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Identification of Genes Involved in Low Temperature-Induced Senescence of Panicle Leaf in Litchi chinensis
Warm winters and hot springs may promote panicle leaf growing and repress floral development. To identify genes potentially involved in litchi panicle leaf senescence, eight RNA-sequencing (RNA-Seq) libraries of the senescing panicle leaves under low temperature (LT) conditions and the developing pa...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409916/ https://www.ncbi.nlm.nih.gov/pubmed/30717231 http://dx.doi.org/10.3390/genes10020111 |
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author | Wang, Congcong Liu, Hao Yu, Sheng Chen, Houbin Hu, Fuchu Zhan, Huiling Pan, Xifen Lao, Yuhua Zhong, Silin Zhou, Biyan |
author_facet | Wang, Congcong Liu, Hao Yu, Sheng Chen, Houbin Hu, Fuchu Zhan, Huiling Pan, Xifen Lao, Yuhua Zhong, Silin Zhou, Biyan |
author_sort | Wang, Congcong |
collection | PubMed |
description | Warm winters and hot springs may promote panicle leaf growing and repress floral development. To identify genes potentially involved in litchi panicle leaf senescence, eight RNA-sequencing (RNA-Seq) libraries of the senescing panicle leaves under low temperature (LT) conditions and the developing panicle leaves under high temperature (HT) conditions were constructed. For each library, 4.78–8.99 × 10(6) clean reads were generated. Digital expression of the genes was compared between the senescing and developing panicle leaves. A total of 6477 upregulated differentially expressed genes (DEGs) (from developing leaves to senescing leaves), and 6318 downregulated DEGs were identified, 158 abscisic acid (ABA)-, 68 ethylene-, 107 indole-3-acetic acid (IAA)-, 27 gibberellic acid (GA)-, 68 cytokinin (CTK)-, 37 salicylic acid (SA)-, and 23 brassinolide (BR)-related DEGs. Confirmation of the RNA-Seq data by quantitative real-time PCR (qRT-PCR) analysis suggested that expression trends of the 10 candidate genes using qRT-PCR were similar to those revealed by RNA-Seq, and a significantly positive correlation between the obtained data from qRT-PCR and RNA-Seq were found, indicating the reliability of our RNA-Seq data. The present studies provided potential genes for the future molecular breeding of new cultivars that can induce panicle leaf senescence and reduce floral abortion under warm climates. |
format | Online Article Text |
id | pubmed-6409916 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64099162019-03-26 Identification of Genes Involved in Low Temperature-Induced Senescence of Panicle Leaf in Litchi chinensis Wang, Congcong Liu, Hao Yu, Sheng Chen, Houbin Hu, Fuchu Zhan, Huiling Pan, Xifen Lao, Yuhua Zhong, Silin Zhou, Biyan Genes (Basel) Article Warm winters and hot springs may promote panicle leaf growing and repress floral development. To identify genes potentially involved in litchi panicle leaf senescence, eight RNA-sequencing (RNA-Seq) libraries of the senescing panicle leaves under low temperature (LT) conditions and the developing panicle leaves under high temperature (HT) conditions were constructed. For each library, 4.78–8.99 × 10(6) clean reads were generated. Digital expression of the genes was compared between the senescing and developing panicle leaves. A total of 6477 upregulated differentially expressed genes (DEGs) (from developing leaves to senescing leaves), and 6318 downregulated DEGs were identified, 158 abscisic acid (ABA)-, 68 ethylene-, 107 indole-3-acetic acid (IAA)-, 27 gibberellic acid (GA)-, 68 cytokinin (CTK)-, 37 salicylic acid (SA)-, and 23 brassinolide (BR)-related DEGs. Confirmation of the RNA-Seq data by quantitative real-time PCR (qRT-PCR) analysis suggested that expression trends of the 10 candidate genes using qRT-PCR were similar to those revealed by RNA-Seq, and a significantly positive correlation between the obtained data from qRT-PCR and RNA-Seq were found, indicating the reliability of our RNA-Seq data. The present studies provided potential genes for the future molecular breeding of new cultivars that can induce panicle leaf senescence and reduce floral abortion under warm climates. MDPI 2019-02-01 /pmc/articles/PMC6409916/ /pubmed/30717231 http://dx.doi.org/10.3390/genes10020111 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Congcong Liu, Hao Yu, Sheng Chen, Houbin Hu, Fuchu Zhan, Huiling Pan, Xifen Lao, Yuhua Zhong, Silin Zhou, Biyan Identification of Genes Involved in Low Temperature-Induced Senescence of Panicle Leaf in Litchi chinensis |
title | Identification of Genes Involved in Low Temperature-Induced Senescence of Panicle Leaf in Litchi chinensis |
title_full | Identification of Genes Involved in Low Temperature-Induced Senescence of Panicle Leaf in Litchi chinensis |
title_fullStr | Identification of Genes Involved in Low Temperature-Induced Senescence of Panicle Leaf in Litchi chinensis |
title_full_unstemmed | Identification of Genes Involved in Low Temperature-Induced Senescence of Panicle Leaf in Litchi chinensis |
title_short | Identification of Genes Involved in Low Temperature-Induced Senescence of Panicle Leaf in Litchi chinensis |
title_sort | identification of genes involved in low temperature-induced senescence of panicle leaf in litchi chinensis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409916/ https://www.ncbi.nlm.nih.gov/pubmed/30717231 http://dx.doi.org/10.3390/genes10020111 |
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