DNA Repair Gene ZmRAD51A Improves Rice and Arabidopsis Resistance to Disease

RAD51 (DNA repair gene) family genes play ubiquitous roles in immune response among species from plants to mammals. In this study, we cloned the ZmRAD51A gene (a member of RAD51) in maize and generated ZmRAD51A overexpression (ZmRAD51A-OE) in rice, tobacco, and Arabidopsis. The expression level of Z...

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Autores principales: Liu, Fang, Xu, Yunjian, Zhou, Lingyan, Ali, Asif, Jiang, Haiyang, Zhu, Suwen, Li, Xiaoyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412738/
https://www.ncbi.nlm.nih.gov/pubmed/30781829
http://dx.doi.org/10.3390/ijms20040807
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author Liu, Fang
Xu, Yunjian
Zhou, Lingyan
Ali, Asif
Jiang, Haiyang
Zhu, Suwen
Li, Xiaoyu
author_facet Liu, Fang
Xu, Yunjian
Zhou, Lingyan
Ali, Asif
Jiang, Haiyang
Zhu, Suwen
Li, Xiaoyu
author_sort Liu, Fang
collection PubMed
description RAD51 (DNA repair gene) family genes play ubiquitous roles in immune response among species from plants to mammals. In this study, we cloned the ZmRAD51A gene (a member of RAD51) in maize and generated ZmRAD51A overexpression (ZmRAD51A-OE) in rice, tobacco, and Arabidopsis. The expression level of ZmRAD51A was remarkably induced by salicylic acid (SA) application in maize, and the transient overexpression of ZmRAD51A in tobacco induced a hypersensitive response. The disease resistance was significantly enhanced in ZmRAD51A- OE (overexpressing) plants, triggering an increased expression of defense-related genes. High-performance liquid chromatography (HPLC) analysis showed that, compared to control lines, ZmRAD51A-OE in rice plants resulted in higher SA levels, and conferred rice plants resistance to Magnaporthe oryzae. Moreover, the ZmRAD51A-OE Arabidopsis plants displayed increased resistance to Pseudomonas syringae pv. tomato DC3000 when compared to wild types. Together, our results provide the evidence that, for the first time, the maize DNA repair gene ZmRAD51A plays an important role in in disease resistance.
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spelling pubmed-64127382019-04-05 DNA Repair Gene ZmRAD51A Improves Rice and Arabidopsis Resistance to Disease Liu, Fang Xu, Yunjian Zhou, Lingyan Ali, Asif Jiang, Haiyang Zhu, Suwen Li, Xiaoyu Int J Mol Sci Article RAD51 (DNA repair gene) family genes play ubiquitous roles in immune response among species from plants to mammals. In this study, we cloned the ZmRAD51A gene (a member of RAD51) in maize and generated ZmRAD51A overexpression (ZmRAD51A-OE) in rice, tobacco, and Arabidopsis. The expression level of ZmRAD51A was remarkably induced by salicylic acid (SA) application in maize, and the transient overexpression of ZmRAD51A in tobacco induced a hypersensitive response. The disease resistance was significantly enhanced in ZmRAD51A- OE (overexpressing) plants, triggering an increased expression of defense-related genes. High-performance liquid chromatography (HPLC) analysis showed that, compared to control lines, ZmRAD51A-OE in rice plants resulted in higher SA levels, and conferred rice plants resistance to Magnaporthe oryzae. Moreover, the ZmRAD51A-OE Arabidopsis plants displayed increased resistance to Pseudomonas syringae pv. tomato DC3000 when compared to wild types. Together, our results provide the evidence that, for the first time, the maize DNA repair gene ZmRAD51A plays an important role in in disease resistance. MDPI 2019-02-13 /pmc/articles/PMC6412738/ /pubmed/30781829 http://dx.doi.org/10.3390/ijms20040807 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Fang
Xu, Yunjian
Zhou, Lingyan
Ali, Asif
Jiang, Haiyang
Zhu, Suwen
Li, Xiaoyu
DNA Repair Gene ZmRAD51A Improves Rice and Arabidopsis Resistance to Disease
title DNA Repair Gene ZmRAD51A Improves Rice and Arabidopsis Resistance to Disease
title_full DNA Repair Gene ZmRAD51A Improves Rice and Arabidopsis Resistance to Disease
title_fullStr DNA Repair Gene ZmRAD51A Improves Rice and Arabidopsis Resistance to Disease
title_full_unstemmed DNA Repair Gene ZmRAD51A Improves Rice and Arabidopsis Resistance to Disease
title_short DNA Repair Gene ZmRAD51A Improves Rice and Arabidopsis Resistance to Disease
title_sort dna repair gene zmrad51a improves rice and arabidopsis resistance to disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412738/
https://www.ncbi.nlm.nih.gov/pubmed/30781829
http://dx.doi.org/10.3390/ijms20040807
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