Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system

BACKGROUND: The hemolysin (Hly) secretion system of E. coli allows the one-step translocation of hemolysin A (HlyA) from the bacterial cytoplasm to the extracellular medium, without a periplasmic intermediate. In this work, we investigate whether the Hly secretion system of E. coli is competent to s...

Descripción completa

Detalles Bibliográficos
Autores principales: Ruano-Gallego, David, Fraile, Sofía, Gutierrez, Carlos, Fernández, Luis Ángel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410518/
https://www.ncbi.nlm.nih.gov/pubmed/30857538
http://dx.doi.org/10.1186/s12934-019-1094-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410518/
https://www.ncbi.nlm.nih.gov/pubmed/30857538
http://dx.doi.org/10.1186/s12934-019-1094-0

Ejemplares similares