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Antimicrobial Potential of Single Metabolites of Curcuma longa Assessed in the Total Extract by Thin-Layer Chromatography-Based Bioautography and Image Analysis

Curcuma longa from Zingiberaceae belongs to the major spices consumed around the world, known from its cholagogue, anti-inflammatory, and antimicrobial properties. Lack of data on the activity of single components of turmeric extract encouraged the authors to apply TLC (thin-layer chromatography) ba...

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Autores principales: Czernicka, Lidia, Grzegorczyk, Agnieszka, Marzec, Zbigniew, Antosiewicz, Beata, Malm, Anna, Kukula-Koch, Wirginia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412823/
https://www.ncbi.nlm.nih.gov/pubmed/30791406
http://dx.doi.org/10.3390/ijms20040898
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author Czernicka, Lidia
Grzegorczyk, Agnieszka
Marzec, Zbigniew
Antosiewicz, Beata
Malm, Anna
Kukula-Koch, Wirginia
author_facet Czernicka, Lidia
Grzegorczyk, Agnieszka
Marzec, Zbigniew
Antosiewicz, Beata
Malm, Anna
Kukula-Koch, Wirginia
author_sort Czernicka, Lidia
collection PubMed
description Curcuma longa from Zingiberaceae belongs to the major spices consumed around the world, known from its cholagogue, anti-inflammatory, and antimicrobial properties. Lack of data on the activity of single components of turmeric extract encouraged the authors to apply TLC (thin-layer chromatography) based bioautography studies to reveal its antimicrobial constituents and construct a universal platform for the bioactivity assessment of crude extracts, with help of a freeware ImageJ software. This optimized chromatographic bioassay performed on diethyl ether and methanol extracts of Curcuma longa was successfully applied on the total extract and revealed the antimicrobial potential of single components against a variety of Gram-positive strains, with no need for their isolation from the mixture. The obtained results were further confronted with a classic microdilution antimicrobial assay on the isolates, purified from the crude extracts by centrifugal partition chromatography in the following solvent system: heptane-chloroform-methanol-water (5:6:3:2) (v/v/v/v).
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spelling pubmed-64128232019-04-05 Antimicrobial Potential of Single Metabolites of Curcuma longa Assessed in the Total Extract by Thin-Layer Chromatography-Based Bioautography and Image Analysis Czernicka, Lidia Grzegorczyk, Agnieszka Marzec, Zbigniew Antosiewicz, Beata Malm, Anna Kukula-Koch, Wirginia Int J Mol Sci Article Curcuma longa from Zingiberaceae belongs to the major spices consumed around the world, known from its cholagogue, anti-inflammatory, and antimicrobial properties. Lack of data on the activity of single components of turmeric extract encouraged the authors to apply TLC (thin-layer chromatography) based bioautography studies to reveal its antimicrobial constituents and construct a universal platform for the bioactivity assessment of crude extracts, with help of a freeware ImageJ software. This optimized chromatographic bioassay performed on diethyl ether and methanol extracts of Curcuma longa was successfully applied on the total extract and revealed the antimicrobial potential of single components against a variety of Gram-positive strains, with no need for their isolation from the mixture. The obtained results were further confronted with a classic microdilution antimicrobial assay on the isolates, purified from the crude extracts by centrifugal partition chromatography in the following solvent system: heptane-chloroform-methanol-water (5:6:3:2) (v/v/v/v). MDPI 2019-02-19 /pmc/articles/PMC6412823/ /pubmed/30791406 http://dx.doi.org/10.3390/ijms20040898 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Czernicka, Lidia
Grzegorczyk, Agnieszka
Marzec, Zbigniew
Antosiewicz, Beata
Malm, Anna
Kukula-Koch, Wirginia
Antimicrobial Potential of Single Metabolites of Curcuma longa Assessed in the Total Extract by Thin-Layer Chromatography-Based Bioautography and Image Analysis
title Antimicrobial Potential of Single Metabolites of Curcuma longa Assessed in the Total Extract by Thin-Layer Chromatography-Based Bioautography and Image Analysis
title_full Antimicrobial Potential of Single Metabolites of Curcuma longa Assessed in the Total Extract by Thin-Layer Chromatography-Based Bioautography and Image Analysis
title_fullStr Antimicrobial Potential of Single Metabolites of Curcuma longa Assessed in the Total Extract by Thin-Layer Chromatography-Based Bioautography and Image Analysis
title_full_unstemmed Antimicrobial Potential of Single Metabolites of Curcuma longa Assessed in the Total Extract by Thin-Layer Chromatography-Based Bioautography and Image Analysis
title_short Antimicrobial Potential of Single Metabolites of Curcuma longa Assessed in the Total Extract by Thin-Layer Chromatography-Based Bioautography and Image Analysis
title_sort antimicrobial potential of single metabolites of curcuma longa assessed in the total extract by thin-layer chromatography-based bioautography and image analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412823/
https://www.ncbi.nlm.nih.gov/pubmed/30791406
http://dx.doi.org/10.3390/ijms20040898
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