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An In Vitro Investigation of Cytotoxic Effects of InP/Zns Quantum Dots with Different Surface Chemistries

Indium phosphide quantum dots (QDs) passivated with zinc sulphide in a core/shell architecture (InP/ZnS) with different surface chemistries were introduced to RAW 264.7 murine “macrophage-like” cells to understand their potential toxicities. The InP/ZnS quantum dots were conjugated with an oligonucl...

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Autores principales: Ayupova, Deanna, Dobhal, Garima, Laufersky, Geoffry, Nann, Thomas, Goreham, Renee V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409980/
https://www.ncbi.nlm.nih.gov/pubmed/30678192
http://dx.doi.org/10.3390/nano9020135
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author Ayupova, Deanna
Dobhal, Garima
Laufersky, Geoffry
Nann, Thomas
Goreham, Renee V.
author_facet Ayupova, Deanna
Dobhal, Garima
Laufersky, Geoffry
Nann, Thomas
Goreham, Renee V.
author_sort Ayupova, Deanna
collection PubMed
description Indium phosphide quantum dots (QDs) passivated with zinc sulphide in a core/shell architecture (InP/ZnS) with different surface chemistries were introduced to RAW 264.7 murine “macrophage-like” cells to understand their potential toxicities. The InP/ZnS quantum dots were conjugated with an oligonucleotide, a carboxylic acid, or an amino-polyethylene glycol ligand, and cell viability and cell proliferation were investigated via a metabolic assay. Membrane integrity was measured through the production of lactate dehydrogenase. Fluorescence microscopy showed cellular uptake. All quantum dots exhibited cytotoxic behaviour less than that observed from cadmium- or lead-based quantum dots; however, this behaviour was sensitive to the ligands used. In particular, the amino-polyethylene glycol conjugated quantum dots proved to possess the highest cytotoxicity examined here. This provides quantitative evidence that aqueous InP/ZnS quantum dots can offer a safer alternative for bioimaging or in therapeutic applications.
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spelling pubmed-64099802019-03-11 An In Vitro Investigation of Cytotoxic Effects of InP/Zns Quantum Dots with Different Surface Chemistries Ayupova, Deanna Dobhal, Garima Laufersky, Geoffry Nann, Thomas Goreham, Renee V. Nanomaterials (Basel) Article Indium phosphide quantum dots (QDs) passivated with zinc sulphide in a core/shell architecture (InP/ZnS) with different surface chemistries were introduced to RAW 264.7 murine “macrophage-like” cells to understand their potential toxicities. The InP/ZnS quantum dots were conjugated with an oligonucleotide, a carboxylic acid, or an amino-polyethylene glycol ligand, and cell viability and cell proliferation were investigated via a metabolic assay. Membrane integrity was measured through the production of lactate dehydrogenase. Fluorescence microscopy showed cellular uptake. All quantum dots exhibited cytotoxic behaviour less than that observed from cadmium- or lead-based quantum dots; however, this behaviour was sensitive to the ligands used. In particular, the amino-polyethylene glycol conjugated quantum dots proved to possess the highest cytotoxicity examined here. This provides quantitative evidence that aqueous InP/ZnS quantum dots can offer a safer alternative for bioimaging or in therapeutic applications. MDPI 2019-01-22 /pmc/articles/PMC6409980/ /pubmed/30678192 http://dx.doi.org/10.3390/nano9020135 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ayupova, Deanna
Dobhal, Garima
Laufersky, Geoffry
Nann, Thomas
Goreham, Renee V.
An In Vitro Investigation of Cytotoxic Effects of InP/Zns Quantum Dots with Different Surface Chemistries
title An In Vitro Investigation of Cytotoxic Effects of InP/Zns Quantum Dots with Different Surface Chemistries
title_full An In Vitro Investigation of Cytotoxic Effects of InP/Zns Quantum Dots with Different Surface Chemistries
title_fullStr An In Vitro Investigation of Cytotoxic Effects of InP/Zns Quantum Dots with Different Surface Chemistries
title_full_unstemmed An In Vitro Investigation of Cytotoxic Effects of InP/Zns Quantum Dots with Different Surface Chemistries
title_short An In Vitro Investigation of Cytotoxic Effects of InP/Zns Quantum Dots with Different Surface Chemistries
title_sort in vitro investigation of cytotoxic effects of inp/zns quantum dots with different surface chemistries
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409980/
https://www.ncbi.nlm.nih.gov/pubmed/30678192
http://dx.doi.org/10.3390/nano9020135
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