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Daphnetin: A Novel Anti-Helicobacter pylori Agent

Background: Antibiotic-resistant H. pylori was increasingly found in infected individuals, which resulted in treatment failure and required alternative therapeutic strategies. Daphnetin, a coumarin-derivative compound, has multiple pharmacological activities. Methods: The mechanism of daphnetin on H...

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Autores principales: Wang, Genzhu, Pang, Jing, Hu, Xinxin, Nie, Tongying, Lu, Xi, Li, Xue, Wang, Xiukun, Lu, Yun, Yang, Xinyi, Jiang, Jiandong, Li, Congran, Xiong, Yan Q, You, Xuefu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412720/
https://www.ncbi.nlm.nih.gov/pubmed/30781382
http://dx.doi.org/10.3390/ijms20040850
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author Wang, Genzhu
Pang, Jing
Hu, Xinxin
Nie, Tongying
Lu, Xi
Li, Xue
Wang, Xiukun
Lu, Yun
Yang, Xinyi
Jiang, Jiandong
Li, Congran
Xiong, Yan Q
You, Xuefu
author_facet Wang, Genzhu
Pang, Jing
Hu, Xinxin
Nie, Tongying
Lu, Xi
Li, Xue
Wang, Xiukun
Lu, Yun
Yang, Xinyi
Jiang, Jiandong
Li, Congran
Xiong, Yan Q
You, Xuefu
author_sort Wang, Genzhu
collection PubMed
description Background: Antibiotic-resistant H. pylori was increasingly found in infected individuals, which resulted in treatment failure and required alternative therapeutic strategies. Daphnetin, a coumarin-derivative compound, has multiple pharmacological activities. Methods: The mechanism of daphnetin on H. pylori was investigated focusing on its effect on cell morphologies, transcription of genes related to virulence, adhesion, and cytotoxicity to human gastric epithelial (GES-1) cell line. Results: Daphnetin showed good activities against multidrug resistant (MDR) H. pylori clinical isolates, with minimal inhibitory concentration (MIC) values ranging from 25 to 100 μg/mL. In addition, daphnetin exposure resulted in H. pylori morphological changes. Moreover, daphnetin caused increased translocation of phosphatidylserine (PS), DNA damage, and recA expression, and RecA protein production vs. control group. Of great importance, daphnetin significantly decreased H. pylori adhesion to GES-1 cell line vs. control group, which may be related to the reduced expression of colonization related genes (e.g., babA and ureI). Conclusions: These results suggested that daphnetin has good activity against MDR H. pylori. The mechanism(s) of daphnetin against H. pylori were related to change of membrane structure, increase of DNA damage and PS translocation, and decrease of H. pylori attachment to GES-1 cells.
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spelling pubmed-64127202019-04-05 Daphnetin: A Novel Anti-Helicobacter pylori Agent Wang, Genzhu Pang, Jing Hu, Xinxin Nie, Tongying Lu, Xi Li, Xue Wang, Xiukun Lu, Yun Yang, Xinyi Jiang, Jiandong Li, Congran Xiong, Yan Q You, Xuefu Int J Mol Sci Article Background: Antibiotic-resistant H. pylori was increasingly found in infected individuals, which resulted in treatment failure and required alternative therapeutic strategies. Daphnetin, a coumarin-derivative compound, has multiple pharmacological activities. Methods: The mechanism of daphnetin on H. pylori was investigated focusing on its effect on cell morphologies, transcription of genes related to virulence, adhesion, and cytotoxicity to human gastric epithelial (GES-1) cell line. Results: Daphnetin showed good activities against multidrug resistant (MDR) H. pylori clinical isolates, with minimal inhibitory concentration (MIC) values ranging from 25 to 100 μg/mL. In addition, daphnetin exposure resulted in H. pylori morphological changes. Moreover, daphnetin caused increased translocation of phosphatidylserine (PS), DNA damage, and recA expression, and RecA protein production vs. control group. Of great importance, daphnetin significantly decreased H. pylori adhesion to GES-1 cell line vs. control group, which may be related to the reduced expression of colonization related genes (e.g., babA and ureI). Conclusions: These results suggested that daphnetin has good activity against MDR H. pylori. The mechanism(s) of daphnetin against H. pylori were related to change of membrane structure, increase of DNA damage and PS translocation, and decrease of H. pylori attachment to GES-1 cells. MDPI 2019-02-15 /pmc/articles/PMC6412720/ /pubmed/30781382 http://dx.doi.org/10.3390/ijms20040850 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Genzhu
Pang, Jing
Hu, Xinxin
Nie, Tongying
Lu, Xi
Li, Xue
Wang, Xiukun
Lu, Yun
Yang, Xinyi
Jiang, Jiandong
Li, Congran
Xiong, Yan Q
You, Xuefu
Daphnetin: A Novel Anti-Helicobacter pylori Agent
title Daphnetin: A Novel Anti-Helicobacter pylori Agent
title_full Daphnetin: A Novel Anti-Helicobacter pylori Agent
title_fullStr Daphnetin: A Novel Anti-Helicobacter pylori Agent
title_full_unstemmed Daphnetin: A Novel Anti-Helicobacter pylori Agent
title_short Daphnetin: A Novel Anti-Helicobacter pylori Agent
title_sort daphnetin: a novel anti-helicobacter pylori agent
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412720/
https://www.ncbi.nlm.nih.gov/pubmed/30781382
http://dx.doi.org/10.3390/ijms20040850
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