Cargando…

Staging and prognosis of oropharyngeal carcinoma according to the 8th Edition of the American Joint Committee on Cancer Staging Manual in human papillomavirus infection

PURPOSE: The aim of this study was to evaluate the 8th edition of the American Joint Committee on Cancer Staging Manual: Head and Neck Section on oropharyngeal squamous cell cancer (OPSCC) and to clarify the relationship between p16 overexpression and the presence of human papillomavirus (HPV) DNA u...

Descripción completa

Detalles Bibliográficos
Autores principales: Yamashita, Yukashi, Ikegami, Taro, Hirakawa, Hitoshi, Uehara, Takayuki, Deng, Zeyi, Agena, Shinya, Uezato, Jin, Kondo, Shunsuke, Kiyuna, Asanori, Maeda, Hiroyuki, Suzuki, Mikio, Ganaha, Akira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411679/
https://www.ncbi.nlm.nih.gov/pubmed/30594962
http://dx.doi.org/10.1007/s00405-018-05263-x
_version_ 1783402429177397248
author Yamashita, Yukashi
Ikegami, Taro
Hirakawa, Hitoshi
Uehara, Takayuki
Deng, Zeyi
Agena, Shinya
Uezato, Jin
Kondo, Shunsuke
Kiyuna, Asanori
Maeda, Hiroyuki
Suzuki, Mikio
Ganaha, Akira
author_facet Yamashita, Yukashi
Ikegami, Taro
Hirakawa, Hitoshi
Uehara, Takayuki
Deng, Zeyi
Agena, Shinya
Uezato, Jin
Kondo, Shunsuke
Kiyuna, Asanori
Maeda, Hiroyuki
Suzuki, Mikio
Ganaha, Akira
author_sort Yamashita, Yukashi
collection PubMed
description PURPOSE: The aim of this study was to evaluate the 8th edition of the American Joint Committee on Cancer Staging Manual: Head and Neck Section on oropharyngeal squamous cell cancer (OPSCC) and to clarify the relationship between p16 overexpression and the presence of human papillomavirus (HPV) DNA using fresh frozen samples. METHODS: Samples from 100 OPSCC patients were analyzed using polymerase chain reaction (PCR) and p16 immunohistochemistry. RESULTS: Five-year overall survival (OS) was 73.0%, 93.9%, and 62.2% in all, p16-positive (n = 34), and p16-negative (n = 66) cases, respectively. OS tended to be better aligned with stage in the 8th edition than in the 7th edition. The 5-year OS was 96.0% in never or light smokers (< 40 pack-years), and 87.5% in heavy smokers (≥ 40 pack-years) in the p16-positive group, respectively (p = 0.027). HPV infection was found in 100% of p16-positive and 21.2% of p16-negative cases. The p16-positive cases had higher viral load and integrated physical status than the p16-negative cases. Although 1 case with p16 overexpression showed no PCR amplification using consensus primers, PCR amplification was detected using HPV 16 E6-specific primers. CONCLUSIONS: The 8th edition predicts OPSCC prognosis more accurately than the 7th edition and p16-overexpression is an excellent surrogate marker for detecting HPV infection. Although high-risk-type HPV infection was observed in p16-negative cases, it showed no significant effect in survival outcome.
format Online
Article
Text
id pubmed-6411679
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-64116792019-04-03 Staging and prognosis of oropharyngeal carcinoma according to the 8th Edition of the American Joint Committee on Cancer Staging Manual in human papillomavirus infection Yamashita, Yukashi Ikegami, Taro Hirakawa, Hitoshi Uehara, Takayuki Deng, Zeyi Agena, Shinya Uezato, Jin Kondo, Shunsuke Kiyuna, Asanori Maeda, Hiroyuki Suzuki, Mikio Ganaha, Akira Eur Arch Otorhinolaryngol Head & Neck PURPOSE: The aim of this study was to evaluate the 8th edition of the American Joint Committee on Cancer Staging Manual: Head and Neck Section on oropharyngeal squamous cell cancer (OPSCC) and to clarify the relationship between p16 overexpression and the presence of human papillomavirus (HPV) DNA using fresh frozen samples. METHODS: Samples from 100 OPSCC patients were analyzed using polymerase chain reaction (PCR) and p16 immunohistochemistry. RESULTS: Five-year overall survival (OS) was 73.0%, 93.9%, and 62.2% in all, p16-positive (n = 34), and p16-negative (n = 66) cases, respectively. OS tended to be better aligned with stage in the 8th edition than in the 7th edition. The 5-year OS was 96.0% in never or light smokers (< 40 pack-years), and 87.5% in heavy smokers (≥ 40 pack-years) in the p16-positive group, respectively (p = 0.027). HPV infection was found in 100% of p16-positive and 21.2% of p16-negative cases. The p16-positive cases had higher