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In Situ Image Acquisition and Measurement of Microdroplets Based on Delay Triggering
An in situ image acquisition apparatus based on delay triggering for visualizing microdroplets formation is described. The imaging system includes a charge-coupled device camera, a motion control card, a driving circuit, a time delay triggering circuit, and a light source. By adjusting the varying t...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412821/ https://www.ncbi.nlm.nih.gov/pubmed/30813297 http://dx.doi.org/10.3390/mi10020148 |
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author | Chang, Xuefeng Zheng, Kang Xie, Dan Shu, Xiayun Xu, Keyu Chen, Wenhuan Li, Bo Wu, Changjian |
author_facet | Chang, Xuefeng Zheng, Kang Xie, Dan Shu, Xiayun Xu, Keyu Chen, Wenhuan Li, Bo Wu, Changjian |
author_sort | Chang, Xuefeng |
collection | PubMed |
description | An in situ image acquisition apparatus based on delay triggering for visualizing microdroplets formation is described. The imaging system includes a charge-coupled device camera, a motion control card, a driving circuit, a time delay triggering circuit, and a light source. By adjusting the varying trigger delay time which is synchronized with respect to the signal for jetting, the steady sequential images of the droplet flying in free space can be captured real-time by the system. Several image processing steps are taken to measure the diameters and coordinates of the droplets. Also, the jetting speeds can be calculated according to the delay time interval. For glycerin/water (60:40, mass ratio), under the given conditions of the self-made pneumatically diaphragm-driven drop-on-demand inkjet apparatus, the average of diameter and volume are measured as 266.8 μm and 9944 pL, respectively, and the maximum average velocity of the microdroplets is 0.689 m/s. Finally, the imaging system is applied to measure the volume of 200 microsolder balls generated from the inkjet apparatus. The average diameter is 87.96 μm, and the relative standard deviation is 0.83%. The results show good reproducibility. Unlike previous stroboscopic techniques, the present in situ imaging system which is absence of instantaneous high intensity light employs two control signals to stimulate the microdroplet generator and the charge-coupled device (CCD) camera. Hence, the system can avoid the desynchronization problem of signals which control the strobe light-emitting diode (LED) light source and the camera in previous equipment. This technology is a reliable and cost-effective approach for capturing and measuring microdroplets. |
format | Online Article Text |
id | pubmed-6412821 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64128212019-04-09 In Situ Image Acquisition and Measurement of Microdroplets Based on Delay Triggering Chang, Xuefeng Zheng, Kang Xie, Dan Shu, Xiayun Xu, Keyu Chen, Wenhuan Li, Bo Wu, Changjian Micromachines (Basel) Article An in situ image acquisition apparatus based on delay triggering for visualizing microdroplets formation is described. The imaging system includes a charge-coupled device camera, a motion control card, a driving circuit, a time delay triggering circuit, and a light source. By adjusting the varying trigger delay time which is synchronized with respect to the signal for jetting, the steady sequential images of the droplet flying in free space can be captured real-time by the system. Several image processing steps are taken to measure the diameters and coordinates of the droplets. Also, the jetting speeds can be calculated according to the delay time interval. For glycerin/water (60:40, mass ratio), under the given conditions of the self-made pneumatically diaphragm-driven drop-on-demand inkjet apparatus, the average of diameter and volume are measured as 266.8 μm and 9944 pL, respectively, and the maximum average velocity of the microdroplets is 0.689 m/s. Finally, the imaging system is applied to measure the volume of 200 microsolder balls generated from the inkjet apparatus. The average diameter is 87.96 μm, and the relative standard deviation is 0.83%. The results show good reproducibility. Unlike previous stroboscopic techniques, the present in situ imaging system which is absence of instantaneous high intensity light employs two control signals to stimulate the microdroplet generator and the charge-coupled device (CCD) camera. Hence, the system can avoid the desynchronization problem of signals which control the strobe light-emitting diode (LED) light source and the camera in previous equipment. This technology is a reliable and cost-effective approach for capturing and measuring microdroplets. MDPI 2019-02-22 /pmc/articles/PMC6412821/ /pubmed/30813297 http://dx.doi.org/10.3390/mi10020148 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chang, Xuefeng Zheng, Kang Xie, Dan Shu, Xiayun Xu, Keyu Chen, Wenhuan Li, Bo Wu, Changjian In Situ Image Acquisition and Measurement of Microdroplets Based on Delay Triggering |
title | In Situ Image Acquisition and Measurement of Microdroplets Based on Delay Triggering |
title_full | In Situ Image Acquisition and Measurement of Microdroplets Based on Delay Triggering |
title_fullStr | In Situ Image Acquisition and Measurement of Microdroplets Based on Delay Triggering |
title_full_unstemmed | In Situ Image Acquisition and Measurement of Microdroplets Based on Delay Triggering |
title_short | In Situ Image Acquisition and Measurement of Microdroplets Based on Delay Triggering |
title_sort | in situ image acquisition and measurement of microdroplets based on delay triggering |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412821/ https://www.ncbi.nlm.nih.gov/pubmed/30813297 http://dx.doi.org/10.3390/mi10020148 |
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