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Selection of self-priming molecular replicators

Self-priming amplification of oligonucleotides is possible based on foldback of 3′ ends, self-priming, and concatemerization, especially in the presence of phosphorothioate linkages. Such a simple replicative mechanism may have led to the accumulation of specific replicators at or near the origin of...

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Detalles Bibliográficos
Autores principales: Park, Daechan, Ellington, Andrew D, Jung, Cheulhee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412129/
https://www.ncbi.nlm.nih.gov/pubmed/30698805
http://dx.doi.org/10.1093/nar/gkz044
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author Park, Daechan
Ellington, Andrew D
Jung, Cheulhee
author_facet Park, Daechan
Ellington, Andrew D
Jung, Cheulhee
author_sort Park, Daechan
collection PubMed
description Self-priming amplification of oligonucleotides is possible based on foldback of 3′ ends, self-priming, and concatemerization, especially in the presence of phosphorothioate linkages. Such a simple replicative mechanism may have led to the accumulation of specific replicators at or near the origin of life. To determine how early replicators may have competed with one another, we have carried out selections with phosphorothiolated hairpins appended to a short random sequence library (N10). Upon the addition of deoxynucleoside triphosphates and a polymerase, concatemers quickly formed, and those random sequences that templated the insertion of purines, especially during initiation, quickly predominated. Over several serial transfers, particular sequences accumulated, and in isolation these were shown to outcompete less efficient replicators.
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spelling pubmed-64121292019-03-18 Selection of self-priming molecular replicators Park, Daechan Ellington, Andrew D Jung, Cheulhee Nucleic Acids Res Chemical Biology and Nucleic Acid Chemistry Self-priming amplification of oligonucleotides is possible based on foldback of 3′ ends, self-priming, and concatemerization, especially in the presence of phosphorothioate linkages. Such a simple replicative mechanism may have led to the accumulation of specific replicators at or near the origin of life. To determine how early replicators may have competed with one another, we have carried out selections with phosphorothiolated hairpins appended to a short random sequence library (N10). Upon the addition of deoxynucleoside triphosphates and a polymerase, concatemers quickly formed, and those random sequences that templated the insertion of purines, especially during initiation, quickly predominated. Over several serial transfers, particular sequences accumulated, and in isolation these were shown to outcompete less efficient replicators. Oxford University Press 2019-03-18 2019-01-30 /pmc/articles/PMC6412129/ /pubmed/30698805 http://dx.doi.org/10.1093/nar/gkz044 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Chemical Biology and Nucleic Acid Chemistry
Park, Daechan
Ellington, Andrew D
Jung, Cheulhee
Selection of self-priming molecular replicators
title Selection of self-priming molecular replicators
title_full Selection of self-priming molecular replicators
title_fullStr Selection of self-priming molecular replicators
title_full_unstemmed Selection of self-priming molecular replicators
title_short Selection of self-priming molecular replicators
title_sort selection of self-priming molecular replicators
topic Chemical Biology and Nucleic Acid Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412129/
https://www.ncbi.nlm.nih.gov/pubmed/30698805
http://dx.doi.org/10.1093/nar/gkz044
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