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Anti-bacterial activity of baicalin against APEC through inhibition of quorum sensing and inflammatory responses
Avian pathogenic Escherichia coli (APEC), collectively known as causative agent of extraintestinal infections, is an important cause of morbidity and mortality in poultry. Currently, quorum sensing (QS), biofilm formation and virulence factors are considered as novel prospective targets for antimicr...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411720/ https://www.ncbi.nlm.nih.gov/pubmed/30858423 http://dx.doi.org/10.1038/s41598-019-40684-6 |
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author | Peng, Lu-Yuan Yuan, Meng Wu, Zong-Mei Song, Ke Zhang, Chun-Lei An, Qiang Xia, Fang Yu, Jia-Lin Yi, Peng-Fei Fu, Ben-Dong Shen, Hai-Qing |
author_facet | Peng, Lu-Yuan Yuan, Meng Wu, Zong-Mei Song, Ke Zhang, Chun-Lei An, Qiang Xia, Fang Yu, Jia-Lin Yi, Peng-Fei Fu, Ben-Dong Shen, Hai-Qing |
author_sort | Peng, Lu-Yuan |
collection | PubMed |
description | Avian pathogenic Escherichia coli (APEC), collectively known as causative agent of extraintestinal infections, is an important cause of morbidity and mortality in poultry. Currently, quorum sensing (QS), biofilm formation and virulence factors are considered as novel prospective targets for antimicrobial therapy to control APEC invasion. In addition, inflammatory responses are also served as the major pathological features of APEC invasion. This study was aimed to explore the effect of baicalin on APEC and APEC-induced inflammatory responses. After treatment with baicalin, we mainly examined the AI-2 secretion, biofilm formation, expression of virulence genes of APEC, and the levels of inflammatory cytokines, as well as the expression of NF-κB pathway. Our results showed that baicalin significantly inhibited the QS via decreasing the AI-2 secretion, biofilm formation, and the expression of virulence genes of APEC such as LsrB, LsrK, LuxS, pfs, H-NS, fimA, fimB, fyuA, csgA, csgB, and rpoS. Moreover, baicalin significantly attenuated the release of lactate dehydrogenase (LDH), and the adhesion of APEC to chicken type II pneumocytes to reduce cell damage. Furthermore, baicalin also inhibited the expression of pro-inflammatory cytokines and NF-κB activation. Thus, our data revealed that baicalin could interfere with the quorum sensing, biofilm formation and virulence genes expression to relieve the APEC pathogenicity. Additionally, baicalin decreased the inflammatory responses of chicken type II pneumocytes induced by APEC. Taken together, these data provide a novel potential pharmaco-therapeutic approach to chicken colibacillosis. |
format | Online Article Text |
id | pubmed-6411720 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-64117202019-03-13 Anti-bacterial activity of baicalin against APEC through inhibition of quorum sensing and inflammatory responses Peng, Lu-Yuan Yuan, Meng Wu, Zong-Mei Song, Ke Zhang, Chun-Lei An, Qiang Xia, Fang Yu, Jia-Lin Yi, Peng-Fei Fu, Ben-Dong Shen, Hai-Qing Sci Rep Article Avian pathogenic Escherichia coli (APEC), collectively known as causative agent of extraintestinal infections, is an important cause of morbidity and mortality in poultry. Currently, quorum sensing (QS), biofilm formation and virulence factors are considered as novel prospective targets for antimicrobial therapy to control APEC invasion. In addition, inflammatory responses are also served as the major pathological features of APEC invasion. This study was aimed to explore the effect of baicalin on APEC and APEC-induced inflammatory responses. After treatment with baicalin, we mainly examined the AI-2 secretion, biofilm formation, expression of virulence genes of APEC, and the levels of inflammatory cytokines, as well as the expression of NF-κB pathway. Our results showed that baicalin significantly inhibited the QS via decreasing the AI-2 secretion, biofilm formation, and the expression of virulence genes of APEC such as LsrB, LsrK, LuxS, pfs, H-NS, fimA, fimB, fyuA, csgA, csgB, and rpoS. Moreover, baicalin significantly attenuated the release of lactate dehydrogenase (LDH), and the adhesion of APEC to chicken type II pneumocytes to reduce cell damage. Furthermore, baicalin also inhibited the expression of pro-inflammatory cytokines and NF-κB activation. Thus, our data revealed that baicalin could interfere with the quorum sensing, biofilm formation and virulence genes expression to relieve the APEC pathogenicity. Additionally, baicalin decreased the inflammatory responses of chicken type II pneumocytes induced by APEC. Taken together, these data provide a novel potential pharmaco-therapeutic approach to chicken colibacillosis. Nature Publishing Group UK 2019-03-11 /pmc/articles/PMC6411720/ /pubmed/30858423 http://dx.doi.org/10.1038/s41598-019-40684-6 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Peng, Lu-Yuan Yuan, Meng Wu, Zong-Mei Song, Ke Zhang, Chun-Lei An, Qiang Xia, Fang Yu, Jia-Lin Yi, Peng-Fei Fu, Ben-Dong Shen, Hai-Qing Anti-bacterial activity of baicalin against APEC through inhibition of quorum sensing and inflammatory responses |
title | Anti-bacterial activity of baicalin against APEC through inhibition of quorum sensing and inflammatory responses |
title_full | Anti-bacterial activity of baicalin against APEC through inhibition of quorum sensing and inflammatory responses |
title_fullStr | Anti-bacterial activity of baicalin against APEC through inhibition of quorum sensing and inflammatory responses |
title_full_unstemmed | Anti-bacterial activity of baicalin against APEC through inhibition of quorum sensing and inflammatory responses |
title_short | Anti-bacterial activity of baicalin against APEC through inhibition of quorum sensing and inflammatory responses |
title_sort | anti-bacterial activity of baicalin against apec through inhibition of quorum sensing and inflammatory responses |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411720/ https://www.ncbi.nlm.nih.gov/pubmed/30858423 http://dx.doi.org/10.1038/s41598-019-40684-6 |
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