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The importance of extracellular vesicle purification for downstream analysis: A comparison of differential centrifugation and size exclusion chromatography for helminth pathogens

BACKGROUND: Robust protocols for the isolation of extracellular vesicles (EVs) from the rest of their excretory-secretory products are necessary for downstream studies and application development. The most widely used purification method of EVs for helminth pathogens is currently differential centri...

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Detalles Bibliográficos
Autores principales: Davis, Chelsea N., Phillips, Helen, Tomes, John J., Swain, Martin T., Wilkinson, Toby J., Brophy, Peter M., Morphew, Russell M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411213/
https://www.ncbi.nlm.nih.gov/pubmed/30811394
http://dx.doi.org/10.1371/journal.pntd.0007191