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Divergent Effectiveness of Multispecies Probiotic Preparations on Intestinal Microbiota Structure Depends on Metabolic Properties

A growing body of evidence suggests that probiotic functionality is not accurately predicted by their taxonomy. Here, we have set up a study to investigate the effectiveness of two probiotic formulations containing a blend of seven bacterial species in modulating intestinal inflammation in two roden...

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Autores principales: Biagioli, Michele, Capobianco, Daniela, Carino, Adriana, Marchianò, Silvia, Fiorucci, Chiara, Ricci, Patrizia, Distrutti, Eleonora, Fiorucci, Stefano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412585/
https://www.ncbi.nlm.nih.gov/pubmed/30717413
http://dx.doi.org/10.3390/nu11020325
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author Biagioli, Michele
Capobianco, Daniela
Carino, Adriana
Marchianò, Silvia
Fiorucci, Chiara
Ricci, Patrizia
Distrutti, Eleonora
Fiorucci, Stefano
author_facet Biagioli, Michele
Capobianco, Daniela
Carino, Adriana
Marchianò, Silvia
Fiorucci, Chiara
Ricci, Patrizia
Distrutti, Eleonora
Fiorucci, Stefano
author_sort Biagioli, Michele
collection PubMed
description A growing body of evidence suggests that probiotic functionality is not accurately predicted by their taxonomy. Here, we have set up a study to investigate the effectiveness of two probiotic formulations containing a blend of seven bacterial species in modulating intestinal inflammation in two rodent models of colitis, induced by treating mice with 2,4,6-Trinitrobenzenesulfonic acid (TNBS) or dextran sodium sulfate (DSS). Despite the taxonomy of the bacterial species in the two probiotic formulations being similar, only one preparation (Blend 2-Vivomixx) effectively attenuated the development of colitis in both models. In the TNBS model of colitis, Blend 2 reduced the expression of pro-inflammatory genes while increasing the production of anti-inflammatory cytokines, promoting the expansion M2 macrophages and the formation of IL-10-producing Treg cells in the colon’s lamina propria. In the DSS model of colitis, disease attenuation and Treg formation was observed only in mice administered with Blend 2, and this effect was associated with intestinal microbiota remodeling and increased formation of lactate, butyrate, and propionate. None of these effects were observed in mice administered with Blend 1 (VSL#3). In summary, we have shown that two probiotic mixtures obtained by combining taxonomically similar species produced with different manufacturing methods exert divergent effects in mouse models of colitis.
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spelling pubmed-64125852019-03-29 Divergent Effectiveness of Multispecies Probiotic Preparations on Intestinal Microbiota Structure Depends on Metabolic Properties Biagioli, Michele Capobianco, Daniela Carino, Adriana Marchianò, Silvia Fiorucci, Chiara Ricci, Patrizia Distrutti, Eleonora Fiorucci, Stefano Nutrients Article A growing body of evidence suggests that probiotic functionality is not accurately predicted by their taxonomy. Here, we have set up a study to investigate the effectiveness of two probiotic formulations containing a blend of seven bacterial species in modulating intestinal inflammation in two rodent models of colitis, induced by treating mice with 2,4,6-Trinitrobenzenesulfonic acid (TNBS) or dextran sodium sulfate (DSS). Despite the taxonomy of the bacterial species in the two probiotic formulations being similar, only one preparation (Blend 2-Vivomixx) effectively attenuated the development of colitis in both models. In the TNBS model of colitis, Blend 2 reduced the expression of pro-inflammatory genes while increasing the production of anti-inflammatory cytokines, promoting the expansion M2 macrophages and the formation of IL-10-producing Treg cells in the colon’s lamina propria. In the DSS model of colitis, disease attenuation and Treg formation was observed only in mice administered with Blend 2, and this effect was associated with intestinal microbiota remodeling and increased formation of lactate, butyrate, and propionate. None of these effects were observed in mice administered with Blend 1 (VSL#3). In summary, we have shown that two probiotic mixtures obtained by combining taxonomically similar species produced with different manufacturing methods exert divergent effects in mouse models of colitis. MDPI 2019-02-02 /pmc/articles/PMC6412585/ /pubmed/30717413 http://dx.doi.org/10.3390/nu11020325 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Biagioli, Michele
Capobianco, Daniela
Carino, Adriana
Marchianò, Silvia
Fiorucci, Chiara
Ricci, Patrizia
Distrutti, Eleonora
Fiorucci, Stefano
Divergent Effectiveness of Multispecies Probiotic Preparations on Intestinal Microbiota Structure Depends on Metabolic Properties
title Divergent Effectiveness of Multispecies Probiotic Preparations on Intestinal Microbiota Structure Depends on Metabolic Properties
title_full Divergent Effectiveness of Multispecies Probiotic Preparations on Intestinal Microbiota Structure Depends on Metabolic Properties
title_fullStr Divergent Effectiveness of Multispecies Probiotic Preparations on Intestinal Microbiota Structure Depends on Metabolic Properties
title_full_unstemmed Divergent Effectiveness of Multispecies Probiotic Preparations on Intestinal Microbiota Structure Depends on Metabolic Properties
title_short Divergent Effectiveness of Multispecies Probiotic Preparations on Intestinal Microbiota Structure Depends on Metabolic Properties
title_sort divergent effectiveness of multispecies probiotic preparations on intestinal microbiota structure depends on metabolic properties
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412585/
https://www.ncbi.nlm.nih.gov/pubmed/30717413
http://dx.doi.org/10.3390/nu11020325
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