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MicroRNA-766-3p Contributes to Anti-Inflammatory Responses through the Indirect Inhibition of NF-κB Signaling

MicroRNA (miRNA) is small RNA of 20 to 22 nucleotides in length and is stably present in plasma. Regulating the expression of miRNA taken into cells has been suggested as a general therapeutic approach. We identified the novel anti-inflammatory miRNA hsa-miR-766-3p and investigated its biological fu...

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Autores principales: Hayakawa, Kunihiro, Kawasaki, Mikiko, Hirai, Takuya, Yoshida, Yuko, Tsushima, Hiroshi, Fujishiro, Maki, Ikeda, Keigo, Morimoto, Shinji, Takamori, Kenji, Sekigawa, Iwao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6413049/
https://www.ncbi.nlm.nih.gov/pubmed/30769772
http://dx.doi.org/10.3390/ijms20040809
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author Hayakawa, Kunihiro
Kawasaki, Mikiko
Hirai, Takuya
Yoshida, Yuko
Tsushima, Hiroshi
Fujishiro, Maki
Ikeda, Keigo
Morimoto, Shinji
Takamori, Kenji
Sekigawa, Iwao
author_facet Hayakawa, Kunihiro
Kawasaki, Mikiko
Hirai, Takuya
Yoshida, Yuko
Tsushima, Hiroshi
Fujishiro, Maki
Ikeda, Keigo
Morimoto, Shinji
Takamori, Kenji
Sekigawa, Iwao
author_sort Hayakawa, Kunihiro
collection PubMed
description MicroRNA (miRNA) is small RNA of 20 to 22 nucleotides in length and is stably present in plasma. Regulating the expression of miRNA taken into cells has been suggested as a general therapeutic approach. We identified the novel anti-inflammatory miRNA hsa-miR-766-3p and investigated its biological function in human rheumatoid arthritis (RA) fibroblast-like synoviocyte MH7A cells. To verify the function of the miRNA present in the plasma of RA patients, we performed a comprehensive analysis of the miRNA expression during abatacept treatment and identified eight miRNAs with significantly altered expression levels. Among these eight miRNAs, miR-766-3p was found to have a clear function. The expression of inflammatory genes in response to inflammatory stimuli was suppressed in MH7A transduced with miR-766-3p. We showed that miR-766-3p indirectly reduced the activation of NF-κB and clarified that this mechanism was partially involved in the reduction of the mineralocorticoid receptor expression. In addition, the inflammatory responses were suppressed in other types of cells. These results indicate the novel function of miR-766-3p, findings that may aid in the development of therapies to suppress inflammation, not only in RA but also in other diseases.
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spelling pubmed-64130492019-03-29 MicroRNA-766-3p Contributes to Anti-Inflammatory Responses through the Indirect Inhibition of NF-κB Signaling Hayakawa, Kunihiro Kawasaki, Mikiko Hirai, Takuya Yoshida, Yuko Tsushima, Hiroshi Fujishiro, Maki Ikeda, Keigo Morimoto, Shinji Takamori, Kenji Sekigawa, Iwao Int J Mol Sci Article MicroRNA (miRNA) is small RNA of 20 to 22 nucleotides in length and is stably present in plasma. Regulating the expression of miRNA taken into cells has been suggested as a general therapeutic approach. We identified the novel anti-inflammatory miRNA hsa-miR-766-3p and investigated its biological function in human rheumatoid arthritis (RA) fibroblast-like synoviocyte MH7A cells. To verify the function of the miRNA present in the plasma of RA patients, we performed a comprehensive analysis of the miRNA expression during abatacept treatment and identified eight miRNAs with significantly altered expression levels. Among these eight miRNAs, miR-766-3p was found to have a clear function. The expression of inflammatory genes in response to inflammatory stimuli was suppressed in MH7A transduced with miR-766-3p. We showed that miR-766-3p indirectly reduced the activation of NF-κB and clarified that this mechanism was partially involved in the reduction of the mineralocorticoid receptor expression. In addition, the inflammatory responses were suppressed in other types of cells. These results indicate the novel function of miR-766-3p, findings that may aid in the development of therapies to suppress inflammation, not only in RA but also in other diseases. MDPI 2019-02-14 /pmc/articles/PMC6413049/ /pubmed/30769772 http://dx.doi.org/10.3390/ijms20040809 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hayakawa, Kunihiro
Kawasaki, Mikiko
Hirai, Takuya
Yoshida, Yuko
Tsushima, Hiroshi
Fujishiro, Maki
Ikeda, Keigo
Morimoto, Shinji
Takamori, Kenji
Sekigawa, Iwao
MicroRNA-766-3p Contributes to Anti-Inflammatory Responses through the Indirect Inhibition of NF-κB Signaling
title MicroRNA-766-3p Contributes to Anti-Inflammatory Responses through the Indirect Inhibition of NF-κB Signaling
title_full MicroRNA-766-3p Contributes to Anti-Inflammatory Responses through the Indirect Inhibition of NF-κB Signaling
title_fullStr MicroRNA-766-3p Contributes to Anti-Inflammatory Responses through the Indirect Inhibition of NF-κB Signaling
title_full_unstemmed MicroRNA-766-3p Contributes to Anti-Inflammatory Responses through the Indirect Inhibition of NF-κB Signaling
title_short MicroRNA-766-3p Contributes to Anti-Inflammatory Responses through the Indirect Inhibition of NF-κB Signaling
title_sort microrna-766-3p contributes to anti-inflammatory responses through the indirect inhibition of nf-κb signaling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6413049/
https://www.ncbi.nlm.nih.gov/pubmed/30769772
http://dx.doi.org/10.3390/ijms20040809
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