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Bunias erucago L.: Glucosinolate Profile and In Vitro Biological Potential

Bunias erucago belongs to the Brassicaceae family, which represents a forgotten crop of the Euro-Mediterranean area. The aim of the present study was to determine the glucosinolate profile in different plant parts and biological properties (antioxidant, anticholinesterase, and cytotoxic activities)...

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Autores principales: Blažević, Ivica, Đulović, Azra, Čikeš Čulić, Vedrana, Burčul, Franko, Ljubenkov, Ivica, Ruščić, Mirko, Generalić Mekinić, Ivana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412829/
https://www.ncbi.nlm.nih.gov/pubmed/30791395
http://dx.doi.org/10.3390/molecules24040741
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author Blažević, Ivica
Đulović, Azra
Čikeš Čulić, Vedrana
Burčul, Franko
Ljubenkov, Ivica
Ruščić, Mirko
Generalić Mekinić, Ivana
author_facet Blažević, Ivica
Đulović, Azra
Čikeš Čulić, Vedrana
Burčul, Franko
Ljubenkov, Ivica
Ruščić, Mirko
Generalić Mekinić, Ivana
author_sort Blažević, Ivica
collection PubMed
description Bunias erucago belongs to the Brassicaceae family, which represents a forgotten crop of the Euro-Mediterranean area. The aim of the present study was to determine the glucosinolate profile in different plant parts and biological properties (antioxidant, anticholinesterase, and cytotoxic activities) of the isolates containing glucosinolate breakdown products. The chemical profiles were determined by using HPLC-PDA-MS/MS of desulfoglucosinolates and GC-MS of glucosinolate degradation products. The analysis of B. erucago showed the presence of seven glucosinolates: gluconapin (1), glucoraphasatin (2), glucoraphenin (3), glucoerucin (4), glucoraphanin (5), glucotropaeolin (6), and glucosinalbin (7). The total glucosinolate content ranged from 7.0 to 14.6 µmol/g of dry weight, with the major glucosinolate glucosinalbin in all parts. The antioxidant activity of all volatile isolates was not notable. At a tested concentration of 227 μg/mL, flower hydro-distillate (FH) showed good AChE inhibition, i.e., 40.9%, while root hydro-distillate (RH) had good activity against BChE, i.e., 54.3%. FH showed the best activity against both tested human bladder cancer cell lines, i.e., against T24 after 72 h, which have IC(50) of 16.0 μg/mL, and against TCCSUP after 48 h with IC(50) of 7.8 μg/mL, and can be considered as highly active. On the other hand, RH showed weak activity against tested cancer cells.
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spelling pubmed-64128292019-04-09 Bunias erucago L.: Glucosinolate Profile and In Vitro Biological Potential Blažević, Ivica Đulović, Azra Čikeš Čulić, Vedrana Burčul, Franko Ljubenkov, Ivica Ruščić, Mirko Generalić Mekinić, Ivana Molecules Article Bunias erucago belongs to the Brassicaceae family, which represents a forgotten crop of the Euro-Mediterranean area. The aim of the present study was to determine the glucosinolate profile in different plant parts and biological properties (antioxidant, anticholinesterase, and cytotoxic activities) of the isolates containing glucosinolate breakdown products. The chemical profiles were determined by using HPLC-PDA-MS/MS of desulfoglucosinolates and GC-MS of glucosinolate degradation products. The analysis of B. erucago showed the presence of seven glucosinolates: gluconapin (1), glucoraphasatin (2), glucoraphenin (3), glucoerucin (4), glucoraphanin (5), glucotropaeolin (6), and glucosinalbin (7). The total glucosinolate content ranged from 7.0 to 14.6 µmol/g of dry weight, with the major glucosinolate glucosinalbin in all parts. The antioxidant activity of all volatile isolates was not notable. At a tested concentration of 227 μg/mL, flower hydro-distillate (FH) showed good AChE inhibition, i.e., 40.9%, while root hydro-distillate (RH) had good activity against BChE, i.e., 54.3%. FH showed the best activity against both tested human bladder cancer cell lines, i.e., against T24 after 72 h, which have IC(50) of 16.0 μg/mL, and against TCCSUP after 48 h with IC(50) of 7.8 μg/mL, and can be considered as highly active. On the other hand, RH showed weak activity against tested cancer cells. MDPI 2019-02-19 /pmc/articles/PMC6412829/ /pubmed/30791395 http://dx.doi.org/10.3390/molecules24040741 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Blažević, Ivica
Đulović, Azra
Čikeš Čulić, Vedrana
Burčul, Franko
Ljubenkov, Ivica
Ruščić, Mirko
Generalić Mekinić, Ivana
Bunias erucago L.: Glucosinolate Profile and In Vitro Biological Potential
title Bunias erucago L.: Glucosinolate Profile and In Vitro Biological Potential
title_full Bunias erucago L.: Glucosinolate Profile and In Vitro Biological Potential
title_fullStr Bunias erucago L.: Glucosinolate Profile and In Vitro Biological Potential
title_full_unstemmed Bunias erucago L.: Glucosinolate Profile and In Vitro Biological Potential
title_short Bunias erucago L.: Glucosinolate Profile and In Vitro Biological Potential
title_sort bunias erucago l.: glucosinolate profile and in vitro biological potential
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412829/
https://www.ncbi.nlm.nih.gov/pubmed/30791395
http://dx.doi.org/10.3390/molecules24040741
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