viral load and integrated physical status than the p16-negative cases. Although 1 case with p16 overexpression showed no PCR amplification using consensus primers, PCR amplification was detected using HPV 16 E6-specific primers. CONCLUSIONS: The 8th edition predicts OPSCC prognosis more accurately than the 7th edition and p16-overexpression is an excellent surrogate marker for detecting HPV infection. Although high-risk-type HPV infection was observed in p16-negative cases, it showed no significant effect in survival outcome. Springer Berlin Heidelberg 2018-12-29 2019 /pmc/articles/PMC6411679/ /pubmed/30594962 http://dx.doi.org/10.1007/s00405-018-05263-x Text en © The Author(s) 2018 OpenAccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Head & Neck
Yamashita, Yukashi
Ikegami, Taro
Hirakawa, Hitoshi
Uehara, Takayuki
Deng, Zeyi
Agena, Shinya
Uezato, Jin
Kondo, Shunsuke
Kiyuna, Asanori
Maeda, Hiroyuki
Suzuki, Mikio
Ganaha, Akira
Staging and prognosis of oropharyngeal carcinoma according to the 8th Edition of the American Joint Committee on Cancer Staging Manual in human papillomavirus infection
title Staging and prognosis of oropharyngeal carcinoma according to the 8th Edition of the American Joint Committee on Cancer Staging Manual in human papillomavirus infection
title_full Staging and prognosis of oropharyngeal carcinoma according to the 8th Edition of the American Joint Committee on Cancer Staging Manual in human papillomavirus infection
title_fullStr Staging and prognosis of oropharyngeal carcinoma according to the 8th Edition of the American Joint Committee on Cancer Staging Manual in human papillomavirus infection
title_full_unstemmed Staging and prognosis of oropharyngeal carcinoma according to the 8th Edition of the American Joint Committee on Cancer Staging Manual in human papillomavirus infection
title_short Staging and prognosis of oropharyngeal carcinoma according to the 8th Edition of the American Joint Committee on Cancer Staging Manual in human papillomavirus infection
title_sort staging and prognosis of oropharyngeal carcinoma according to the 8th edition of the american joint committee on cancer staging manual in human papillomavirus infection
topic Head & Neck
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411679/
https://www.ncbi.nlm.nih.gov/pubmed/30594962
http://dx.doi.org/10.1007/s00405-018-05263-x
work_keys_str_mv AT yamashitayukashi stagingandprognosisoforopharyngealcarcinomaaccordingtothe8theditionoftheamericanjointcommitteeoncancerstagingmanualinhumanpapillomavirusinfection
AT ikegamitaro stagingandprognosisoforopharyngealcarcinomaaccordingtothe8theditionoftheamericanjointcommitteeoncancerstagingmanualinhumanpapillomavirusinfection
AT hirakawahitoshi stagingandprognosisoforopharyngealcarcinomaaccordingtothe8theditionoftheamericanjointcommitteeoncancerstagingmanualinhumanpapillomavirusinfection
AT ueharatakayuki stagingandprognosisoforopharyngealcarcinomaaccordingtothe8theditionoftheamericanjointcommitteeoncancerstagingmanualinhumanpapillomavirusinfection
AT dengzeyi stagingandprognosisoforopharyngealcarcinomaaccordingtothe8theditionoftheamericanjointcommitteeoncancerstagingmanualinhumanpapillomavirusinfection
AT agenashinya stagingandprognosisoforopharyngealcarcinomaaccordingtothe8theditionoftheamericanjointcommitteeoncancerstagingmanualinhumanpapillomavirusinfection
AT uezatojin stagingandprognosisoforopharyngealcarcinomaaccordingtothe8theditionoftheamericanjointcommitteeoncancerstagingmanualinhumanpapillomavirusinfection
AT kondoshunsuke stagingandprognosisoforopharyngealcarcinomaaccordingtothe8theditionoftheamericanjointcommitteeoncancerstagingmanualinhumanpapillomavirusinfection
AT kiyunaasanori stagingandprognosisoforopharyngealcarcinomaaccordingtothe8theditionoftheamericanjointcommitteeoncancerstagingmanualinhumanpapillomavirusinfection
AT maedahiroyuki stagingandprognosisoforopharyngealcarcinomaaccordingtothe8theditionoftheamericanjointcommitteeoncancerstagingmanualinhumanpapillomavirusinfection
AT suzukimikio stagingandprognosisoforopharyngealcarcinomaaccordingtothe8theditionoftheamericanjointcommitteeoncancerstagingmanualinhumanpapillomavirusinfection
AT ganahaakira stagingandprognosisoforopharyngealcarcinomaaccordingtothe8theditionoftheamericanjointcommitteeoncancerstagingmanualinhumanpapillomavirusinfection