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The Effect of DNA Topology on Observed Rates of R-Loop Formation and DNA Strand Cleavage by CRISPR Cas12a

Here we explored the mechanism of R-loop formation and DNA cleavage by type V CRISPR Cas12a (formerly known as Cpf1). We first used a single-molecule magnetic tweezers (MT) assay to show that R-loop formation by Lachnospiraceae bacterium ND2006 Cas12a is significantly enhanced by negative DNA superc...

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Detalles Bibliográficos
Autores principales:	van Aelst, Kara, Martínez-Santiago, Carlos J., Cross, Stephen J., Szczelkun, Mark D.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409811/ https://www.ncbi.nlm.nih.gov/pubmed/30813348 http://dx.doi.org/10.3390/genes10020169

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