Cargando…

The Influence of Envelope C-Terminus Amino Acid Composition on the Ratio of Cell-Free to Cell-Cell Transmission for Bovine Foamy Virus

Foamy viruses (FVs) have extensive cell tropism in vitro, special replication features, and no clinical pathogenicity in naturally or experimentally infected animals, which distinguish them from orthoretroviruses. Among FVs, bovine foamy virus (BFV) has undetectable or extremely low levels of cell-f...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Suzhen, Liu, Xiaojuan, Liang, Zhibin, Bing, Tiejun, Qiao, Wentao, Tan, Juan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410131/
https://www.ncbi.nlm.nih.gov/pubmed/30708993
http://dx.doi.org/10.3390/v11020130
_version_ 1783402167180197888
author Zhang, Suzhen
Liu, Xiaojuan
Liang, Zhibin
Bing, Tiejun
Qiao, Wentao
Tan, Juan
author_facet Zhang, Suzhen
Liu, Xiaojuan
Liang, Zhibin
Bing, Tiejun
Qiao, Wentao
Tan, Juan
author_sort Zhang, Suzhen
collection PubMed
description Foamy viruses (FVs) have extensive cell tropism in vitro, special replication features, and no clinical pathogenicity in naturally or experimentally infected animals, which distinguish them from orthoretroviruses. Among FVs, bovine foamy virus (BFV) has undetectable or extremely low levels of cell-free transmission in the supernatants of infected cells and mainly spreads by cell-to-cell transmission, which deters its use as a gene transfer vector. Here, using an in vitro virus evolution system, we successfully isolated high-titer cell-free BFV strains from the original cell-to-cell transmissible BFV3026 strain and further constructed an infectious cell-free BFV clone called pBS-BFV-Z1. Following sequence alignment with a cell-associated clone pBS-BFV-B, we identified a number of changes in the genome of pBS-BFV-Z1. Extensive mutagenesis analysis revealed that the C-terminus of envelope protein, especially the K898 residue, controls BFV cell-free transmission by enhancing cell-free virus entry but not the virus release capacity. Taken together, our data show the genetic determinants that regulate cell-to-cell and cell-free transmission of BFV.
format Online
Article
Text
id pubmed-6410131
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-64101312019-04-01 The Influence of Envelope C-Terminus Amino Acid Composition on the Ratio of Cell-Free to Cell-Cell Transmission for Bovine Foamy Virus Zhang, Suzhen Liu, Xiaojuan Liang, Zhibin Bing, Tiejun Qiao, Wentao Tan, Juan Viruses Article Foamy viruses (FVs) have extensive cell tropism in vitro, special replication features, and no clinical pathogenicity in naturally or experimentally infected animals, which distinguish them from orthoretroviruses. Among FVs, bovine foamy virus (BFV) has undetectable or extremely low levels of cell-free transmission in the supernatants of infected cells and mainly spreads by cell-to-cell transmission, which deters its use as a gene transfer vector. Here, using an in vitro virus evolution system, we successfully isolated high-titer cell-free BFV strains from the original cell-to-cell transmissible BFV3026 strain and further constructed an infectious cell-free BFV clone called pBS-BFV-Z1. Following sequence alignment with a cell-associated clone pBS-BFV-B, we identified a number of changes in the genome of pBS-BFV-Z1. Extensive mutagenesis analysis revealed that the C-terminus of envelope protein, especially the K898 residue, controls BFV cell-free transmission by enhancing cell-free virus entry but not the virus release capacity. Taken together, our data show the genetic determinants that regulate cell-to-cell and cell-free transmission of BFV. MDPI 2019-01-31 /pmc/articles/PMC6410131/ /pubmed/30708993 http://dx.doi.org/10.3390/v11020130 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Suzhen
Liu, Xiaojuan
Liang, Zhibin
Bing, Tiejun
Qiao, Wentao
Tan, Juan
The Influence of Envelope C-Terminus Amino Acid Composition on the Ratio of Cell-Free to Cell-Cell Transmission for Bovine Foamy Virus
title The Influence of Envelope C-Terminus Amino Acid Composition on the Ratio of Cell-Free to Cell-Cell Transmission for Bovine Foamy Virus
title_full The Influence of Envelope C-Terminus Amino Acid Composition on the Ratio of Cell-Free to Cell-Cell Transmission for Bovine Foamy Virus
title_fullStr The Influence of Envelope C-Terminus Amino Acid Composition on the Ratio of Cell-Free to Cell-Cell Transmission for Bovine Foamy Virus
title_full_unstemmed The Influence of Envelope C-Terminus Amino Acid Composition on the Ratio of Cell-Free to Cell-Cell Transmission for Bovine Foamy Virus
title_short The Influence of Envelope C-Terminus Amino Acid Composition on the Ratio of Cell-Free to Cell-Cell Transmission for Bovine Foamy Virus
title_sort influence of envelope c-terminus amino acid composition on the ratio of cell-free to cell-cell transmission for bovine foamy virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410131/
https://www.ncbi.nlm.nih.gov/pubmed/30708993
http://dx.doi.org/10.3390/v11020130
work_keys_str_mv AT zhangsuzhen theinfluenceofenvelopecterminusaminoacidcompositionontheratioofcellfreetocellcelltransmissionforbovinefoamyvirus
AT liuxiaojuan theinfluenceofenvelopecterminusaminoacidcompositionontheratioofcellfreetocellcelltransmissionforbovinefoamyvirus
AT liangzhibin theinfluenceofenvelopecterminusaminoacidcompositionontheratioofcellfreetocellcelltransmissionforbovinefoamyvirus
AT bingtiejun theinfluenceofenvelopecterminusaminoacidcompositionontheratioofcellfreetocellcelltransmissionforbovinefoamyvirus
AT qiaowentao theinfluenceofenvelopecterminusaminoacidcompositionontheratioofcellfreetocellcelltransmissionforbovinefoamyvirus
AT tanjuan theinfluenceofenvelopecterminusaminoacidcompositionontheratioofcellfreetocellcelltransmissionforbovinefoamyvirus
AT zhangsuzhen influenceofenvelopecterminusaminoacidcompositionontheratioofcellfreetocellcelltransmissionforbovinefoamyvirus
AT liuxiaojuan influenceofenvelopecterminusaminoacidcompositionontheratioofcellfreetocellcelltransmissionforbovinefoamyvirus
AT liangzhibin influenceofenvelopecterminusaminoacidcompositionontheratioofcellfreetocellcelltransmissionforbovinefoamyvirus
AT bingtiejun influenceofenvelopecterminusaminoacidcompositionontheratioofcellfreetocellcelltransmissionforbovinefoamyvirus
AT qiaowentao influenceofenvelopecterminusaminoacidcompositionontheratioofcellfreetocellcelltransmissionforbovinefoamyvirus
AT tanjuan influenceofenvelopecterminusaminoacidcompositionontheratioofcellfreetocellcelltransmissionforbovinefoamyvirus