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Ebola Virus Isolation Using Huh-7 Cells has Methodological Advantages and Similar Sensitivity to Isolation Using Other Cell Types and Suckling BALB/c Laboratory Mice

Following the largest Ebola virus disease outbreak from 2013 to 2016, viral RNA has been detected in survivors from semen and breast milk long after disease recovery. However, as there have been few cases of sexual transmission, it is unclear whether every RNA positive fluid sample contains infectio...

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Autores principales: Logue, James, Vargas Licona, Walter, Cooper, Timothy K., Reeder, Becky, Byrum, Russel, Qin, Jing, Deiuliis Murphy, Nicole, Cong, Yu, Bonilla, Amanda, Sword, Jennifer, Weaver, Wade, Kocher, Gregory, Olinger, Gene G., Jahrling, Peter B., Hensley, Lisa E., Bennett, Richard S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410085/
https://www.ncbi.nlm.nih.gov/pubmed/30781518
http://dx.doi.org/10.3390/v11020161
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author Logue, James
Vargas Licona, Walter
Cooper, Timothy K.
Reeder, Becky
Byrum, Russel
Qin, Jing
Deiuliis Murphy, Nicole
Cong, Yu
Bonilla, Amanda
Sword, Jennifer
Weaver, Wade
Kocher, Gregory
Olinger, Gene G.
Jahrling, Peter B.
Hensley, Lisa E.
Bennett, Richard S.
author_facet Logue, James
Vargas Licona, Walter
Cooper, Timothy K.
Reeder, Becky
Byrum, Russel
Qin, Jing
Deiuliis Murphy, Nicole
Cong, Yu
Bonilla, Amanda
Sword, Jennifer
Weaver, Wade
Kocher, Gregory
Olinger, Gene G.
Jahrling, Peter B.
Hensley, Lisa E.
Bennett, Richard S.
author_sort Logue, James
collection PubMed
description Following the largest Ebola virus disease outbreak from 2013 to 2016, viral RNA has been detected in survivors from semen and breast milk long after disease recovery. However, as there have been few cases of sexual transmission, it is unclear whether every RNA positive fluid sample contains infectious virus. Virus isolation, typically using cell culture or animal models, can serve as a tool to determine the infectivity of patient samples. However, the sensitivity of these methods has not been assessed for the Ebola virus isolate, Makona. Described here is an efficiency comparison of Ebola virus Makona isolation using Vero E6, Huh-7, monocyte-derived macrophage cells, and suckling laboratory mice. Isolation sensitivity was similar in all methods tested. Laboratory mice and Huh-7 cells were less affected by toxicity from breast milk than Vero E6 and MDM cells. However, the advantages associated with isolation in Huh-7 cells over laboratory mice, including cost effectiveness, sample volume preservation, and a reduction in animal use, make Huh-7 cells the preferred substrate tested for Ebola virus Makona isolation.
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spelling pubmed-64100852019-04-01 Ebola Virus Isolation Using Huh-7 Cells has Methodological Advantages and Similar Sensitivity to Isolation Using Other Cell Types and Suckling BALB/c Laboratory Mice Logue, James Vargas Licona, Walter Cooper, Timothy K. Reeder, Becky Byrum, Russel Qin, Jing Deiuliis Murphy, Nicole Cong, Yu Bonilla, Amanda Sword, Jennifer Weaver, Wade Kocher, Gregory Olinger, Gene G. Jahrling, Peter B. Hensley, Lisa E. Bennett, Richard S. Viruses Article Following the largest Ebola virus disease outbreak from 2013 to 2016, viral RNA has been detected in survivors from semen and breast milk long after disease recovery. However, as there have been few cases of sexual transmission, it is unclear whether every RNA positive fluid sample contains infectious virus. Virus isolation, typically using cell culture or animal models, can serve as a tool to determine the infectivity of patient samples. However, the sensitivity of these methods has not been assessed for the Ebola virus isolate, Makona. Described here is an efficiency comparison of Ebola virus Makona isolation using Vero E6, Huh-7, monocyte-derived macrophage cells, and suckling laboratory mice. Isolation sensitivity was similar in all methods tested. Laboratory mice and Huh-7 cells were less affected by toxicity from breast milk than Vero E6 and MDM cells. However, the advantages associated with isolation in Huh-7 cells over laboratory mice, including cost effectiveness, sample volume preservation, and a reduction in animal use, make Huh-7 cells the preferred substrate tested for Ebola virus Makona isolation. MDPI 2019-02-16 /pmc/articles/PMC6410085/ /pubmed/30781518 http://dx.doi.org/10.3390/v11020161 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Logue, James
Vargas Licona, Walter
Cooper, Timothy K.
Reeder, Becky
Byrum, Russel
Qin, Jing
Deiuliis Murphy, Nicole
Cong, Yu
Bonilla, Amanda
Sword, Jennifer
Weaver, Wade
Kocher, Gregory
Olinger, Gene G.
Jahrling, Peter B.
Hensley, Lisa E.
Bennett, Richard S.
Ebola Virus Isolation Using Huh-7 Cells has Methodological Advantages and Similar Sensitivity to Isolation Using Other Cell Types and Suckling BALB/c Laboratory Mice
title Ebola Virus Isolation Using Huh-7 Cells has Methodological Advantages and Similar Sensitivity to Isolation Using Other Cell Types and Suckling BALB/c Laboratory Mice
title_full Ebola Virus Isolation Using Huh-7 Cells has Methodological Advantages and Similar Sensitivity to Isolation Using Other Cell Types and Suckling BALB/c Laboratory Mice
title_fullStr Ebola Virus Isolation Using Huh-7 Cells has Methodological Advantages and Similar Sensitivity to Isolation Using Other Cell Types and Suckling BALB/c Laboratory Mice
title_full_unstemmed Ebola Virus Isolation Using Huh-7 Cells has Methodological Advantages and Similar Sensitivity to Isolation Using Other Cell Types and Suckling BALB/c Laboratory Mice
title_short Ebola Virus Isolation Using Huh-7 Cells has Methodological Advantages and Similar Sensitivity to Isolation Using Other Cell Types and Suckling BALB/c Laboratory Mice
title_sort ebola virus isolation using huh-7 cells has methodological advantages and similar sensitivity to isolation using other cell types and suckling balb/c laboratory mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410085/
https://www.ncbi.nlm.nih.gov/pubmed/30781518
http://dx.doi.org/10.3390/v11020161
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