Cargando…

Activin B Stimulates Mouse Vibrissae Growth and Regulates Cell Proliferation and Cell Cycle Progression of Hair Matrix Cells through ERK Signaling

Activins and their receptors play important roles in the control of hair follicle morphogenesis, but their role in vibrissae follicle growth remains unclear. To investigate the effect of Activin B on vibrissae follicles, the anagen induction assay and an in vitro vibrissae culture system were constr...

Descripción completa

Detalles Bibliográficos
Autores principales: Tang, Pei, Wang, Xueer, Zhang, Min, Huang, Simin, Lin, Chuxi, Yan, Fang, Deng, Ying, Zhang, Lu, Zhang, Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6413065/
https://www.ncbi.nlm.nih.gov/pubmed/30781441
http://dx.doi.org/10.3390/ijms20040853
_version_ 1783402752123076608
author Tang, Pei
Wang, Xueer
Zhang, Min
Huang, Simin
Lin, Chuxi
Yan, Fang
Deng, Ying
Zhang, Lu
Zhang, Lin
author_facet Tang, Pei
Wang, Xueer
Zhang, Min
Huang, Simin
Lin, Chuxi
Yan, Fang
Deng, Ying
Zhang, Lu
Zhang, Lin
author_sort Tang, Pei
collection PubMed
description Activins and their receptors play important roles in the control of hair follicle morphogenesis, but their role in vibrissae follicle growth remains unclear. To investigate the effect of Activin B on vibrissae follicles, the anagen induction assay and an in vitro vibrissae culture system were constructed. Hematoxylin and eosin staining were performed to determine the hair cycle stages. The 5-ethynyl-2′-deoxyuridine (EdU) and Cell Counting Kit-8 (CCK-8) assays were used to examine the cell proliferation. Flow cytometry was used to detect the cell cycle phase. Inhibitors and Western blot analysis were used to investigate the signaling pathway induced by Activin B. As a result, we found that the vibrissae follicle growth was accelerated by 10 ng/mL Activin B in the anagen induction assay and in an organ culture model. 10 ng/mL Activin B promoted hair matrix cell proliferation in vivo and in vitro. Moreover, Activin B modulates hair matrix cell growth through the ERK–Elk1 signaling pathway, and Activin B accelerates hair matrix cell transition from the G1/G0 phase to the S phase through the ERK–Cyclin D1 signaling pathway. Taken together, these results demonstrated that Activin B may promote mouse vibrissae growth by stimulating hair matrix cell proliferation and cell cycle progression through ERK signaling.
format Online
Article
Text
id pubmed-6413065
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-64130652019-03-29 Activin B Stimulates Mouse Vibrissae Growth and Regulates Cell Proliferation and Cell Cycle Progression of Hair Matrix Cells through ERK Signaling Tang, Pei Wang, Xueer Zhang, Min Huang, Simin Lin, Chuxi Yan, Fang Deng, Ying Zhang, Lu Zhang, Lin Int J Mol Sci Article Activins and their receptors play important roles in the control of hair follicle morphogenesis, but their role in vibrissae follicle growth remains unclear. To investigate the effect of Activin B on vibrissae follicles, the anagen induction assay and an in vitro vibrissae culture system were constructed. Hematoxylin and eosin staining were performed to determine the hair cycle stages. The 5-ethynyl-2′-deoxyuridine (EdU) and Cell Counting Kit-8 (CCK-8) assays were used to examine the cell proliferation. Flow cytometry was used to detect the cell cycle phase. Inhibitors and Western blot analysis were used to investigate the signaling pathway induced by Activin B. As a result, we found that the vibrissae follicle growth was accelerated by 10 ng/mL Activin B in the anagen induction assay and in an organ culture model. 10 ng/mL Activin B promoted hair matrix cell proliferation in vivo and in vitro. Moreover, Activin B modulates hair matrix cell growth through the ERK–Elk1 signaling pathway, and Activin B accelerates hair matrix cell transition from the G1/G0 phase to the S phase through the ERK–Cyclin D1 signaling pathway. Taken together, these results demonstrated that Activin B may promote mouse vibrissae growth by stimulating hair matrix cell proliferation and cell cycle progression through ERK signaling. MDPI 2019-02-15 /pmc/articles/PMC6413065/ /pubmed/30781441 http://dx.doi.org/10.3390/ijms20040853 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tang, Pei
Wang, Xueer
Zhang, Min
Huang, Simin
Lin, Chuxi
Yan, Fang
Deng, Ying
Zhang, Lu
Zhang, Lin
Activin B Stimulates Mouse Vibrissae Growth and Regulates Cell Proliferation and Cell Cycle Progression of Hair Matrix Cells through ERK Signaling
title Activin B Stimulates Mouse Vibrissae Growth and Regulates Cell Proliferation and Cell Cycle Progression of Hair Matrix Cells through ERK Signaling
title_full Activin B Stimulates Mouse Vibrissae Growth and Regulates Cell Proliferation and Cell Cycle Progression of Hair Matrix Cells through ERK Signaling
title_fullStr Activin B Stimulates Mouse Vibrissae Growth and Regulates Cell Proliferation and Cell Cycle Progression of Hair Matrix Cells through ERK Signaling
title_full_unstemmed Activin B Stimulates Mouse Vibrissae Growth and Regulates Cell Proliferation and Cell Cycle Progression of Hair Matrix Cells through ERK Signaling
title_short Activin B Stimulates Mouse Vibrissae Growth and Regulates Cell Proliferation and Cell Cycle Progression of Hair Matrix Cells through ERK Signaling
title_sort activin b stimulates mouse vibrissae growth and regulates cell proliferation and cell cycle progression of hair matrix cells through erk signaling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6413065/
https://www.ncbi.nlm.nih.gov/pubmed/30781441
http://dx.doi.org/10.3390/ijms20040853
work_keys_str_mv AT tangpei activinbstimulatesmousevibrissaegrowthandregulatescellproliferationandcellcycleprogressionofhairmatrixcellsthrougherksignaling
AT wangxueer activinbstimulatesmousevibrissaegrowthandregulatescellproliferationandcellcycleprogressionofhairmatrixcellsthrougherksignaling
AT zhangmin activinbstimulatesmousevibrissaegrowthandregulatescellproliferationandcellcycleprogressionofhairmatrixcellsthrougherksignaling
AT huangsimin activinbstimulatesmousevibrissaegrowthandregulatescellproliferationandcellcycleprogressionofhairmatrixcellsthrougherksignaling
AT linchuxi activinbstimulatesmousevibrissaegrowthandregulatescellproliferationandcellcycleprogressionofhairmatrixcellsthrougherksignaling
AT yanfang activinbstimulatesmousevibrissaegrowthandregulatescellproliferationandcellcycleprogressionofhairmatrixcellsthrougherksignaling
AT dengying activinbstimulatesmousevibrissaegrowthandregulatescellproliferationandcellcycleprogressionofhairmatrixcellsthrougherksignaling
AT zhanglu activinbstimulatesmousevibrissaegrowthandregulatescellproliferationandcellcycleprogressionofhairmatrixcellsthrougherksignaling
AT zhanglin activinbstimulatesmousevibrissaegrowthandregulatescellproliferationandcellcycleprogressionofhairmatrixcellsthrougherksignaling