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Ebola Virus Isolation Using Huh-7 Cells has Methodological Advantages and Similar Sensitivity to Isolation Using Other Cell Types and Suckling BALB/c Laboratory Mice
Following the largest Ebola virus disease outbreak from 2013 to 2016, viral RNA has been detected in survivors from semen and breast milk long after disease recovery. However, as there have been few cases of sexual transmission, it is unclear whether every RNA positive fluid sample contains infectio...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410085/ https://www.ncbi.nlm.nih.gov/pubmed/30781518 http://dx.doi.org/10.3390/v11020161 |
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author | Logue, James Vargas Licona, Walter Cooper, Timothy K. Reeder, Becky Byrum, Russel Qin, Jing Deiuliis Murphy, Nicole Cong, Yu Bonilla, Amanda Sword, Jennifer Weaver, Wade Kocher, Gregory Olinger, Gene G. Jahrling, Peter B. Hensley, Lisa E. Bennett, Richard S. |
author_facet | Logue, James Vargas Licona, Walter Cooper, Timothy K. Reeder, Becky Byrum, Russel Qin, Jing Deiuliis Murphy, Nicole Cong, Yu Bonilla, Amanda Sword, Jennifer Weaver, Wade Kocher, Gregory Olinger, Gene G. Jahrling, Peter B. Hensley, Lisa E. Bennett, Richard S. |
author_sort | Logue, James |
collection | PubMed |
description | Following the largest Ebola virus disease outbreak from 2013 to 2016, viral RNA has been detected in survivors from semen and breast milk long after disease recovery. However, as there have been few cases of sexual transmission, it is unclear whether every RNA positive fluid sample contains infectious virus. Virus isolation, typically using cell culture or animal models, can serve as a tool to determine the infectivity of patient samples. However, the sensitivity of these methods has not been assessed for the Ebola virus isolate, Makona. Described here is an efficiency comparison of Ebola virus Makona isolation using Vero E6, Huh-7, monocyte-derived macrophage cells, and suckling laboratory mice. Isolation sensitivity was similar in all methods tested. Laboratory mice and Huh-7 cells were less affected by toxicity from breast milk than Vero E6 and MDM cells. However, the advantages associated with isolation in Huh-7 cells over laboratory mice, including cost effectiveness, sample volume preservation, and a reduction in animal use, make Huh-7 cells the preferred substrate tested for Ebola virus Makona isolation. |
format | Online Article Text |
id | pubmed-6410085 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64100852019-04-01 Ebola Virus Isolation Using Huh-7 Cells has Methodological Advantages and Similar Sensitivity to Isolation Using Other Cell Types and Suckling BALB/c Laboratory Mice Logue, James Vargas Licona, Walter Cooper, Timothy K. Reeder, Becky Byrum, Russel Qin, Jing Deiuliis Murphy, Nicole Cong, Yu Bonilla, Amanda Sword, Jennifer Weaver, Wade Kocher, Gregory Olinger, Gene G. Jahrling, Peter B. Hensley, Lisa E. Bennett, Richard S. Viruses Article Following the largest Ebola virus disease outbreak from 2013 to 2016, viral RNA has been detected in survivors from semen and breast milk long after disease recovery. However, as there have been few cases of sexual transmission, it is unclear whether every RNA positive fluid sample contains infectious virus. Virus isolation, typically using cell culture or animal models, can serve as a tool to determine the infectivity of patient samples. However, the sensitivity of these methods has not been assessed for the Ebola virus isolate, Makona. Described here is an efficiency comparison of Ebola virus Makona isolation using Vero E6, Huh-7, monocyte-derived macrophage cells, and suckling laboratory mice. Isolation sensitivity was similar in all methods tested. Laboratory mice and Huh-7 cells were less affected by toxicity from breast milk than Vero E6 and MDM cells. However, the advantages associated with isolation in Huh-7 cells over laboratory mice, including cost effectiveness, sample volume preservation, and a reduction in animal use, make Huh-7 cells the preferred substrate tested for Ebola virus Makona isolation. MDPI 2019-02-16 /pmc/articles/PMC6410085/ /pubmed/30781518 http://dx.doi.org/10.3390/v11020161 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Logue, James Vargas Licona, Walter Cooper, Timothy K. Reeder, Becky Byrum, Russel Qin, Jing Deiuliis Murphy, Nicole Cong, Yu Bonilla, Amanda Sword, Jennifer Weaver, Wade Kocher, Gregory Olinger, Gene G. Jahrling, Peter B. Hensley, Lisa E. Bennett, Richard S. Ebola Virus Isolation Using Huh-7 Cells has Methodological Advantages and Similar Sensitivity to Isolation Using Other Cell Types and Suckling BALB/c Laboratory Mice |
title | Ebola Virus Isolation Using Huh-7 Cells has Methodological Advantages and Similar Sensitivity to Isolation Using Other Cell Types and Suckling BALB/c Laboratory Mice |
title_full | Ebola Virus Isolation Using Huh-7 Cells has Methodological Advantages and Similar Sensitivity to Isolation Using Other Cell Types and Suckling BALB/c Laboratory Mice |
title_fullStr | Ebola Virus Isolation Using Huh-7 Cells has Methodological Advantages and Similar Sensitivity to Isolation Using Other Cell Types and Suckling BALB/c Laboratory Mice |
title_full_unstemmed | Ebola Virus Isolation Using Huh-7 Cells has Methodological Advantages and Similar Sensitivity to Isolation Using Other Cell Types and Suckling BALB/c Laboratory Mice |
title_short | Ebola Virus Isolation Using Huh-7 Cells has Methodological Advantages and Similar Sensitivity to Isolation Using Other Cell Types and Suckling BALB/c Laboratory Mice |
title_sort | ebola virus isolation using huh-7 cells has methodological advantages and similar sensitivity to isolation using other cell types and suckling balb/c laboratory mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410085/ https://www.ncbi.nlm.nih.gov/pubmed/30781518 http://dx.doi.org/10.3390/v11020161 |
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