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Analysis of Extracellular Vesicles in Gastric Juice from Gastric Cancer Patients
Extracellular vesicles (EVs) are secretory membrane vesicles containing lipids, proteins, and nucleic acids; they function in intercellular transport by delivering their components to recipient cells. EVs are observed in various body fluids, i.e., blood, saliva, urine, amniotic fluid, and ascites. E...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412909/ https://www.ncbi.nlm.nih.gov/pubmed/30813244 http://dx.doi.org/10.3390/ijms20040953 |
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author | Kagota, Shuji Taniguchi, Kohei Lee, Sang-Woong Ito, Yuko Kuranaga, Yuki Hashiguchi, Yasuyuki Inomata, Yosuke Imai, Yoshiro Tanaka, Ryo Tashiro, Keitaro Kawai, Masaru Akao, Yukihiro Uchiyama, Kazuhisa |
author_facet | Kagota, Shuji Taniguchi, Kohei Lee, Sang-Woong Ito, Yuko Kuranaga, Yuki Hashiguchi, Yasuyuki Inomata, Yosuke Imai, Yoshiro Tanaka, Ryo Tashiro, Keitaro Kawai, Masaru Akao, Yukihiro Uchiyama, Kazuhisa |
author_sort | Kagota, Shuji |
collection | PubMed |
description | Extracellular vesicles (EVs) are secretory membrane vesicles containing lipids, proteins, and nucleic acids; they function in intercellular transport by delivering their components to recipient cells. EVs are observed in various body fluids, i.e., blood, saliva, urine, amniotic fluid, and ascites. EVs secreted from cancer cells play important roles in the formation of their environment, including fibrosis, angiogenesis, evasion of immune surveillance, and even metastasis. However, EVs in gastric juice (GJ-EVs) have been largely unexplored. In this study, we sought to clarify the existence of GJ-EVs derived from gastric cancer patients. GJ-EVs were isolated by the ultracentrifuge method combined with our own preprocessing from gastric cancer (GC) patients. We verified GJ-EVs by morphological experiments, i.e., nanoparticle tracking system analysis and electron microscopy. In addition, protein and microRNA markers of EVs were examined by Western blotting analysis, Bioanalyzer, or quantitative reverse transcription polymerase chain reaction. GJ-EVs were found to promote the proliferation of normal fibroblast cells. Our findings suggest that isolates from the GJ of GC patients contain EVs and imply that GJ-EVs partially affect their microenvironments and that analysis using GJ-EVs from GC patients will help to clarify the pathophysiology of GC. |
format | Online Article Text |
id | pubmed-6412909 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64129092019-03-29 Analysis of Extracellular Vesicles in Gastric Juice from Gastric Cancer Patients Kagota, Shuji Taniguchi, Kohei Lee, Sang-Woong Ito, Yuko Kuranaga, Yuki Hashiguchi, Yasuyuki Inomata, Yosuke Imai, Yoshiro Tanaka, Ryo Tashiro, Keitaro Kawai, Masaru Akao, Yukihiro Uchiyama, Kazuhisa Int J Mol Sci Article Extracellular vesicles (EVs) are secretory membrane vesicles containing lipids, proteins, and nucleic acids; they function in intercellular transport by delivering their components to recipient cells. EVs are observed in various body fluids, i.e., blood, saliva, urine, amniotic fluid, and ascites. EVs secreted from cancer cells play important roles in the formation of their environment, including fibrosis, angiogenesis, evasion of immune surveillance, and even metastasis. However, EVs in gastric juice (GJ-EVs) have been largely unexplored. In this study, we sought to clarify the existence of GJ-EVs derived from gastric cancer patients. GJ-EVs were isolated by the ultracentrifuge method combined with our own preprocessing from gastric cancer (GC) patients. We verified GJ-EVs by morphological experiments, i.e., nanoparticle tracking system analysis and electron microscopy. In addition, protein and microRNA markers of EVs were examined by Western blotting analysis, Bioanalyzer, or quantitative reverse transcription polymerase chain reaction. GJ-EVs were found to promote the proliferation of normal fibroblast cells. Our findings suggest that isolates from the GJ of GC patients contain EVs and imply that GJ-EVs partially affect their microenvironments and that analysis using GJ-EVs from GC patients will help to clarify the pathophysiology of GC. MDPI 2019-02-22 /pmc/articles/PMC6412909/ /pubmed/30813244 http://dx.doi.org/10.3390/ijms20040953 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kagota, Shuji Taniguchi, Kohei Lee, Sang-Woong Ito, Yuko Kuranaga, Yuki Hashiguchi, Yasuyuki Inomata, Yosuke Imai, Yoshiro Tanaka, Ryo Tashiro, Keitaro Kawai, Masaru Akao, Yukihiro Uchiyama, Kazuhisa Analysis of Extracellular Vesicles in Gastric Juice from Gastric Cancer Patients |
title | Analysis of Extracellular Vesicles in Gastric Juice from Gastric Cancer Patients |
title_full | Analysis of Extracellular Vesicles in Gastric Juice from Gastric Cancer Patients |
title_fullStr | Analysis of Extracellular Vesicles in Gastric Juice from Gastric Cancer Patients |
title_full_unstemmed | Analysis of Extracellular Vesicles in Gastric Juice from Gastric Cancer Patients |
title_short | Analysis of Extracellular Vesicles in Gastric Juice from Gastric Cancer Patients |
title_sort | analysis of extracellular vesicles in gastric juice from gastric cancer patients |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412909/ https://www.ncbi.nlm.nih.gov/pubmed/30813244 http://dx.doi.org/10.3390/ijms20040953 |
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