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Antihyperlipidemic Effect, Identification and Isolation of the Lipophilic Components from Artemisia integrifolia
Artemisia integrifolia L. (Compositae) is a medicinal and edible plant. To investigate its antihyperlipidemic effect, a crude lipophilic extract and the composing compounds were isolated and fractioned from the petroleum ether extract of aerial parts of A. integrifolia using column chromatography on...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412335/ https://www.ncbi.nlm.nih.gov/pubmed/30781592 http://dx.doi.org/10.3390/molecules24040725 |
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author | Xu, Yanhua Wang, Qinghu Bao, Wenqiang Pa, Biligetu |
author_facet | Xu, Yanhua Wang, Qinghu Bao, Wenqiang Pa, Biligetu |
author_sort | Xu, Yanhua |
collection | PubMed |
description | Artemisia integrifolia L. (Compositae) is a medicinal and edible plant. To investigate its antihyperlipidemic effect, a crude lipophilic extract and the composing compounds were isolated and fractioned from the petroleum ether extract of aerial parts of A. integrifolia using column chromatography on silica gel. The anti-hyperlipidemia effect was studied in a rat model of acute hyperlipidemia, which was induced by triton WR-1339. A new compound, integrinol (4), together with nine known compounds, namely chamazulene (1), acetylenes (E)-2 (2), acetylenes (E)-3 (3), eugenol (5), palmitic acid (6), oleic acid (7), linoleic acid (8), linolenic acid (9) and 12,13-epoxylinolenic acid were isolated from the crude lipophilic extract of A. integrifolia. The LD50 value of the crude extract was more than 4 g/kg. In Triton WR-1339-induced acute hyperlipidemia model, the crude lipophilic extract (200 mg/kg) significantly reduced total cholesterol (TC) by 70% (p ≤ 0.01) and triglycerides (TGs) by 94% (p ≤ 0.001). The fractioned compounds, such as chamazulene (1), acetylene-2 (2), and linolenic acid (9), used at 4 mg/kg dose, also significantly decreased the concentrations of TC (32%, 33% and 64%, respectively) and TGs (48%, 33% and 93%, respectively). These compounds (i.e., chamazulene, acetylenes (E)-2, and linolenic acid) were considered to be responsible for the bioactive antihyperlipidemic effect. In conclusion, the crude lipid extract of Artemisia integrifolia L. could be used as a potential treatment to avert hyperlipidemia. Further studies to confirm these results in other models of hyperlipidemia (e.g., diet-induced obesity) are warranted. |
format | Online Article Text |
id | pubmed-6412335 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64123352019-04-09 Antihyperlipidemic Effect, Identification and Isolation of the Lipophilic Components from Artemisia integrifolia Xu, Yanhua Wang, Qinghu Bao, Wenqiang Pa, Biligetu Molecules Article Artemisia integrifolia L. (Compositae) is a medicinal and edible plant. To investigate its antihyperlipidemic effect, a crude lipophilic extract and the composing compounds were isolated and fractioned from the petroleum ether extract of aerial parts of A. integrifolia using column chromatography on silica gel. The anti-hyperlipidemia effect was studied in a rat model of acute hyperlipidemia, which was induced by triton WR-1339. A new compound, integrinol (4), together with nine known compounds, namely chamazulene (1), acetylenes (E)-2 (2), acetylenes (E)-3 (3), eugenol (5), palmitic acid (6), oleic acid (7), linoleic acid (8), linolenic acid (9) and 12,13-epoxylinolenic acid were isolated from the crude lipophilic extract of A. integrifolia. The LD50 value of the crude extract was more than 4 g/kg. In Triton WR-1339-induced acute hyperlipidemia model, the crude lipophilic extract (200 mg/kg) significantly reduced total cholesterol (TC) by 70% (p ≤ 0.01) and triglycerides (TGs) by 94% (p ≤ 0.001). The fractioned compounds, such as chamazulene (1), acetylene-2 (2), and linolenic acid (9), used at 4 mg/kg dose, also significantly decreased the concentrations of TC (32%, 33% and 64%, respectively) and TGs (48%, 33% and 93%, respectively). These compounds (i.e., chamazulene, acetylenes (E)-2, and linolenic acid) were considered to be responsible for the bioactive antihyperlipidemic effect. In conclusion, the crude lipid extract of Artemisia integrifolia L. could be used as a potential treatment to avert hyperlipidemia. Further studies to confirm these results in other models of hyperlipidemia (e.g., diet-induced obesity) are warranted. MDPI 2019-02-17 /pmc/articles/PMC6412335/ /pubmed/30781592 http://dx.doi.org/10.3390/molecules24040725 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xu, Yanhua Wang, Qinghu Bao, Wenqiang Pa, Biligetu Antihyperlipidemic Effect, Identification and Isolation of the Lipophilic Components from Artemisia integrifolia |
title | Antihyperlipidemic Effect, Identification and Isolation of the Lipophilic Components from Artemisia integrifolia |
title_full | Antihyperlipidemic Effect, Identification and Isolation of the Lipophilic Components from Artemisia integrifolia |
title_fullStr | Antihyperlipidemic Effect, Identification and Isolation of the Lipophilic Components from Artemisia integrifolia |
title_full_unstemmed | Antihyperlipidemic Effect, Identification and Isolation of the Lipophilic Components from Artemisia integrifolia |
title_short | Antihyperlipidemic Effect, Identification and Isolation of the Lipophilic Components from Artemisia integrifolia |
title_sort | antihyperlipidemic effect, identification and isolation of the lipophilic components from artemisia integrifolia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412335/ https://www.ncbi.nlm.nih.gov/pubmed/30781592 http://dx.doi.org/10.3390/molecules24040725 |
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