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Analysis of Metabolite Accumulation Related to Pod Color Variation of Caragana intermedia
Caragana intermedia, a leguminous shrub widely distributed in cold and arid regions, is rich in secondary metabolites and natural active substances, with high nutritional and medical values. It is interesting that the pods of C. intermedia often show different colors among individual plants. In this...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412903/ https://www.ncbi.nlm.nih.gov/pubmed/30781495 http://dx.doi.org/10.3390/molecules24040717 |
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author | Yang, Feiyun Yang, Tianrui Liu, Kun Yang, Qi Wan, Yongqing Wang, Ruigang Li, Guojing |
author_facet | Yang, Feiyun Yang, Tianrui Liu, Kun Yang, Qi Wan, Yongqing Wang, Ruigang Li, Guojing |
author_sort | Yang, Feiyun |
collection | PubMed |
description | Caragana intermedia, a leguminous shrub widely distributed in cold and arid regions, is rich in secondary metabolites and natural active substances, with high nutritional and medical values. It is interesting that the pods of C. intermedia often show different colors among individual plants. In this study, 10-, 20- and 30-day-old red and green pods of C. intermedia were used to identify and characterize important metabolites associated with pod color. A total 557 metabolites, which could be classified into 21 groups, were detected in the pod extracts using liquid chromatography coupled with ESI-triple quadrupole-linear ion trap mass spectrometer (LC-ESI-MS/MS). Metabolomics analysis revealed significant differences in 15 groups of metabolites between red and green pods, including amino acids, nucleotide derivatives, flavonoids, and phytohormones. Metabolic pathway analysis showed that the shikimic acid and the phytohormone metabolic pathways were extraordinarily active in red pods, and the difference between red and green pods was obvious. Moreover, red pods showed remarkable flavonoids, cytokinins, and auxin accumulation, and the content of total flavonoids and proanthocyanidins in 30-day-old red pods was significantly higher than that in green pods. This metabolic profile contributes to valuable insights into the metabolic regulation mechanism in different color pods. |
format | Online Article Text |
id | pubmed-6412903 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64129032019-04-09 Analysis of Metabolite Accumulation Related to Pod Color Variation of Caragana intermedia Yang, Feiyun Yang, Tianrui Liu, Kun Yang, Qi Wan, Yongqing Wang, Ruigang Li, Guojing Molecules Article Caragana intermedia, a leguminous shrub widely distributed in cold and arid regions, is rich in secondary metabolites and natural active substances, with high nutritional and medical values. It is interesting that the pods of C. intermedia often show different colors among individual plants. In this study, 10-, 20- and 30-day-old red and green pods of C. intermedia were used to identify and characterize important metabolites associated with pod color. A total 557 metabolites, which could be classified into 21 groups, were detected in the pod extracts using liquid chromatography coupled with ESI-triple quadrupole-linear ion trap mass spectrometer (LC-ESI-MS/MS). Metabolomics analysis revealed significant differences in 15 groups of metabolites between red and green pods, including amino acids, nucleotide derivatives, flavonoids, and phytohormones. Metabolic pathway analysis showed that the shikimic acid and the phytohormone metabolic pathways were extraordinarily active in red pods, and the difference between red and green pods was obvious. Moreover, red pods showed remarkable flavonoids, cytokinins, and auxin accumulation, and the content of total flavonoids and proanthocyanidins in 30-day-old red pods was significantly higher than that in green pods. This metabolic profile contributes to valuable insights into the metabolic regulation mechanism in different color pods. MDPI 2019-02-16 /pmc/articles/PMC6412903/ /pubmed/30781495 http://dx.doi.org/10.3390/molecules24040717 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yang, Feiyun Yang, Tianrui Liu, Kun Yang, Qi Wan, Yongqing Wang, Ruigang Li, Guojing Analysis of Metabolite Accumulation Related to Pod Color Variation of Caragana intermedia |
title | Analysis of Metabolite Accumulation Related to Pod Color Variation of Caragana intermedia |
title_full | Analysis of Metabolite Accumulation Related to Pod Color Variation of Caragana intermedia |
title_fullStr | Analysis of Metabolite Accumulation Related to Pod Color Variation of Caragana intermedia |
title_full_unstemmed | Analysis of Metabolite Accumulation Related to Pod Color Variation of Caragana intermedia |
title_short | Analysis of Metabolite Accumulation Related to Pod Color Variation of Caragana intermedia |
title_sort | analysis of metabolite accumulation related to pod color variation of caragana intermedia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412903/ https://www.ncbi.nlm.nih.gov/pubmed/30781495 http://dx.doi.org/10.3390/molecules24040717 |
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