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Preparation of kenaf stem hemicellulosic hydrolysate and its fermentability in microbial production of xylitol by Escherichia coliBL21

Kenaf (Hibiscus cannabinus L.), a potential fibre crop with a desirably high growth rate, could serve as a sustainable feedstock in the production of xylitol. In this work, the extraction of soluble products of kenaf through dilute nitric-acid hydrolysis was elucidated with respect to three paramete...

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Autores principales: Shah, Siti Syazwani Mohd, Luthfi, Abdullah Amru Indera, Low, Kheng Oon, Harun, Shuhaida, Manaf, Shareena Fairuz Abdul, Illias, Rosli Md., Jahim, Jamaliah Md.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411968/
https://www.ncbi.nlm.nih.gov/pubmed/30858467
http://dx.doi.org/10.1038/s41598-019-40807-z
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author Shah, Siti Syazwani Mohd
Luthfi, Abdullah Amru Indera
Low, Kheng Oon
Harun, Shuhaida
Manaf, Shareena Fairuz Abdul
Illias, Rosli Md.
Jahim, Jamaliah Md.
author_facet Shah, Siti Syazwani Mohd
Luthfi, Abdullah Amru Indera
Low, Kheng Oon
Harun, Shuhaida
Manaf, Shareena Fairuz Abdul
Illias, Rosli Md.
Jahim, Jamaliah Md.
author_sort Shah, Siti Syazwani Mohd
collection PubMed
description Kenaf (Hibiscus cannabinus L.), a potential fibre crop with a desirably high growth rate, could serve as a sustainable feedstock in the production of xylitol. In this work, the extraction of soluble products of kenaf through dilute nitric-acid hydrolysis was elucidated with respect to three parameters, namely temperature, residence time, and acid concentration. The study will assist in evaluating the performance in terms of xylose recovery. The result point out that the maximum xylose yield of 30.7 g per 100 g of dry kenaf was attained from 2% (v/v) HNO(3) at 130 °C for 60 min. The detoxified hydrolysate was incorporated as the primary carbon source for subsequent fermentation by recombinant Escherichia coli and the performance of strain on five different semi-synthetic media on xylitol production were evaluated herein. Among these media, batch cultivation in a basal salt medium (BSM) afforded the highest xylitol yield of 0.35 g/g based on xylose consumption, which corresponded to 92.8% substrate utilization after 38 h. Subsequently, fermentation by E. coli in the xylose-based kenaf hydrolysate supplemented with BSM resulting in 6.8 g/L xylitol which corresponding to xylitol yield of 0.38 g/g. These findings suggested that the use of kenaf as the fermentation feedstock could be advantageous for the development of sustainable xylitol production.
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spelling pubmed-64119682019-03-13 Preparation of kenaf stem hemicellulosic hydrolysate and its fermentability in microbial production of xylitol by Escherichia coliBL21 Shah, Siti Syazwani Mohd Luthfi, Abdullah Amru Indera Low, Kheng Oon Harun, Shuhaida Manaf, Shareena Fairuz Abdul Illias, Rosli Md. Jahim, Jamaliah Md. Sci Rep Article Kenaf (Hibiscus cannabinus L.), a potential fibre crop with a desirably high growth rate, could serve as a sustainable feedstock in the production of xylitol. In this work, the extraction of soluble products of kenaf through dilute nitric-acid hydrolysis was elucidated with respect to three parameters, namely temperature, residence time, and acid concentration. The study will assist in evaluating the performance in terms of xylose recovery. The result point out that the maximum xylose yield of 30.7 g per 100 g of dry kenaf was attained from 2% (v/v) HNO(3) at 130 °C for 60 min. The detoxified hydrolysate was incorporated as the primary carbon source for subsequent fermentation by recombinant Escherichia coli and the performance of strain on five different semi-synthetic media on xylitol production were evaluated herein. Among these media, batch cultivation in a basal salt medium (BSM) afforded the highest xylitol yield of 0.35 g/g based on xylose consumption, which corresponded to 92.8% substrate utilization after 38 h. Subsequently, fermentation by E. coli in the xylose-based kenaf hydrolysate supplemented with BSM resulting in 6.8 g/L xylitol which corresponding to xylitol yield of 0.38 g/g. These findings suggested that the use of kenaf as the fermentation feedstock could be advantageous for the development of sustainable xylitol production. Nature Publishing Group UK 2019-03-11 /pmc/articles/PMC6411968/ /pubmed/30858467 http://dx.doi.org/10.1038/s41598-019-40807-z Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Shah, Siti Syazwani Mohd
Luthfi, Abdullah Amru Indera
Low, Kheng Oon
Harun, Shuhaida
Manaf, Shareena Fairuz Abdul
Illias, Rosli Md.
Jahim, Jamaliah Md.
Preparation of kenaf stem hemicellulosic hydrolysate and its fermentability in microbial production of xylitol by Escherichia coliBL21
title Preparation of kenaf stem hemicellulosic hydrolysate and its fermentability in microbial production of xylitol by Escherichia coliBL21
title_full Preparation of kenaf stem hemicellulosic hydrolysate and its fermentability in microbial production of xylitol by Escherichia coliBL21
title_fullStr Preparation of kenaf stem hemicellulosic hydrolysate and its fermentability in microbial production of xylitol by Escherichia coliBL21
title_full_unstemmed Preparation of kenaf stem hemicellulosic hydrolysate and its fermentability in microbial production of xylitol by Escherichia coliBL21
title_short Preparation of kenaf stem hemicellulosic hydrolysate and its fermentability in microbial production of xylitol by Escherichia coliBL21
title_sort preparation of kenaf stem hemicellulosic hydrolysate and its fermentability in microbial production of xylitol by escherichia colibl21
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411968/
https://www.ncbi.nlm.nih.gov/pubmed/30858467
http://dx.doi.org/10.1038/s41598-019-40807-z
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