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Analysis of Extracellular Vesicles in Gastric Juice from Gastric Cancer Patients

Extracellular vesicles (EVs) are secretory membrane vesicles containing lipids, proteins, and nucleic acids; they function in intercellular transport by delivering their components to recipient cells. EVs are observed in various body fluids, i.e., blood, saliva, urine, amniotic fluid, and ascites. E...

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Autores principales: Kagota, Shuji, Taniguchi, Kohei, Lee, Sang-Woong, Ito, Yuko, Kuranaga, Yuki, Hashiguchi, Yasuyuki, Inomata, Yosuke, Imai, Yoshiro, Tanaka, Ryo, Tashiro, Keitaro, Kawai, Masaru, Akao, Yukihiro, Uchiyama, Kazuhisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412909/
https://www.ncbi.nlm.nih.gov/pubmed/30813244
http://dx.doi.org/10.3390/ijms20040953
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author Kagota, Shuji
Taniguchi, Kohei
Lee, Sang-Woong
Ito, Yuko
Kuranaga, Yuki
Hashiguchi, Yasuyuki
Inomata, Yosuke
Imai, Yoshiro
Tanaka, Ryo
Tashiro, Keitaro
Kawai, Masaru
Akao, Yukihiro
Uchiyama, Kazuhisa
author_facet Kagota, Shuji
Taniguchi, Kohei
Lee, Sang-Woong
Ito, Yuko
Kuranaga, Yuki
Hashiguchi, Yasuyuki
Inomata, Yosuke
Imai, Yoshiro
Tanaka, Ryo
Tashiro, Keitaro
Kawai, Masaru
Akao, Yukihiro
Uchiyama, Kazuhisa
author_sort Kagota, Shuji
collection PubMed
description Extracellular vesicles (EVs) are secretory membrane vesicles containing lipids, proteins, and nucleic acids; they function in intercellular transport by delivering their components to recipient cells. EVs are observed in various body fluids, i.e., blood, saliva, urine, amniotic fluid, and ascites. EVs secreted from cancer cells play important roles in the formation of their environment, including fibrosis, angiogenesis, evasion of immune surveillance, and even metastasis. However, EVs in gastric juice (GJ-EVs) have been largely unexplored. In this study, we sought to clarify the existence of GJ-EVs derived from gastric cancer patients. GJ-EVs were isolated by the ultracentrifuge method combined with our own preprocessing from gastric cancer (GC) patients. We verified GJ-EVs by morphological experiments, i.e., nanoparticle tracking system analysis and electron microscopy. In addition, protein and microRNA markers of EVs were examined by Western blotting analysis, Bioanalyzer, or quantitative reverse transcription polymerase chain reaction. GJ-EVs were found to promote the proliferation of normal fibroblast cells. Our findings suggest that isolates from the GJ of GC patients contain EVs and imply that GJ-EVs partially affect their microenvironments and that analysis using GJ-EVs from GC patients will help to clarify the pathophysiology of GC.
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spelling pubmed-64129092019-03-29 Analysis of Extracellular Vesicles in Gastric Juice from Gastric Cancer Patients Kagota, Shuji Taniguchi, Kohei Lee, Sang-Woong Ito, Yuko Kuranaga, Yuki Hashiguchi, Yasuyuki Inomata, Yosuke Imai, Yoshiro Tanaka, Ryo Tashiro, Keitaro Kawai, Masaru Akao, Yukihiro Uchiyama, Kazuhisa Int J Mol Sci Article Extracellular vesicles (EVs) are secretory membrane vesicles containing lipids, proteins, and nucleic acids; they function in intercellular transport by delivering their components to recipient cells. EVs are observed in various body fluids, i.e., blood, saliva, urine, amniotic fluid, and ascites. EVs secreted from cancer cells play important roles in the formation of their environment, including fibrosis, angiogenesis, evasion of immune surveillance, and even metastasis. However, EVs in gastric juice (GJ-EVs) have been largely unexplored. In this study, we sought to clarify the existence of GJ-EVs derived from gastric cancer patients. GJ-EVs were isolated by the ultracentrifuge method combined with our own preprocessing from gastric cancer (GC) patients. We verified GJ-EVs by morphological experiments, i.e., nanoparticle tracking system analysis and electron microscopy. In addition, protein and microRNA markers of EVs were examined by Western blotting analysis, Bioanalyzer, or quantitative reverse transcription polymerase chain reaction. GJ-EVs were found to promote the proliferation of normal fibroblast cells. Our findings suggest that isolates from the GJ of GC patients contain EVs and imply that GJ-EVs partially affect their microenvironments and that analysis using GJ-EVs from GC patients will help to clarify the pathophysiology of GC. MDPI 2019-02-22 /pmc/articles/PMC6412909/ /pubmed/30813244 http://dx.doi.org/10.3390/ijms20040953 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kagota, Shuji
Taniguchi, Kohei
Lee, Sang-Woong
Ito, Yuko
Kuranaga, Yuki
Hashiguchi, Yasuyuki
Inomata, Yosuke
Imai, Yoshiro
Tanaka, Ryo
Tashiro, Keitaro
Kawai, Masaru
Akao, Yukihiro
Uchiyama, Kazuhisa
Analysis of Extracellular Vesicles in Gastric Juice from Gastric Cancer Patients
title Analysis of Extracellular Vesicles in Gastric Juice from Gastric Cancer Patients
title_full Analysis of Extracellular Vesicles in Gastric Juice from Gastric Cancer Patients
title_fullStr Analysis of Extracellular Vesicles in Gastric Juice from Gastric Cancer Patients
title_full_unstemmed Analysis of Extracellular Vesicles in Gastric Juice from Gastric Cancer Patients
title_short Analysis of Extracellular Vesicles in Gastric Juice from Gastric Cancer Patients
title_sort analysis of extracellular vesicles in gastric juice from gastric cancer patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412909/
https://www.ncbi.nlm.nih.gov/pubmed/30813244
http://dx.doi.org/10.3390/ijms20040953
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