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Inhibitory Effects of Sodium Alginate on Hepatic Steatosis in Mice Induced by a Methionine- and Choline-Deficient Diet

Nonalcoholic steatohepatitis (NASH) progresses from nonalcoholic fatty liver disease (NAFLD); however, efficacious drugs for NASH treatment are lacking. Sodium alginate (SA), a soluble dietary fiber extracted from brown algae, could protect the small intestine from enterobacterial invasion. NASH pat...

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Autores principales: Kawauchi, Shoji, Horibe, Sayo, Sasaki, Naoto, Tanahashi, Toshihito, Mizuno, Shigeto, Hamaguchi, Tsuneo, Rikitake, Yoshiyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409683/
https://www.ncbi.nlm.nih.gov/pubmed/30744124
http://dx.doi.org/10.3390/md17020104
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author Kawauchi, Shoji
Horibe, Sayo
Sasaki, Naoto
Tanahashi, Toshihito
Mizuno, Shigeto
Hamaguchi, Tsuneo
Rikitake, Yoshiyuki
author_facet Kawauchi, Shoji
Horibe, Sayo
Sasaki, Naoto
Tanahashi, Toshihito
Mizuno, Shigeto
Hamaguchi, Tsuneo
Rikitake, Yoshiyuki
author_sort Kawauchi, Shoji
collection PubMed
description Nonalcoholic steatohepatitis (NASH) progresses from nonalcoholic fatty liver disease (NAFLD); however, efficacious drugs for NASH treatment are lacking. Sodium alginate (SA), a soluble dietary fiber extracted from brown algae, could protect the small intestine from enterobacterial invasion. NASH pathogenesis has been suggested to be associated with enterobacterial invasion, so we examined the effect of SA on methionine- and choline-deficient (MCD) diet-induced steatohepatitis in mice (the most widely-used model of NASH). The mice (n = 31) were divided into three groups (mice fed with regular chow, MCD diet, and MCD diet premixed with 5% SA) for 4 and 8 weeks. The MCD diet increased lipid accumulation and inflammation in the liver, the NAFLD Activity Score and hepatic mRNA expression of tumor necrosis factor-α and collagen 1α1, and induced macrophage infiltration. Villus shortening, disruption of zonula occludens-1 localization and depletion of mucus production were observed in the small intestine of the MCD-group mice. SA administration improved lipid accumulation and inflammation in the liver, and impaired barrier function in the small intestine. Collectively, these results suggest that SA is useful for NASH treatment because it can prevent hepatic inflammation and fatty degeneration by maintaining intestinal barrier function.
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spelling pubmed-64096832019-03-29 Inhibitory Effects of Sodium Alginate on Hepatic Steatosis in Mice Induced by a Methionine- and Choline-Deficient Diet Kawauchi, Shoji Horibe, Sayo Sasaki, Naoto Tanahashi, Toshihito Mizuno, Shigeto Hamaguchi, Tsuneo Rikitake, Yoshiyuki Mar Drugs Article Nonalcoholic steatohepatitis (NASH) progresses from nonalcoholic fatty liver disease (NAFLD); however, efficacious drugs for NASH treatment are lacking. Sodium alginate (SA), a soluble dietary fiber extracted from brown algae, could protect the small intestine from enterobacterial invasion. NASH pathogenesis has been suggested to be associated with enterobacterial invasion, so we examined the effect of SA on methionine- and choline-deficient (MCD) diet-induced steatohepatitis in mice (the most widely-used model of NASH). The mice (n = 31) were divided into three groups (mice fed with regular chow, MCD diet, and MCD diet premixed with 5% SA) for 4 and 8 weeks. The MCD diet increased lipid accumulation and inflammation in the liver, the NAFLD Activity Score and hepatic mRNA expression of tumor necrosis factor-α and collagen 1α1, and induced macrophage infiltration. Villus shortening, disruption of zonula occludens-1 localization and depletion of mucus production were observed in the small intestine of the MCD-group mice. SA administration improved lipid accumulation and inflammation in the liver, and impaired barrier function in the small intestine. Collectively, these results suggest that SA is useful for NASH treatment because it can prevent hepatic inflammation and fatty degeneration by maintaining intestinal barrier function. MDPI 2019-02-09 /pmc/articles/PMC6409683/ /pubmed/30744124 http://dx.doi.org/10.3390/md17020104 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kawauchi, Shoji
Horibe, Sayo
Sasaki, Naoto
Tanahashi, Toshihito
Mizuno, Shigeto
Hamaguchi, Tsuneo
Rikitake, Yoshiyuki
Inhibitory Effects of Sodium Alginate on Hepatic Steatosis in Mice Induced by a Methionine- and Choline-Deficient Diet
title Inhibitory Effects of Sodium Alginate on Hepatic Steatosis in Mice Induced by a Methionine- and Choline-Deficient Diet
title_full Inhibitory Effects of Sodium Alginate on Hepatic Steatosis in Mice Induced by a Methionine- and Choline-Deficient Diet
title_fullStr Inhibitory Effects of Sodium Alginate on Hepatic Steatosis in Mice Induced by a Methionine- and Choline-Deficient Diet
title_full_unstemmed Inhibitory Effects of Sodium Alginate on Hepatic Steatosis in Mice Induced by a Methionine- and Choline-Deficient Diet
title_short Inhibitory Effects of Sodium Alginate on Hepatic Steatosis in Mice Induced by a Methionine- and Choline-Deficient Diet
title_sort inhibitory effects of sodium alginate on hepatic steatosis in mice induced by a methionine- and choline-deficient diet
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409683/
https://www.ncbi.nlm.nih.gov/pubmed/30744124
http://dx.doi.org/10.3390/md17020104
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