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RNAi-dependent Polycomb repression controls transposable elements in Tetrahymena
RNAi and Polycomb repression play evolutionarily conserved and often coordinated roles in transcriptional silencing. Here, we show that, in the protozoan Tetrahymena thermophila, germline-specific internally eliminated sequences (IESs)—many related to transposable elements (TEs)—become transcription...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411011/ https://www.ncbi.nlm.nih.gov/pubmed/30808657 http://dx.doi.org/10.1101/gad.320796.118 |
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author | Zhao, Xiaolu Xiong, Jie Mao, Fengbiao Sheng, Yalan Chen, Xiao Feng, Lifang Dui, Wen Yang, Wentao Kapusta, Aurélie Feschotte, Cédric Coyne, Robert S. Miao, Wei Gao, Shan Liu, Yifan |
author_facet | Zhao, Xiaolu Xiong, Jie Mao, Fengbiao Sheng, Yalan Chen, Xiao Feng, Lifang Dui, Wen Yang, Wentao Kapusta, Aurélie Feschotte, Cédric Coyne, Robert S. Miao, Wei Gao, Shan Liu, Yifan |
author_sort | Zhao, Xiaolu |
collection | PubMed |
description | RNAi and Polycomb repression play evolutionarily conserved and often coordinated roles in transcriptional silencing. Here, we show that, in the protozoan Tetrahymena thermophila, germline-specific internally eliminated sequences (IESs)—many related to transposable elements (TEs)—become transcriptionally activated in mutants deficient in the RNAi-dependent Polycomb repression pathway. Germline TE mobilization also dramatically increases in these mutants. The transition from noncoding RNA (ncRNA) to mRNA production accompanies transcriptional activation of TE-related sequences and vice versa for transcriptional silencing. The balance between ncRNA and mRNA production is potentially affected by cotranscriptional processing as well as RNAi and Polycomb repression. We posit that interplay between RNAi and Polycomb repression is a widely conserved phenomenon, whose ancestral role is epigenetic silencing of TEs. |
format | Online Article Text |
id | pubmed-6411011 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-64110112019-09-01 RNAi-dependent Polycomb repression controls transposable elements in Tetrahymena Zhao, Xiaolu Xiong, Jie Mao, Fengbiao Sheng, Yalan Chen, Xiao Feng, Lifang Dui, Wen Yang, Wentao Kapusta, Aurélie Feschotte, Cédric Coyne, Robert S. Miao, Wei Gao, Shan Liu, Yifan Genes Dev Research Paper RNAi and Polycomb repression play evolutionarily conserved and often coordinated roles in transcriptional silencing. Here, we show that, in the protozoan Tetrahymena thermophila, germline-specific internally eliminated sequences (IESs)—many related to transposable elements (TEs)—become transcriptionally activated in mutants deficient in the RNAi-dependent Polycomb repression pathway. Germline TE mobilization also dramatically increases in these mutants. The transition from noncoding RNA (ncRNA) to mRNA production accompanies transcriptional activation of TE-related sequences and vice versa for transcriptional silencing. The balance between ncRNA and mRNA production is potentially affected by cotranscriptional processing as well as RNAi and Polycomb repression. We posit that interplay between RNAi and Polycomb repression is a widely conserved phenomenon, whose ancestral role is epigenetic silencing of TEs. Cold Spring Harbor Laboratory Press 2019-03-01 /pmc/articles/PMC6411011/ /pubmed/30808657 http://dx.doi.org/10.1101/gad.320796.118 Text en © 2019 Zhao et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. |
spellingShingle | Research Paper Zhao, Xiaolu Xiong, Jie Mao, Fengbiao Sheng, Yalan Chen, Xiao Feng, Lifang Dui, Wen Yang, Wentao Kapusta, Aurélie Feschotte, Cédric Coyne, Robert S. Miao, Wei Gao, Shan Liu, Yifan RNAi-dependent Polycomb repression controls transposable elements in Tetrahymena |
title | RNAi-dependent Polycomb repression controls transposable elements in Tetrahymena |
title_full | RNAi-dependent Polycomb repression controls transposable elements in Tetrahymena |
title_fullStr | RNAi-dependent Polycomb repression controls transposable elements in Tetrahymena |
title_full_unstemmed | RNAi-dependent Polycomb repression controls transposable elements in Tetrahymena |
title_short | RNAi-dependent Polycomb repression controls transposable elements in Tetrahymena |
title_sort | rnai-dependent polycomb repression controls transposable elements in tetrahymena |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411011/ https://www.ncbi.nlm.nih.gov/pubmed/30808657 http://dx.doi.org/10.1101/gad.320796.118 |
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