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Transcriptional Dynamics of Grain Development in Barley (Hordeum vulgare L.)

Grain development, as a vital process in the crop’s life cycle, is crucial for determining crop quality and yield. However, the molecular basis and regulatory network of barley grain development is not well understood at present. Here, we investigated the transcriptional dynamics of barley grain dev...

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Autores principales: Bian, Jianxin, Deng, Pingchuan, Zhan, Haoshuang, Wu, Xiaotong, Nishantha, Mutthanthirige D. L. C., Yan, Zhaogui, Du, Xianghong, Nie, Xiaojun, Song, Weining
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412674/
https://www.ncbi.nlm.nih.gov/pubmed/30813307
http://dx.doi.org/10.3390/ijms20040962
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author Bian, Jianxin
Deng, Pingchuan
Zhan, Haoshuang
Wu, Xiaotong
Nishantha, Mutthanthirige D. L. C.
Yan, Zhaogui
Du, Xianghong
Nie, Xiaojun
Song, Weining
author_facet Bian, Jianxin
Deng, Pingchuan
Zhan, Haoshuang
Wu, Xiaotong
Nishantha, Mutthanthirige D. L. C.
Yan, Zhaogui
Du, Xianghong
Nie, Xiaojun
Song, Weining
author_sort Bian, Jianxin
collection PubMed
description Grain development, as a vital process in the crop’s life cycle, is crucial for determining crop quality and yield. However, the molecular basis and regulatory network of barley grain development is not well understood at present. Here, we investigated the transcriptional dynamics of barley grain development through RNA sequencing at four developmental phases, including early prestorage phase (3 days post anthesis (DPA)), late prestorage or transition phase (8 DPA), early storage phase (13 DPA), and levels off stages (18 DPA). Transcriptome profiling found that pronounced shifts occurred in the abundance of transcripts involved in both primary and secondary metabolism during grain development. The transcripts’ activity was decreased during maturation while the largest divergence was observed between the transitions from prestorage phase to storage phase, which coincided with the physiological changes. Furthermore, the transcription factors, hormone signal transduction-related as well as sugar-metabolism-related genes, were found to play a crucial role in barley grain development. Finally, 4771 RNA editing events were identified in these four development stages, and most of the RNA editing genes were preferentially expressed at the prestore stage rather than in the store stage, which was significantly enriched in “essential” genes and plant hormone signal transduction pathway. These results suggested that RNA editing might act as a ‘regulator’ to control grain development. This study systematically dissected the gene expression atlas of barley grain development through transcriptome analysis, which not only provided the potential targets for further functional studies, but also provided insights into the dynamics of gene regulation underlying grain development in barley and beyond.
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spelling pubmed-64126742019-04-05 Transcriptional Dynamics of Grain Development in Barley (Hordeum vulgare L.) Bian, Jianxin Deng, Pingchuan Zhan, Haoshuang Wu, Xiaotong Nishantha, Mutthanthirige D. L. C. Yan, Zhaogui Du, Xianghong Nie, Xiaojun Song, Weining Int J Mol Sci Article Grain development, as a vital process in the crop’s life cycle, is crucial for determining crop quality and yield. However, the molecular basis and regulatory network of barley grain development is not well understood at present. Here, we investigated the transcriptional dynamics of barley grain development through RNA sequencing at four developmental phases, including early prestorage phase (3 days post anthesis (DPA)), late prestorage or transition phase (8 DPA), early storage phase (13 DPA), and levels off stages (18 DPA). Transcriptome profiling found that pronounced shifts occurred in the abundance of transcripts involved in both primary and secondary metabolism during grain development. The transcripts’ activity was decreased during maturation while the largest divergence was observed between the transitions from prestorage phase to storage phase, which coincided with the physiological changes. Furthermore, the transcription factors, hormone signal transduction-related as well as sugar-metabolism-related genes, were found to play a crucial role in barley grain development. Finally, 4771 RNA editing events were identified in these four development stages, and most of the RNA editing genes were preferentially expressed at the prestore stage rather than in the store stage, which was significantly enriched in “essential” genes and plant hormone signal transduction pathway. These results suggested that RNA editing might act as a ‘regulator’ to control grain development. This study systematically dissected the gene expression atlas of barley grain development through transcriptome analysis, which not only provided the potential targets for further functional studies, but also provided insights into the dynamics of gene regulation underlying grain development in barley and beyond. MDPI 2019-02-22 /pmc/articles/PMC6412674/ /pubmed/30813307 http://dx.doi.org/10.3390/ijms20040962 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bian, Jianxin
Deng, Pingchuan
Zhan, Haoshuang
Wu, Xiaotong
Nishantha, Mutthanthirige D. L. C.
Yan, Zhaogui
Du, Xianghong
Nie, Xiaojun
Song, Weining
Transcriptional Dynamics of Grain Development in Barley (Hordeum vulgare L.)
title Transcriptional Dynamics of Grain Development in Barley (Hordeum vulgare L.)
title_full Transcriptional Dynamics of Grain Development in Barley (Hordeum vulgare L.)
title_fullStr Transcriptional Dynamics of Grain Development in Barley (Hordeum vulgare L.)
title_full_unstemmed Transcriptional Dynamics of Grain Development in Barley (Hordeum vulgare L.)
title_short Transcriptional Dynamics of Grain Development in Barley (Hordeum vulgare L.)
title_sort transcriptional dynamics of grain development in barley (hordeum vulgare l.)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412674/
https://www.ncbi.nlm.nih.gov/pubmed/30813307
http://dx.doi.org/10.3390/ijms20040962
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