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Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system
BACKGROUND: The hemolysin (Hly) secretion system of E. coli allows the one-step translocation of hemolysin A (HlyA) from the bacterial cytoplasm to the extracellular medium, without a periplasmic intermediate. In this work, we investigate whether the Hly secretion system of E. coli is competent to s...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410518/ https://www.ncbi.nlm.nih.gov/pubmed/30857538 http://dx.doi.org/10.1186/s12934-019-1094-0 |