Cargando…

Where the plasmids roam: large-scale sequence analysis reveals plasmids with large host ranges

Describing the role of plasmids and their contribution to the exchange of genetic material among bacteria is essential for understanding the fields of plasmid epidemiology, microbial ecology, and commercial and synthetic microbiology. Broad-host-range (BHR) plasmids are those that are found not only...

Descripción completa

Detalles Bibliográficos
Autores principales: Brooks, Lauren Elisabeth, Kaze, Mo, Sistrom, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412061/
https://www.ncbi.nlm.nih.gov/pubmed/30625112
http://dx.doi.org/10.1099/mgen.0.000244
_version_ 1783402517590179840
author Brooks, Lauren Elisabeth
Kaze, Mo
Sistrom, Mark
author_facet Brooks, Lauren Elisabeth
Kaze, Mo
Sistrom, Mark
author_sort Brooks, Lauren Elisabeth
collection PubMed
description Describing the role of plasmids and their contribution to the exchange of genetic material among bacteria is essential for understanding the fields of plasmid epidemiology, microbial ecology, and commercial and synthetic microbiology. Broad-host-range (BHR) plasmids are those that are found not only in a single bacterial species, but in members of different taxonomic groups and are of significant interest to researchers in many fields. We applied a novel approach to computationally identify new BHR plasmids, in which we searched for highly similar cognate plasmids within a comprehensive plasmid database. After identifying 125 plasmid groups with highly similar cognates found in multiple taxa, we closely examined BHR plasmids found in multiple families. The majority of our identified BHR plasmids are found in members of the Enterobacteriaceae and closely related taxa, while three BHR plasmids of potential commercial significance were found in two species of Cyanobacteria. One plasmid with an exceptionally broad host range was found in both Gram-positive and Gram-negative bacterial species. This analysis demonstrates the utility of this method in identifying new BHR plasmids while highlighting unknown ranges of previously documented plasmids.
format Online
Article
Text
id pubmed-6412061
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Microbiology Society
record_format MEDLINE/PubMed
spelling pubmed-64120612019-03-12 Where the plasmids roam: large-scale sequence analysis reveals plasmids with large host ranges Brooks, Lauren Elisabeth Kaze, Mo Sistrom, Mark Microb Genom Research Article Describing the role of plasmids and their contribution to the exchange of genetic material among bacteria is essential for understanding the fields of plasmid epidemiology, microbial ecology, and commercial and synthetic microbiology. Broad-host-range (BHR) plasmids are those that are found not only in a single bacterial species, but in members of different taxonomic groups and are of significant interest to researchers in many fields. We applied a novel approach to computationally identify new BHR plasmids, in which we searched for highly similar cognate plasmids within a comprehensive plasmid database. After identifying 125 plasmid groups with highly similar cognates found in multiple taxa, we closely examined BHR plasmids found in multiple families. The majority of our identified BHR plasmids are found in members of the Enterobacteriaceae and closely related taxa, while three BHR plasmids of potential commercial significance were found in two species of Cyanobacteria. One plasmid with an exceptionally broad host range was found in both Gram-positive and Gram-negative bacterial species. This analysis demonstrates the utility of this method in identifying new BHR plasmids while highlighting unknown ranges of previously documented plasmids. Microbiology Society 2019-01-09 /pmc/articles/PMC6412061/ /pubmed/30625112 http://dx.doi.org/10.1099/mgen.0.000244 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Brooks, Lauren Elisabeth
Kaze, Mo
Sistrom, Mark
Where the plasmids roam: large-scale sequence analysis reveals plasmids with large host ranges
title Where the plasmids roam: large-scale sequence analysis reveals plasmids with large host ranges
title_full Where the plasmids roam: large-scale sequence analysis reveals plasmids with large host ranges
title_fullStr Where the plasmids roam: large-scale sequence analysis reveals plasmids with large host ranges
title_full_unstemmed Where the plasmids roam: large-scale sequence analysis reveals plasmids with large host ranges
title_short Where the plasmids roam: large-scale sequence analysis reveals plasmids with large host ranges
title_sort where the plasmids roam: large-scale sequence analysis reveals plasmids with large host ranges
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412061/
https://www.ncbi.nlm.nih.gov/pubmed/30625112
http://dx.doi.org/10.1099/mgen.0.000244
work_keys_str_mv AT brookslaurenelisabeth wheretheplasmidsroamlargescalesequenceanalysisrevealsplasmidswithlargehostranges
AT kazemo wheretheplasmidsroamlargescalesequenceanalysisrevealsplasmidswithlargehostranges
AT sistrommark wheretheplasmidsroamlargescalesequenceanalysisrevealsplasmidswithlargehostranges