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Chronic In Vivo Effects of Repeated Exposure to Low Oral Doses of Tetrodotoxin: Preliminary Evidence of Nephrotoxicity and Cardiotoxicity

Tetrodotoxin (TTX) is one of the most potent naturally occurring neurotoxins. Initially TTX was associated with human food intoxications in Japan, but nowadays, concerns about the human health risks posed by TTX have increased in Europe after the identification of the toxin in fish, marine gastropod...

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Autores principales: Boente-Juncal, Andrea, Vale, Carmen, Cifuentes, Manuel, Otero, Paz, Camiña, Mercedes, Rodriguez-Vieytes, Mercedes, Botana, Luis Miguel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410189/
https://www.ncbi.nlm.nih.gov/pubmed/30736354
http://dx.doi.org/10.3390/toxins11020096
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author Boente-Juncal, Andrea
Vale, Carmen
Cifuentes, Manuel
Otero, Paz
Camiña, Mercedes
Rodriguez-Vieytes, Mercedes
Botana, Luis Miguel
author_facet Boente-Juncal, Andrea
Vale, Carmen
Cifuentes, Manuel
Otero, Paz
Camiña, Mercedes
Rodriguez-Vieytes, Mercedes
Botana, Luis Miguel
author_sort Boente-Juncal, Andrea
collection PubMed
description Tetrodotoxin (TTX) is one of the most potent naturally occurring neurotoxins. Initially TTX was associated with human food intoxications in Japan, but nowadays, concerns about the human health risks posed by TTX have increased in Europe after the identification of the toxin in fish, marine gastropods, and bivalves captured in European waters. Even when TTX monitoring is not currently performed in Europe, an acute oral no observable effect level (NOAEL) of 75 μg/kg has been recently established but, to date, no studies evaluating the chronic oral toxicity of TTX have been released, even when EFSA has highlighted the need for them. Thus, in this work, the chronic effects of low oral TTX doses (below the acute lethal dose 50) were evaluated following internationally adopted guidelines. The results presented here demonstrate that low oral doses of TTX have deleterious effects on renal and cardiac tissues. Moreover, alterations in blood biochemistry parameters, urine production, and urinalysis data were already detected at the oral dose of 75 µg/kg after the 28 days exposure. Thus, the data presented here constitute an initial approach for the chronic evaluation of the in vivo toxicity of tetrodotoxin after its ingestion through contaminated fishery products.
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spelling pubmed-64101892019-04-01 Chronic In Vivo Effects of Repeated Exposure to Low Oral Doses of Tetrodotoxin: Preliminary Evidence of Nephrotoxicity and Cardiotoxicity Boente-Juncal, Andrea Vale, Carmen Cifuentes, Manuel Otero, Paz Camiña, Mercedes Rodriguez-Vieytes, Mercedes Botana, Luis Miguel Toxins (Basel) Article Tetrodotoxin (TTX) is one of the most potent naturally occurring neurotoxins. Initially TTX was associated with human food intoxications in Japan, but nowadays, concerns about the human health risks posed by TTX have increased in Europe after the identification of the toxin in fish, marine gastropods, and bivalves captured in European waters. Even when TTX monitoring is not currently performed in Europe, an acute oral no observable effect level (NOAEL) of 75 μg/kg has been recently established but, to date, no studies evaluating the chronic oral toxicity of TTX have been released, even when EFSA has highlighted the need for them. Thus, in this work, the chronic effects of low oral TTX doses (below the acute lethal dose 50) were evaluated following internationally adopted guidelines. The results presented here demonstrate that low oral doses of TTX have deleterious effects on renal and cardiac tissues. Moreover, alterations in blood biochemistry parameters, urine production, and urinalysis data were already detected at the oral dose of 75 µg/kg after the 28 days exposure. Thus, the data presented here constitute an initial approach for the chronic evaluation of the in vivo toxicity of tetrodotoxin after its ingestion through contaminated fishery products. MDPI 2019-02-06 /pmc/articles/PMC6410189/ /pubmed/30736354 http://dx.doi.org/10.3390/toxins11020096 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Boente-Juncal, Andrea
Vale, Carmen
Cifuentes, Manuel
Otero, Paz
Camiña, Mercedes
Rodriguez-Vieytes, Mercedes
Botana, Luis Miguel
Chronic In Vivo Effects of Repeated Exposure to Low Oral Doses of Tetrodotoxin: Preliminary Evidence of Nephrotoxicity and Cardiotoxicity
title Chronic In Vivo Effects of Repeated Exposure to Low Oral Doses of Tetrodotoxin: Preliminary Evidence of Nephrotoxicity and Cardiotoxicity
title_full Chronic In Vivo Effects of Repeated Exposure to Low Oral Doses of Tetrodotoxin: Preliminary Evidence of Nephrotoxicity and Cardiotoxicity
title_fullStr Chronic In Vivo Effects of Repeated Exposure to Low Oral Doses of Tetrodotoxin: Preliminary Evidence of Nephrotoxicity and Cardiotoxicity
title_full_unstemmed Chronic In Vivo Effects of Repeated Exposure to Low Oral Doses of Tetrodotoxin: Preliminary Evidence of Nephrotoxicity and Cardiotoxicity
title_short Chronic In Vivo Effects of Repeated Exposure to Low Oral Doses of Tetrodotoxin: Preliminary Evidence of Nephrotoxicity and Cardiotoxicity
title_sort chronic in vivo effects of repeated exposure to low oral doses of tetrodotoxin: preliminary evidence of nephrotoxicity and cardiotoxicity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410189/
https://www.ncbi.nlm.nih.gov/pubmed/30736354
http://dx.doi.org/10.3390/toxins11020096
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