherpesvirus that infects chickens and integrates its genome into the telomeres of latently infected cells. MDV encodes two proteins, UL12 and UL29 (ICP8), that are conserved among herpesviruses and could facilitate virus integration. The orthologues of UL12 and UL29 in herpes simplex virus 1 (HSV-1) possess exonuclease and single strand DNA-binding activity, respectively, and facilitate DNA recombination; however, the role of both proteins in the MDV lifecycle remains elusive. To determine if UL12 and/or UL29 are involved in virus replication, we abrogated their expression in the very virulent RB-1B strain. Abrogation of either UL12 or UL29 resulted in a severe impairment of virus replication. We also demonstrated that MDV UL12 can aid in single strand annealing DNA repair, using a well-established reporter cell line. Finally, we assessed the role of UL12 and UL29 in MDV integration and maintenance of the latent virus genome. We could demonstrate that knockdown of UL12 and UL29 does not interfere with the establishment or maintenance of latency. Our data therefore shed light on the role of MDV UL12 and UL29 in MDV replication, DNA repair, and maintenance of the latent virus genome. MDPI 2019-01-28 /pmc/articles/PMC6409567/ /pubmed/30696089 http://dx.doi.org/10.3390/v11020111 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Previdelli, Renato L.
Bertzbach, Luca D.
Wight, Darren J.
Vychodil, Tereza
You, Yu
Arndt, Sina
Kaufer, Benedikt B.
The Role of Marek’s Disease Virus UL12 and UL29 in DNA Recombination and the Virus Lifecycle
title The Role of Marek’s Disease Virus UL12 and UL29 in DNA Recombination and the Virus Lifecycle
title_full The Role of Marek’s Disease Virus UL12 and UL29 in DNA Recombination and the Virus Lifecycle
title_fullStr The Role of Marek’s Disease Virus UL12 and UL29 in DNA Recombination and the Virus Lifecycle
title_full_unstemmed The Role of Marek’s Disease Virus UL12 and UL29 in DNA Recombination and the Virus Lifecycle
title_short The Role of Marek’s Disease Virus UL12 and UL29 in DNA Recombination and the Virus Lifecycle
title_sort role of marek’s disease virus ul12 and ul29 in dna recombination and the virus lifecycle
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409567/
https://www.ncbi.nlm.nih.gov/pubmed/30696089
http://dx.doi.org/10.3390/v11020111
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