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Plasma-activated medium triggers cell death and the presentation of immune activating danger signals in melanoma and pancreatic cancer cells

Over the past decade, cold atmospheric plasmas have shown promising application in cancer therapy. The therapeutic use of plasma-activated media is a topic addressed in an emerging field known as plasma pharmacy. In oncology, plasma-activated media are  used to harness the therapeutic effects of oxi...

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Autores principales: Azzariti, Amalia, Iacobazzi, Rosa Maria, Di Fonte, Roberta, Porcelli, Letizia, Gristina, Roberto, Favia, Pietro, Fracassi, Francesco, Trizio, Ilaria, Silvestris, Nicola, Guida, Gabriella, Tommasi, Stefania, Sardella, Eloisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411873/
https://www.ncbi.nlm.nih.gov/pubmed/30858524
http://dx.doi.org/10.1038/s41598-019-40637-z
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author Azzariti, Amalia
Iacobazzi, Rosa Maria
Di Fonte, Roberta
Porcelli, Letizia
Gristina, Roberto
Favia, Pietro
Fracassi, Francesco
Trizio, Ilaria
Silvestris, Nicola
Guida, Gabriella
Tommasi, Stefania
Sardella, Eloisa
author_facet Azzariti, Amalia
Iacobazzi, Rosa Maria
Di Fonte, Roberta
Porcelli, Letizia
Gristina, Roberto
Favia, Pietro
Fracassi, Francesco
Trizio, Ilaria
Silvestris, Nicola
Guida, Gabriella
Tommasi, Stefania
Sardella, Eloisa
author_sort Azzariti, Amalia
collection PubMed
description Over the past decade, cold atmospheric plasmas have shown promising application in cancer therapy. The therapeutic use of plasma-activated media is a topic addressed in an emerging field known as plasma pharmacy. In oncology, plasma-activated media are  used to harness the therapeutic effects of oxidant species when they come in contact with cancer cells. Among several factors that contribute to the anticancer effect of plasma-activated liquid media (PALM), H(2)O(2) and NO derivatives likely play a key role in the apoptotic pathway. Despite the significant amount of literature produced in recent years, a full understanding of the mechanisms by which PALM exert their activity against cancer cells is limited. In this paper, a sealed dielectric-barrier discharge was used to disentangle the effect of reactive nitrogen species (RNS) from that of reactive oxygen species (ROS) on cancer cells. Two cancers characterized by poor prognosis have been investigated: metastatic melanoma and pancreatic cancer. Both tumour models exposed to PALM rich in H(2)O(2) showed a reduction in proliferation and an increase in calreticulin exposure and ATP release, suggesting the potential use of activated media as an inducer of immunogenic cell death via activation of the innate immune system.
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spelling pubmed-64118732019-03-13 Plasma-activated medium triggers cell death and the presentation of immune activating danger signals in melanoma and pancreatic cancer cells Azzariti, Amalia Iacobazzi, Rosa Maria Di Fonte, Roberta Porcelli, Letizia Gristina, Roberto Favia, Pietro Fracassi, Francesco Trizio, Ilaria Silvestris, Nicola Guida, Gabriella Tommasi, Stefania Sardella, Eloisa Sci Rep Article Over the past decade, cold atmospheric plasmas have shown promising application in cancer therapy. The therapeutic use of plasma-activated media is a topic addressed in an emerging field known as plasma pharmacy. In oncology, plasma-activated media are  used to harness the therapeutic effects of oxidant species when they come in contact with cancer cells. Among several factors that contribute to the anticancer effect of plasma-activated liquid media (PALM), H(2)O(2) and NO derivatives likely play a key role in the apoptotic pathway. Despite the significant amount of literature produced in recent years, a full understanding of the mechanisms by which PALM exert their activity against cancer cells is limited. In this paper, a sealed dielectric-barrier discharge was used to disentangle the effect of reactive nitrogen species (RNS) from that of reactive oxygen species (ROS) on cancer cells. Two cancers characterized by poor prognosis have been investigated: metastatic melanoma and pancreatic cancer. Both tumour models exposed to PALM rich in H(2)O(2) showed a reduction in proliferation and an increase in calreticulin exposure and ATP release, suggesting the potential use of activated media as an inducer of immunogenic cell death via activation of the innate immune system. Nature Publishing Group UK 2019-03-11 /pmc/articles/PMC6411873/ /pubmed/30858524 http://dx.doi.org/10.1038/s41598-019-40637-z Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Azzariti, Amalia
Iacobazzi, Rosa Maria
Di Fonte, Roberta
Porcelli, Letizia
Gristina, Roberto
Favia, Pietro
Fracassi, Francesco
Trizio, Ilaria
Silvestris, Nicola
Guida, Gabriella
Tommasi, Stefania
Sardella, Eloisa
Plasma-activated medium triggers cell death and the presentation of immune activating danger signals in melanoma and pancreatic cancer cells
title Plasma-activated medium triggers cell death and the presentation of immune activating danger signals in melanoma and pancreatic cancer cells
title_full Plasma-activated medium triggers cell death and the presentation of immune activating danger signals in melanoma and pancreatic cancer cells
title_fullStr Plasma-activated medium triggers cell death and the presentation of immune activating danger signals in melanoma and pancreatic cancer cells
title_full_unstemmed Plasma-activated medium triggers cell death and the presentation of immune activating danger signals in melanoma and pancreatic cancer cells
title_short Plasma-activated medium triggers cell death and the presentation of immune activating danger signals in melanoma and pancreatic cancer cells
title_sort plasma-activated medium triggers cell death and the presentation of immune activating danger signals in melanoma and pancreatic cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411873/
https://www.ncbi.nlm.nih.gov/pubmed/30858524
http://dx.doi.org/10.1038/s41598-019-40637-z
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