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Testing the Neuroprotective Properties of PCSO-524(®) Using a Neuronal Cell Cycle Suppression Assay
Cell cycle reentry is a unified mechanism shared by several neurodegenerative diseases, including Alzheimer’s disease (AD) and Ataxia Telangiectasia (A-T). This phenotype is often related to neuroinflammation in the central nervous system. To mimic brain inflammation in vitro, we adopted the previou...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409808/ https://www.ncbi.nlm.nih.gov/pubmed/30682813 http://dx.doi.org/10.3390/md17020079 |
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author | Zhu, Beika Zhang, Yang Herrup, Karl |
author_facet | Zhu, Beika Zhang, Yang Herrup, Karl |
author_sort | Zhu, Beika |
collection | PubMed |
description | Cell cycle reentry is a unified mechanism shared by several neurodegenerative diseases, including Alzheimer’s disease (AD) and Ataxia Telangiectasia (A-T). This phenotype is often related to neuroinflammation in the central nervous system. To mimic brain inflammation in vitro, we adopted the previously established method of using conditioned medium collected from activated THP-1 cells and applied it to both differentiated HT22 cells and primary neurons. Unscheduled cell cycle events were observed in both systems, indicating the potential of this approach as an in vitro model of neurodegenerative disease. We used this assay to measure the neuroprotective effects of New Zealand green-lipped mussel extract, PCSO-524(®), to protect post-mitotic cells from cell cycle reentry. We found that, both in vitro and in an animal model, PCSO-524(®) displayed promising neuroprotective effects, and thus has potential to postpone or prevent the onset of neurodegenerative disease. |
format | Online Article Text |
id | pubmed-6409808 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64098082019-03-29 Testing the Neuroprotective Properties of PCSO-524(®) Using a Neuronal Cell Cycle Suppression Assay Zhu, Beika Zhang, Yang Herrup, Karl Mar Drugs Article Cell cycle reentry is a unified mechanism shared by several neurodegenerative diseases, including Alzheimer’s disease (AD) and Ataxia Telangiectasia (A-T). This phenotype is often related to neuroinflammation in the central nervous system. To mimic brain inflammation in vitro, we adopted the previously established method of using conditioned medium collected from activated THP-1 cells and applied it to both differentiated HT22 cells and primary neurons. Unscheduled cell cycle events were observed in both systems, indicating the potential of this approach as an in vitro model of neurodegenerative disease. We used this assay to measure the neuroprotective effects of New Zealand green-lipped mussel extract, PCSO-524(®), to protect post-mitotic cells from cell cycle reentry. We found that, both in vitro and in an animal model, PCSO-524(®) displayed promising neuroprotective effects, and thus has potential to postpone or prevent the onset of neurodegenerative disease. MDPI 2019-01-24 /pmc/articles/PMC6409808/ /pubmed/30682813 http://dx.doi.org/10.3390/md17020079 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhu, Beika Zhang, Yang Herrup, Karl Testing the Neuroprotective Properties of PCSO-524(®) Using a Neuronal Cell Cycle Suppression Assay |
title | Testing the Neuroprotective Properties of PCSO-524(®) Using a Neuronal Cell Cycle Suppression Assay |
title_full | Testing the Neuroprotective Properties of PCSO-524(®) Using a Neuronal Cell Cycle Suppression Assay |
title_fullStr | Testing the Neuroprotective Properties of PCSO-524(®) Using a Neuronal Cell Cycle Suppression Assay |
title_full_unstemmed | Testing the Neuroprotective Properties of PCSO-524(®) Using a Neuronal Cell Cycle Suppression Assay |
title_short | Testing the Neuroprotective Properties of PCSO-524(®) Using a Neuronal Cell Cycle Suppression Assay |
title_sort | testing the neuroprotective properties of pcso-524(®) using a neuronal cell cycle suppression assay |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409808/ https://www.ncbi.nlm.nih.gov/pubmed/30682813 http://dx.doi.org/10.3390/md17020079 |
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