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Analysis of Metabolite Accumulation Related to Pod Color Variation of Caragana intermedia

Caragana intermedia, a leguminous shrub widely distributed in cold and arid regions, is rich in secondary metabolites and natural active substances, with high nutritional and medical values. It is interesting that the pods of C. intermedia often show different colors among individual plants. In this...

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Autores principales: Yang, Feiyun, Yang, Tianrui, Liu, Kun, Yang, Qi, Wan, Yongqing, Wang, Ruigang, Li, Guojing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412903/
https://www.ncbi.nlm.nih.gov/pubmed/30781495
http://dx.doi.org/10.3390/molecules24040717
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author Yang, Feiyun
Yang, Tianrui
Liu, Kun
Yang, Qi
Wan, Yongqing
Wang, Ruigang
Li, Guojing
author_facet Yang, Feiyun
Yang, Tianrui
Liu, Kun
Yang, Qi
Wan, Yongqing
Wang, Ruigang
Li, Guojing
author_sort Yang, Feiyun
collection PubMed
description Caragana intermedia, a leguminous shrub widely distributed in cold and arid regions, is rich in secondary metabolites and natural active substances, with high nutritional and medical values. It is interesting that the pods of C. intermedia often show different colors among individual plants. In this study, 10-, 20- and 30-day-old red and green pods of C. intermedia were used to identify and characterize important metabolites associated with pod color. A total 557 metabolites, which could be classified into 21 groups, were detected in the pod extracts using liquid chromatography coupled with ESI-triple quadrupole-linear ion trap mass spectrometer (LC-ESI-MS/MS). Metabolomics analysis revealed significant differences in 15 groups of metabolites between red and green pods, including amino acids, nucleotide derivatives, flavonoids, and phytohormones. Metabolic pathway analysis showed that the shikimic acid and the phytohormone metabolic pathways were extraordinarily active in red pods, and the difference between red and green pods was obvious. Moreover, red pods showed remarkable flavonoids, cytokinins, and auxin accumulation, and the content of total flavonoids and proanthocyanidins in 30-day-old red pods was significantly higher than that in green pods. This metabolic profile contributes to valuable insights into the metabolic regulation mechanism in different color pods.
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spelling pubmed-64129032019-04-09 Analysis of Metabolite Accumulation Related to Pod Color Variation of Caragana intermedia Yang, Feiyun Yang, Tianrui Liu, Kun Yang, Qi Wan, Yongqing Wang, Ruigang Li, Guojing Molecules Article Caragana intermedia, a leguminous shrub widely distributed in cold and arid regions, is rich in secondary metabolites and natural active substances, with high nutritional and medical values. It is interesting that the pods of C. intermedia often show different colors among individual plants. In this study, 10-, 20- and 30-day-old red and green pods of C. intermedia were used to identify and characterize important metabolites associated with pod color. A total 557 metabolites, which could be classified into 21 groups, were detected in the pod extracts using liquid chromatography coupled with ESI-triple quadrupole-linear ion trap mass spectrometer (LC-ESI-MS/MS). Metabolomics analysis revealed significant differences in 15 groups of metabolites between red and green pods, including amino acids, nucleotide derivatives, flavonoids, and phytohormones. Metabolic pathway analysis showed that the shikimic acid and the phytohormone metabolic pathways were extraordinarily active in red pods, and the difference between red and green pods was obvious. Moreover, red pods showed remarkable flavonoids, cytokinins, and auxin accumulation, and the content of total flavonoids and proanthocyanidins in 30-day-old red pods was significantly higher than that in green pods. This metabolic profile contributes to valuable insights into the metabolic regulation mechanism in different color pods. MDPI 2019-02-16 /pmc/articles/PMC6412903/ /pubmed/30781495 http://dx.doi.org/10.3390/molecules24040717 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Feiyun
Yang, Tianrui
Liu, Kun
Yang, Qi
Wan, Yongqing
Wang, Ruigang
Li, Guojing
Analysis of Metabolite Accumulation Related to Pod Color Variation of Caragana intermedia
title Analysis of Metabolite Accumulation Related to Pod Color Variation of Caragana intermedia
title_full Analysis of Metabolite Accumulation Related to Pod Color Variation of Caragana intermedia
title_fullStr Analysis of Metabolite Accumulation Related to Pod Color Variation of Caragana intermedia
title_full_unstemmed Analysis of Metabolite Accumulation Related to Pod Color Variation of Caragana intermedia
title_short Analysis of Metabolite Accumulation Related to Pod Color Variation of Caragana intermedia
title_sort analysis of metabolite accumulation related to pod color variation of caragana intermedia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412903/
https://www.ncbi.nlm.nih.gov/pubmed/30781495
http://dx.doi.org/10.3390/molecules24040717
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