Cargando…
Homeostasis in the soybean miRNA396–GRF network is essential for productive soybean cyst nematode infections
Heterodera glycines, the soybean cyst nematode, penetrates soybean roots and migrates to the vascular cylinder where it forms a feeding site called the syncytium. MiRNA396 (miR396) targets growth-regulating factor (GRF) genes, and the miR396–GRF1/3 module is a master regulator of syncytium developme...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411377/ https://www.ncbi.nlm.nih.gov/pubmed/30715445 http://dx.doi.org/10.1093/jxb/erz022 |
_version_ | 1783402382896398336 |
---|---|
author | Noon, Jason B Hewezi, Tarek Baum, Thomas J |
author_facet | Noon, Jason B Hewezi, Tarek Baum, Thomas J |
author_sort | Noon, Jason B |
collection | PubMed |
description | Heterodera glycines, the soybean cyst nematode, penetrates soybean roots and migrates to the vascular cylinder where it forms a feeding site called the syncytium. MiRNA396 (miR396) targets growth-regulating factor (GRF) genes, and the miR396–GRF1/3 module is a master regulator of syncytium development in model cyst nematode H. schachtii infection of Arabidopsis. Here, we investigated whether this regulatory system operates similarly in soybean roots and is likewise important for H. glycines infection. We found that a network involving nine MIR396 and 23 GRF genes is important for normal development of soybean roots and that GRF function is specified in the root apical meristem by miR396. All MIR396 genes are down-regulated in the syncytium during its formation phase while, specifically, 11 different GRF genes are up-regulated. The switch to the syncytium maintenance phase coincides with up-regulation of MIR396 and down-regulation of the 11 GRF genes specifically via post-transcriptional regulation by miR396. Furthermore, interference with the miR396–GRF6/8–13/15–17/19 regulatory network, through either overexpression or knockdown experiments, does not affect the number of H. glycines juveniles that enter the vascular cylinder to initiate syncytia, but specifically inhibits efficient H. glycines development to adult females. Therefore, homeostasis in the miR396–GRF6/8–13/15–17/19 regulatory network is essential for productive H. glycines infections. |
format | Online Article Text |
id | pubmed-6411377 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-64113772019-03-15 Homeostasis in the soybean miRNA396–GRF network is essential for productive soybean cyst nematode infections Noon, Jason B Hewezi, Tarek Baum, Thomas J J Exp Bot Research Papers Heterodera glycines, the soybean cyst nematode, penetrates soybean roots and migrates to the vascular cylinder where it forms a feeding site called the syncytium. MiRNA396 (miR396) targets growth-regulating factor (GRF) genes, and the miR396–GRF1/3 module is a master regulator of syncytium development in model cyst nematode H. schachtii infection of Arabidopsis. Here, we investigated whether this regulatory system operates similarly in soybean roots and is likewise important for H. glycines infection. We found that a network involving nine MIR396 and 23 GRF genes is important for normal development of soybean roots and that GRF function is specified in the root apical meristem by miR396. All MIR396 genes are down-regulated in the syncytium during its formation phase while, specifically, 11 different GRF genes are up-regulated. The switch to the syncytium maintenance phase coincides with up-regulation of MIR396 and down-regulation of the 11 GRF genes specifically via post-transcriptional regulation by miR396. Furthermore, interference with the miR396–GRF6/8–13/15–17/19 regulatory network, through either overexpression or knockdown experiments, does not affect the number of H. glycines juveniles that enter the vascular cylinder to initiate syncytia, but specifically inhibits efficient H. glycines development to adult females. Therefore, homeostasis in the miR396–GRF6/8–13/15–17/19 regulatory network is essential for productive H. glycines infections. Oxford University Press 2019-02-15 2019-01-30 /pmc/articles/PMC6411377/ /pubmed/30715445 http://dx.doi.org/10.1093/jxb/erz022 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Papers Noon, Jason B Hewezi, Tarek Baum, Thomas J Homeostasis in the soybean miRNA396–GRF network is essential for productive soybean cyst nematode infections |
title | Homeostasis in the soybean miRNA396–GRF network is essential for productive soybean cyst nematode infections |
title_full | Homeostasis in the soybean miRNA396–GRF network is essential for productive soybean cyst nematode infections |
title_fullStr | Homeostasis in the soybean miRNA396–GRF network is essential for productive soybean cyst nematode infections |
title_full_unstemmed | Homeostasis in the soybean miRNA396–GRF network is essential for productive soybean cyst nematode infections |
title_short | Homeostasis in the soybean miRNA396–GRF network is essential for productive soybean cyst nematode infections |
title_sort | homeostasis in the soybean mirna396–grf network is essential for productive soybean cyst nematode infections |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411377/ https://www.ncbi.nlm.nih.gov/pubmed/30715445 http://dx.doi.org/10.1093/jxb/erz022 |
work_keys_str_mv | AT noonjasonb homeostasisinthesoybeanmirna396grfnetworkisessentialforproductivesoybeancystnematodeinfections AT hewezitarek homeostasisinthesoybeanmirna396grfnetworkisessentialforproductivesoybeancystnematodeinfections AT baumthomasj homeostasisinthesoybeanmirna396grfnetworkisessentialforproductivesoybeancystnematodeinfections |