Cargando…

Genome-Wide Characterization of NBS-Encoding Genes in Watermelon and Their Potential Association with Gummy Stem Blight Resistance

Watermelon (Citrullus lanatus) is a nutritionally rich and economically important horticultural crop of the Cucurbitaceae family. Gummy stem blight (GSB) is a major disease of watermelon, which is caused by the fungus Didymella bryoniae, and results in substantial economic losses in terms of yield a...

Descripción completa

Detalles Bibliográficos
Autores principales: Hassan, Md Zahid, Rahim, Md Abdur, Jung, Hee-Jeong, Park, Jong-In, Kim, Hoy-Taek, Nou, Ill-Sup
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412240/
https://www.ncbi.nlm.nih.gov/pubmed/30791419
http://dx.doi.org/10.3390/ijms20040902
_version_ 1783402559302533120
author Hassan, Md Zahid
Rahim, Md Abdur
Jung, Hee-Jeong
Park, Jong-In
Kim, Hoy-Taek
Nou, Ill-Sup
author_facet Hassan, Md Zahid
Rahim, Md Abdur
Jung, Hee-Jeong
Park, Jong-In
Kim, Hoy-Taek
Nou, Ill-Sup
author_sort Hassan, Md Zahid
collection PubMed
description Watermelon (Citrullus lanatus) is a nutritionally rich and economically important horticultural crop of the Cucurbitaceae family. Gummy stem blight (GSB) is a major disease of watermelon, which is caused by the fungus Didymella bryoniae, and results in substantial economic losses in terms of yield and quality. However, only a few molecular studies have focused on GSB resistance in watermelon. Nucleotide binding site (NBS)-encoding resistance (R) genes play important roles in plant defense responses to several pathogens, but little is known about the role of NBS-encoding genes in disease resistance in watermelon. The analyzed NBS-encoding R genes comprises several domains, including Toll/interleukin-1 receptor(TIR), NBS, leucine-rich repeat (LRR), resistance to powdery mildew8(RPW8) and coiled coil (CC), which are known to be involved in disease resistance. We determined the expression patterns of these R genes in resistant and susceptible watermelon lines at different time points after D. bryoniae infection by quantitative RT-PCR. The R genes exhibited various expression patterns in the resistant watermelon compared to the susceptible watermelon. Only six R genes exhibited consistent expression patterns (Cla001821, Cla019863, Cla020705, Cla012430, Cla012433 and Cla012439), which were higher in the resistant line compared to the susceptible line. Our study provides fundamental insights into the NBS-LRR gene family in watermelon in response to D. bryoniae infection. Further functional studies of these six candidate resistance genes should help to advance breeding programs aimed at improving disease resistance in watermelons.
format Online
Article
Text
id pubmed-6412240
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-64122402019-04-05 Genome-Wide Characterization of NBS-Encoding Genes in Watermelon and Their Potential Association with Gummy Stem Blight Resistance Hassan, Md Zahid Rahim, Md Abdur Jung, Hee-Jeong Park, Jong-In Kim, Hoy-Taek Nou, Ill-Sup Int J Mol Sci Article Watermelon (Citrullus lanatus) is a nutritionally rich and economically important horticultural crop of the Cucurbitaceae family. Gummy stem blight (GSB) is a major disease of watermelon, which is caused by the fungus Didymella bryoniae, and results in substantial economic losses in terms of yield and quality. However, only a few molecular studies have focused on GSB resistance in watermelon. Nucleotide binding site (NBS)-encoding resistance (R) genes play important roles in plant defense responses to several pathogens, but little is known about the role of NBS-encoding genes in disease resistance in watermelon. The analyzed NBS-encoding R genes comprises several domains, including Toll/interleukin-1 receptor(TIR), NBS, leucine-rich repeat (LRR), resistance to powdery mildew8(RPW8) and coiled coil (CC), which are known to be involved in disease resistance. We determined the expression patterns of these R genes in resistant and susceptible watermelon lines at different time points after D. bryoniae infection by quantitative RT-PCR. The R genes exhibited various expression patterns in the resistant watermelon compared to the susceptible watermelon. Only six R genes exhibited consistent expression patterns (Cla001821, Cla019863, Cla020705, Cla012430, Cla012433 and Cla012439), which were higher in the resistant line compared to the susceptible line. Our study provides fundamental insights into the NBS-LRR gene family in watermelon in response to D. bryoniae infection. Further functional studies of these six candidate resistance genes should help to advance breeding programs aimed at improving disease resistance in watermelons. MDPI 2019-02-19 /pmc/articles/PMC6412240/ /pubmed/30791419 http://dx.doi.org/10.3390/ijms20040902 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hassan, Md Zahid
Rahim, Md Abdur
Jung, Hee-Jeong
Park, Jong-In
Kim, Hoy-Taek
Nou, Ill-Sup
Genome-Wide Characterization of NBS-Encoding Genes in Watermelon and Their Potential Association with Gummy Stem Blight Resistance
title Genome-Wide Characterization of NBS-Encoding Genes in Watermelon and Their Potential Association with Gummy Stem Blight Resistance
title_full Genome-Wide Characterization of NBS-Encoding Genes in Watermelon and Their Potential Association with Gummy Stem Blight Resistance
title_fullStr Genome-Wide Characterization of NBS-Encoding Genes in Watermelon and Their Potential Association with Gummy Stem Blight Resistance
title_full_unstemmed Genome-Wide Characterization of NBS-Encoding Genes in Watermelon and Their Potential Association with Gummy Stem Blight Resistance
title_short Genome-Wide Characterization of NBS-Encoding Genes in Watermelon and Their Potential Association with Gummy Stem Blight Resistance
title_sort genome-wide characterization of nbs-encoding genes in watermelon and their potential association with gummy stem blight resistance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412240/
https://www.ncbi.nlm.nih.gov/pubmed/30791419
http://dx.doi.org/10.3390/ijms20040902
work_keys_str_mv AT hassanmdzahid genomewidecharacterizationofnbsencodinggenesinwatermelonandtheirpotentialassociationwithgummystemblightresistance
AT rahimmdabdur genomewidecharacterizationofnbsencodinggenesinwatermelonandtheirpotentialassociationwithgummystemblightresistance
AT jungheejeong genomewidecharacterizationofnbsencodinggenesinwatermelonandtheirpotentialassociationwithgummystemblightresistance
AT parkjongin genomewidecharacterizationofnbsencodinggenesinwatermelonandtheirpotentialassociationwithgummystemblightresistance
AT kimhoytaek genomewidecharacterizationofnbsencodinggenesinwatermelonandtheirpotentialassociationwithgummystemblightresistance
AT nouillsup genomewidecharacterizationofnbsencodinggenesinwatermelonandtheirpotentialassociationwithgummystemblightresistance