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Oleic acid induces migration through a FFAR1/4, EGFR and AKT-dependent pathway in breast cancer cells
Free fatty acids (FFAs) are an energy source, and induce activation of signal transduction pathways that mediate several biological processes. In breast cancer cells, oleic acid (OA) induces proliferation, matrix metalloproteinase-9 (MMP-9) secretion, migration and invasion. However, the signal tran...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Bioscientifica Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410766/ https://www.ncbi.nlm.nih.gov/pubmed/30721135 http://dx.doi.org/10.1530/EC-18-0543 |
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author | Marcial-Medina, Cleofas Ordoñez-Moreno, Alejandra Gonzalez-Reyes, Christian Cortes-Reynosa, Pedro Perez Salazar, Eduardo |
author_facet | Marcial-Medina, Cleofas Ordoñez-Moreno, Alejandra Gonzalez-Reyes, Christian Cortes-Reynosa, Pedro Perez Salazar, Eduardo |
author_sort | Marcial-Medina, Cleofas |
collection | PubMed |
description | Free fatty acids (FFAs) are an energy source, and induce activation of signal transduction pathways that mediate several biological processes. In breast cancer cells, oleic acid (OA) induces proliferation, matrix metalloproteinase-9 (MMP-9) secretion, migration and invasion. However, the signal transduction pathways that mediate migration and invasion induced by OA in breast cancer cells have not been studied in detail. We demonstrate here that FFAR1 and FFAR4 mediate migration induced by OA in MDA-MB-231 and MCF-7 breast cancer cells. Moreover, OA induces migration, invasion, AKT1 and AKT2 activation, 12-LOX secretion and an increase of NFκB-DNA binding activity in breast cancer cells. Cell migration requires FFAR1, FFAR4, EGFR, AKT and PI3K activity, whereas invasion is mediated though a PI3K/Akt-dependent pathway. Furthermore, OA promotes relocalization of paxillin to focal contacts and it requires PI3K and EGFR activity, whereas NFκB-DNA binding activity requires PI3K and AKT activity. |
format | Online Article Text |
id | pubmed-6410766 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Bioscientifica Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-64107662019-03-14 Oleic acid induces migration through a FFAR1/4, EGFR and AKT-dependent pathway in breast cancer cells Marcial-Medina, Cleofas Ordoñez-Moreno, Alejandra Gonzalez-Reyes, Christian Cortes-Reynosa, Pedro Perez Salazar, Eduardo Endocr Connect Research Free fatty acids (FFAs) are an energy source, and induce activation of signal transduction pathways that mediate several biological processes. In breast cancer cells, oleic acid (OA) induces proliferation, matrix metalloproteinase-9 (MMP-9) secretion, migration and invasion. However, the signal transduction pathways that mediate migration and invasion induced by OA in breast cancer cells have not been studied in detail. We demonstrate here that FFAR1 and FFAR4 mediate migration induced by OA in MDA-MB-231 and MCF-7 breast cancer cells. Moreover, OA induces migration, invasion, AKT1 and AKT2 activation, 12-LOX secretion and an increase of NFκB-DNA binding activity in breast cancer cells. Cell migration requires FFAR1, FFAR4, EGFR, AKT and PI3K activity, whereas invasion is mediated though a PI3K/Akt-dependent pathway. Furthermore, OA promotes relocalization of paxillin to focal contacts and it requires PI3K and EGFR activity, whereas NFκB-DNA binding activity requires PI3K and AKT activity. Bioscientifica Ltd 2019-02-04 /pmc/articles/PMC6410766/ /pubmed/30721135 http://dx.doi.org/10.1530/EC-18-0543 Text en © 2019 The authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Research Marcial-Medina, Cleofas Ordoñez-Moreno, Alejandra Gonzalez-Reyes, Christian Cortes-Reynosa, Pedro Perez Salazar, Eduardo Oleic acid induces migration through a FFAR1/4, EGFR and AKT-dependent pathway in breast cancer cells |
title | Oleic acid induces migration through a FFAR1/4, EGFR and AKT-dependent pathway in breast cancer cells |
title_full | Oleic acid induces migration through a FFAR1/4, EGFR and AKT-dependent pathway in breast cancer cells |
title_fullStr | Oleic acid induces migration through a FFAR1/4, EGFR and AKT-dependent pathway in breast cancer cells |
title_full_unstemmed | Oleic acid induces migration through a FFAR1/4, EGFR and AKT-dependent pathway in breast cancer cells |
title_short | Oleic acid induces migration through a FFAR1/4, EGFR and AKT-dependent pathway in breast cancer cells |
title_sort | oleic acid induces migration through a ffar1/4, egfr and akt-dependent pathway in breast cancer cells |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410766/ https://www.ncbi.nlm.nih.gov/pubmed/30721135 http://dx.doi.org/10.1530/EC-18-0543 |
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