Resolution of human ribosomal DNA occurs in anaphase, dependent on tankyrase 1, condensin II, and topoisomerase IIα

Formation of individualized sister chromatids is essential for their accurate segregation. In budding yeast, while most of the genome segregates at the metaphase to anaphase transition, resolution of the ribosomal DNA (rDNA) repeats is delayed. The timing and mechanism in human cells is unknown. Her...

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Detalles Bibliográficos
Autores principales: Daniloski, Zharko, Bisht, Kamlesh K., McStay, Brian, Smith, Susan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2019
Materias:
Research Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411013/
https://www.ncbi.nlm.nih.gov/pubmed/30804226
http://dx.doi.org/10.1101/gad.321836.118
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author Daniloski, Zharko
Bisht, Kamlesh K.
McStay, Brian
Smith, Susan
author_facet Daniloski, Zharko
Bisht, Kamlesh K.
McStay, Brian
Smith, Susan
author_sort Daniloski, Zharko
collection PubMed
description Formation of individualized sister chromatids is essential for their accurate segregation. In budding yeast, while most of the genome segregates at the metaphase to anaphase transition, resolution of the ribosomal DNA (rDNA) repeats is delayed. The timing and mechanism in human cells is unknown. Here we show that resolution of human rDNA occurs in anaphase after the bulk of the genome, dependent on tankyrase 1, condensin II, and topoisomerase IIα. Defective resolution leads to rDNA bridges, rDNA damage, and aneuploidy of an rDNA-containing acrocentric chromosome. Thus, temporal regulation of rDNA segregation is conserved between yeast and man and is essential for genome integrity.
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spelling pubmed-64110132019-09-01 Resolution of human ribosomal DNA occurs in anaphase, dependent on tankyrase 1, condensin II, and topoisomerase IIα Daniloski, Zharko Bisht, Kamlesh K. McStay, Brian Smith, Susan Genes Dev Research Communication Formation of individualized sister chromatids is essential for their accurate segregation. In budding yeast, while most of the genome segregates at the metaphase to anaphase transition, resolution of the ribosomal DNA (rDNA) repeats is delayed. The timing and mechanism in human cells is unknown. Here we show that resolution of human rDNA occurs in anaphase after the bulk of the genome, dependent on tankyrase 1, condensin II, and topoisomerase IIα. Defective resolution leads to rDNA bridges, rDNA damage, and aneuploidy of an rDNA-containing acrocentric chromosome. Thus, temporal regulation of rDNA segregation is conserved between yeast and man and is essential for genome integrity. Cold Spring Harbor Laboratory Press 2019-03-01 /pmc/articles/PMC6411013/ /pubmed/30804226 http://dx.doi.org/10.1101/gad.321836.118 Text en © 2019 Daniloski et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research Communication
Daniloski, Zharko
Bisht, Kamlesh K.
McStay, Brian
Smith, Susan
Resolution of human ribosomal DNA occurs in anaphase, dependent on tankyrase 1, condensin II, and topoisomerase IIα
title Resolution of human ribosomal DNA occurs in anaphase, dependent on tankyrase 1, condensin II, and topoisomerase IIα
title_full Resolution of human ribosomal DNA occurs in anaphase, dependent on tankyrase 1, condensin II, and topoisomerase IIα
title_fullStr Resolution of human ribosomal DNA occurs in anaphase, dependent on tankyrase 1, condensin II, and topoisomerase IIα
title_full_unstemmed Resolution of human ribosomal DNA occurs in anaphase, dependent on tankyrase 1, condensin II, and topoisomerase IIα
title_short Resolution of human ribosomal DNA occurs in anaphase, dependent on tankyrase 1, condensin II, and topoisomerase IIα
title_sort resolution of human ribosomal dna occurs in anaphase, dependent on tankyrase 1, condensin ii, and topoisomerase iiα
topic Research Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411013/
https://www.ncbi.nlm.nih.gov/pubmed/30804226
http://dx.doi.org/10.1101/gad.321836.118
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