Cargando…
MTHFR C677T polymorphism and risk of nonsyndromic cleft lip with or without cleft palate in the Moroccan population
BACKGROUND: Nonsyndromic cleft lip with or without cleft palate (NSCL/P) is one of the most common craniofacial malformations observed. Several studies suggest that the decrease in folate has been associated with a higher risk of NSCL/P. The present study aimed to determine the association of 5,10-m...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410759/ https://www.ncbi.nlm.nih.gov/pubmed/30881086 http://dx.doi.org/10.2147/TACG.S194166 |
_version_ | 1783402307544678400 |
---|---|
author | Rafik, Amine Rachad, Laila Kone, Abdou-samad Nadifi, Sellama |
author_facet | Rafik, Amine Rachad, Laila Kone, Abdou-samad Nadifi, Sellama |
author_sort | Rafik, Amine |
collection | PubMed |
description | BACKGROUND: Nonsyndromic cleft lip with or without cleft palate (NSCL/P) is one of the most common craniofacial malformations observed. Several studies suggest that the decrease in folate has been associated with a higher risk of NSCL/P. The present study aimed to determine the association of 5,10-methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism gene with the occurrence of NSCL/P in the Moroccan population. METHODS: MTHFR C677T was genotyped in 52 Moroccan patients and 182 unrelated controls, using a PCR followed by restriction fragment length polymorphism. RESULTS: The results of the study revealed a genotypic and phenotypic distribution in equilibrium with Hardy–Weinberg’s law (χ(2)=0.36, P=0.55). The frequency of heterozygous genotype C/T and the T allele in controls and patients were 40.7% vs 15.4% and 26%, respectively. CONCLUSION: A low association was found between the C677T polymorphism of the MTHFR gene and a risk for the development of NSCL/P in the Moroccan population (OR =0.24, P=0.0005). |
format | Online Article Text |
id | pubmed-6410759 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-64107592019-03-16 MTHFR C677T polymorphism and risk of nonsyndromic cleft lip with or without cleft palate in the Moroccan population Rafik, Amine Rachad, Laila Kone, Abdou-samad Nadifi, Sellama Appl Clin Genet Original Research BACKGROUND: Nonsyndromic cleft lip with or without cleft palate (NSCL/P) is one of the most common craniofacial malformations observed. Several studies suggest that the decrease in folate has been associated with a higher risk of NSCL/P. The present study aimed to determine the association of 5,10-methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism gene with the occurrence of NSCL/P in the Moroccan population. METHODS: MTHFR C677T was genotyped in 52 Moroccan patients and 182 unrelated controls, using a PCR followed by restriction fragment length polymorphism. RESULTS: The results of the study revealed a genotypic and phenotypic distribution in equilibrium with Hardy–Weinberg’s law (χ(2)=0.36, P=0.55). The frequency of heterozygous genotype C/T and the T allele in controls and patients were 40.7% vs 15.4% and 26%, respectively. CONCLUSION: A low association was found between the C677T polymorphism of the MTHFR gene and a risk for the development of NSCL/P in the Moroccan population (OR =0.24, P=0.0005). Dove Medical Press 2019-03-07 /pmc/articles/PMC6410759/ /pubmed/30881086 http://dx.doi.org/10.2147/TACG.S194166 Text en © 2019 Rafik et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Rafik, Amine Rachad, Laila Kone, Abdou-samad Nadifi, Sellama MTHFR C677T polymorphism and risk of nonsyndromic cleft lip with or without cleft palate in the Moroccan population |
title | MTHFR C677T polymorphism and risk of nonsyndromic cleft lip with or without cleft palate in the Moroccan population |
title_full | MTHFR C677T polymorphism and risk of nonsyndromic cleft lip with or without cleft palate in the Moroccan population |
title_fullStr | MTHFR C677T polymorphism and risk of nonsyndromic cleft lip with or without cleft palate in the Moroccan population |
title_full_unstemmed | MTHFR C677T polymorphism and risk of nonsyndromic cleft lip with or without cleft palate in the Moroccan population |
title_short | MTHFR C677T polymorphism and risk of nonsyndromic cleft lip with or without cleft palate in the Moroccan population |
title_sort | mthfr c677t polymorphism and risk of nonsyndromic cleft lip with or without cleft palate in the moroccan population |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410759/ https://www.ncbi.nlm.nih.gov/pubmed/30881086 http://dx.doi.org/10.2147/TACG.S194166 |
work_keys_str_mv | AT rafikamine mthfrc677tpolymorphismandriskofnonsyndromiccleftlipwithorwithoutcleftpalateinthemoroccanpopulation AT rachadlaila mthfrc677tpolymorphismandriskofnonsyndromiccleftlipwithorwithoutcleftpalateinthemoroccanpopulation AT koneabdousamad mthfrc677tpolymorphismandriskofnonsyndromiccleftlipwithorwithoutcleftpalateinthemoroccanpopulation AT nadifisellama mthfrc677tpolymorphismandriskofnonsyndromiccleftlipwithorwithoutcleftpalateinthemoroccanpopulation |