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Synthetic metabolic pathway for the production of 1-alkenes from lignin-derived molecules

BACKGROUND: Integration of synthetic metabolic pathways to catabolically diverse chassis provides new opportunities for sustainable production. One attractive scenario is the use of abundant waste material to produce a readily collectable product, which can reduce the production costs. Towards that...

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Autores principales: Luo, Jin, Lehtinen, Tapio, Efimova, Elena, Santala, Ville, Santala, Suvi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410514/
https://www.ncbi.nlm.nih.gov/pubmed/30857542
http://dx.doi.org/10.1186/s12934-019-1097-x
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author Luo, Jin
Lehtinen, Tapio
Efimova, Elena
Santala, Ville
Santala, Suvi
author_facet Luo, Jin
Lehtinen, Tapio
Efimova, Elena
Santala, Ville
Santala, Suvi
author_sort Luo, Jin
collection PubMed
description BACKGROUND: Integration of synthetic metabolic pathways to catabolically diverse chassis provides new opportunities for sustainable production. One attractive scenario is the use of abundant waste material to produce a readily collectable product, which can reduce the production costs. Towards that end, we established a cellular platform for the production of semivolatile medium-chain α-olefins from lignin-derived molecules: we constructed 1-undecene synthesis pathway in Acinetobacter baylyi ADP1 using ferulate, a lignin-derived model compound, as the sole carbon source for both cell growth and product synthesis. RESULTS: In order to overcome the toxicity of ferulate, we first applied adaptive laboratory evolution to A. baylyi ADP1, resulting in a highly ferulate-tolerant strain. The adapted strain exhibited robust growth in 100 mM ferulate while the growth of the wild type strain was completely inhibited. Next, we expressed two heterologous enzymes in the wild type strain to confer 1-undecene production from glucose: a fatty acid decarboxylase UndA from Pseudomonas putida, and a thioesterase ‘TesA from Escherichia coli. Finally, we constructed the 1-undecene synthesis pathway in the ferulate-tolerant strain. The engineered cells were able to produce biomass and 1-undecene solely from ferulate, and excreted the product directly to the culture headspace. CONCLUSIONS: In this study, we employed a bacterium Acinetobacter baylyi ADP1 to integrate a natural aromatics degrading pathway to a synthetic production route, allowing the upgradation of lignin derived molecules to value-added products. We developed a highly ferulate-tolerant strain and established the biosynthesis of an industrially relevant chemical, 1-undecene, solely from the lignin-derived model compound. This study reports the production of alkenes from lignin derived molecules for the first time and demonstrates the potential of lignin as a sustainable resource in the bio-based synthesis of valuable products. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12934-019-1097-x) contains supplementary material, which is available to authorized users.
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spelling pubmed-64105142019-03-21 Synthetic metabolic pathway for the production of 1-alkenes from lignin-derived molecules Luo, Jin Lehtinen, Tapio Efimova, Elena Santala, Ville Santala, Suvi Microb Cell Fact Research BACKGROUND: Integration of synthetic metabolic pathways to catabolically diverse chassis provides new opportunities for sustainable production. One attractive scenario is the use of abundant waste material to produce a readily collectable product, which can reduce the production costs. Towards that end, we established a cellular platform for the production of semivolatile medium-chain α-olefins from lignin-derived molecules: we constructed 1-undecene synthesis pathway in Acinetobacter baylyi ADP1 using ferulate, a lignin-derived model compound, as the sole carbon source for both cell growth and product synthesis. RESULTS: In order to overcome the toxicity of ferulate, we first applied adaptive laboratory evolution to A. baylyi ADP1, resulting in a highly ferulate-tolerant strain. The adapted strain exhibited robust growth in 100 mM ferulate while the growth of the wild type strain was completely inhibited. Next, we expressed two heterologous enzymes in the wild type strain to confer 1-undecene production from glucose: a fatty acid decarboxylase UndA from Pseudomonas putida, and a thioesterase ‘TesA from Escherichia coli. Finally, we constructed the 1-undecene synthesis pathway in the ferulate-tolerant strain. The engineered cells were able to produce biomass and 1-undecene solely from ferulate, and excreted the product directly to the culture headspace. CONCLUSIONS: In this study, we employed a bacterium Acinetobacter baylyi ADP1 to integrate a natural aromatics degrading pathway to a synthetic production route, allowing the upgradation of lignin derived molecules to value-added products. We developed a highly ferulate-tolerant strain and established the biosynthesis of an industrially relevant chemical, 1-undecene, solely from the lignin-derived model compound. This study reports the production of alkenes from lignin derived molecules for the first time and demonstrates the potential of lignin as a sustainable resource in the bio-based synthesis of valuable products. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12934-019-1097-x) contains supplementary material, which is available to authorized users. BioMed Central 2019-03-11 /pmc/articles/PMC6410514/ /pubmed/30857542 http://dx.doi.org/10.1186/s12934-019-1097-x Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Luo, Jin
Lehtinen, Tapio
Efimova, Elena
Santala, Ville
Santala, Suvi
Synthetic metabolic pathway for the production of 1-alkenes from lignin-derived molecules
title Synthetic metabolic pathway for the production of 1-alkenes from lignin-derived molecules
title_full Synthetic metabolic pathway for the production of 1-alkenes from lignin-derived molecules
title_fullStr Synthetic metabolic pathway for the production of 1-alkenes from lignin-derived molecules
title_full_unstemmed Synthetic metabolic pathway for the production of 1-alkenes from lignin-derived molecules
title_short Synthetic metabolic pathway for the production of 1-alkenes from lignin-derived molecules
title_sort synthetic metabolic pathway for the production of 1-alkenes from lignin-derived molecules
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410514/
https://www.ncbi.nlm.nih.gov/pubmed/30857542
http://dx.doi.org/10.1186/s12934-019-1097-x
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