Cargando…

Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs

In the frog genus Pseudis, previous works found a sex-linked heteromorphism of the PcP190 satellite DNA in the nucleolus organizer region (NOR)-bearing chromosome pairs of Pseudis bolbodactyla and Pseudis tocantins, which possess a ZZ/ZW sex determination system. A pericentromeric inversion was infe...

Descripción completa

Detalles Bibliográficos
Autores principales: Gatto, Kaleb Pretto, Seger, Karin Regina, Garcia, Paulo Christiano de Anchieta, Lourenço, Luciana Bolsoni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410007/
https://www.ncbi.nlm.nih.gov/pubmed/30791490
http://dx.doi.org/10.3390/genes10020160
_version_ 1783402129927438336
author Gatto, Kaleb Pretto
Seger, Karin Regina
Garcia, Paulo Christiano de Anchieta
Lourenço, Luciana Bolsoni
author_facet Gatto, Kaleb Pretto
Seger, Karin Regina
Garcia, Paulo Christiano de Anchieta
Lourenço, Luciana Bolsoni
author_sort Gatto, Kaleb Pretto
collection PubMed
description In the frog genus Pseudis, previous works found a sex-linked heteromorphism of the PcP190 satellite DNA in the nucleolus organizer region (NOR)-bearing chromosome pairs of Pseudis bolbodactyla and Pseudis tocantins, which possess a ZZ/ZW sex determination system. A pericentromeric inversion was inferred to have occurred during W chromosome evolution, moving a chromosomal cluster enriched by the PcP190 from the short arm (as observed in P. bolbodactyla) to the NOR-bearing long arm (as observed in P. tocantins). However, whether such an inversion happened in P. tocantins or in the common ancestor of Pseudis fusca and P. tocantins remained unclear. To assess this question, we mapped PcP190 in the karyotype of P. fusca from three distinct localities. Southern blotting was used to compare males and females. The mitochondrial H1 fragment (which contains the 12S ribosomal RNA (rRNA), tRNAval, and 16S rRNA genes) and cytochrome b gene were partially sequenced, and a species tree was inferred to guide our analysis. Pseudis fusca specimens were placed together as the sister group of P. tocantins, but based on genetic distance, one of the analyzed populations is probably an undescribed species. A cluster of PcP190, located in the long arm of chromosome 7, is sex linked in this putative new species but not in the remaining P. fusca. We could infer that the pericentromeric inversion that moved the PcP190 site to the NOR-bearing chromosome arm (long arm) occurred in the common ancestor of P. fusca, the putative undescribed species, and P. tocantins.
format Online
Article
Text
id pubmed-6410007
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-64100072019-03-26 Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs Gatto, Kaleb Pretto Seger, Karin Regina Garcia, Paulo Christiano de Anchieta Lourenço, Luciana Bolsoni Genes (Basel) Article In the frog genus Pseudis, previous works found a sex-linked heteromorphism of the PcP190 satellite DNA in the nucleolus organizer region (NOR)-bearing chromosome pairs of Pseudis bolbodactyla and Pseudis tocantins, which possess a ZZ/ZW sex determination system. A pericentromeric inversion was inferred to have occurred during W chromosome evolution, moving a chromosomal cluster enriched by the PcP190 from the short arm (as observed in P. bolbodactyla) to the NOR-bearing long arm (as observed in P. tocantins). However, whether such an inversion happened in P. tocantins or in the common ancestor of Pseudis fusca and P. tocantins remained unclear. To assess this question, we mapped PcP190 in the karyotype of P. fusca from three distinct localities. Southern blotting was used to compare males and females. The mitochondrial H1 fragment (which contains the 12S ribosomal RNA (rRNA), tRNAval, and 16S rRNA genes) and cytochrome b gene were partially sequenced, and a species tree was inferred to guide our analysis. Pseudis fusca specimens were placed together as the sister group of P. tocantins, but based on genetic distance, one of the analyzed populations is probably an undescribed species. A cluster of PcP190, located in the long arm of chromosome 7, is sex linked in this putative new species but not in the remaining P. fusca. We could infer that the pericentromeric inversion that moved the PcP190 site to the NOR-bearing chromosome arm (long arm) occurred in the common ancestor of P. fusca, the putative undescribed species, and P. tocantins. MDPI 2019-02-19 /pmc/articles/PMC6410007/ /pubmed/30791490 http://dx.doi.org/10.3390/genes10020160 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gatto, Kaleb Pretto
Seger, Karin Regina
Garcia, Paulo Christiano de Anchieta
Lourenço, Luciana Bolsoni
Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs
title Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs
title_full Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs
title_fullStr Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs
title_full_unstemmed Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs
title_short Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs
title_sort satellite dna mapping in pseudis fusca (hylidae, pseudinae) provides new insights into sex chromosome evolution in paradoxical frogs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410007/
https://www.ncbi.nlm.nih.gov/pubmed/30791490
http://dx.doi.org/10.3390/genes10020160
work_keys_str_mv AT gattokalebpretto satellitednamappinginpseudisfuscahylidaepseudinaeprovidesnewinsightsintosexchromosomeevolutioninparadoxicalfrogs
AT segerkarinregina satellitednamappinginpseudisfuscahylidaepseudinaeprovidesnewinsightsintosexchromosomeevolutioninparadoxicalfrogs
AT garciapaulochristianodeanchieta satellitednamappinginpseudisfuscahylidaepseudinaeprovidesnewinsightsintosexchromosomeevolutioninparadoxicalfrogs
AT lourencolucianabolsoni satellitednamappinginpseudisfuscahylidaepseudinaeprovidesnewinsightsintosexchromosomeevolutioninparadoxicalfrogs