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Role of PD-L1 Expression in Non-Small Cell Lung Cancer and Their Prognostic Significance according to Clinicopathological Factors and Diagnostic Markers
Background: The latest immunotherapy, used in the treatment of non-small cell lung cancer (NSCLC), uses monoclonal antibodies directed against programmed death ligand 1 (PD-L1) to inhibit its interaction with the PD-1 receptor. Elevated levels of PD-L1 expression were observed on NSCLC cells. The as...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6413136/ https://www.ncbi.nlm.nih.gov/pubmed/30769852 http://dx.doi.org/10.3390/ijms20040824 |
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author | Pawelczyk, Konrad Piotrowska, Aleksandra Ciesielska, Urszula Jablonska, Karolina Glatzel-Plucinska, Natalia Grzegrzolka, Jedrzej Podhorska-Okolow, Marzenna Dziegiel, Piotr Nowinska, Katarzyna |
author_facet | Pawelczyk, Konrad Piotrowska, Aleksandra Ciesielska, Urszula Jablonska, Karolina Glatzel-Plucinska, Natalia Grzegrzolka, Jedrzej Podhorska-Okolow, Marzenna Dziegiel, Piotr Nowinska, Katarzyna |
author_sort | Pawelczyk, Konrad |
collection | PubMed |
description | Background: The latest immunotherapy, used in the treatment of non-small cell lung cancer (NSCLC), uses monoclonal antibodies directed against programmed death ligand 1 (PD-L1) to inhibit its interaction with the PD-1 receptor. Elevated levels of PD-L1 expression were observed on NSCLC cells. The association between PD-L1 expression and clinicopathological features is still unclear. Therefore, we examined this relationship and also compare PD-L1 expression levels with Ki-67, p63 and TTF-1. Methods: 866 samples of NSCLCs were used to prepare tissue microarrays (TMAs) on which immunohistochemical (IHC) reactions were performed. Changes in the level of CD274 (PD-L1) gene expression in 62 NSCLC tumors were tested in relation to 14 normal lung tissues by real-time PCR reactions (RT-PCR). Results: PD-L1 expression was observed in 32.6% of NSCLCs. PD-L1 expression was increased in higher malignancy grades (G) (p < 0.0001) and in higher lymph node status (pN) (p = 0.0428). The patients with low PD-L1 expression had longer overall survival compared to the group with high expression (p = 0.0332) in adenocarcinoma (AC) only. Conclusions: PD-L1 expression seems to be associated with increased tumor proliferation and aggressiveness as well as shorter patient survival in NSCLC, predominantly in the AC group. |
format | Online Article Text |
id | pubmed-6413136 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64131362019-03-29 Role of PD-L1 Expression in Non-Small Cell Lung Cancer and Their Prognostic Significance according to Clinicopathological Factors and Diagnostic Markers Pawelczyk, Konrad Piotrowska, Aleksandra Ciesielska, Urszula Jablonska, Karolina Glatzel-Plucinska, Natalia Grzegrzolka, Jedrzej Podhorska-Okolow, Marzenna Dziegiel, Piotr Nowinska, Katarzyna Int J Mol Sci Article Background: The latest immunotherapy, used in the treatment of non-small cell lung cancer (NSCLC), uses monoclonal antibodies directed against programmed death ligand 1 (PD-L1) to inhibit its interaction with the PD-1 receptor. Elevated levels of PD-L1 expression were observed on NSCLC cells. The association between PD-L1 expression and clinicopathological features is still unclear. Therefore, we examined this relationship and also compare PD-L1 expression levels with Ki-67, p63 and TTF-1. Methods: 866 samples of NSCLCs were used to prepare tissue microarrays (TMAs) on which immunohistochemical (IHC) reactions were performed. Changes in the level of CD274 (PD-L1) gene expression in 62 NSCLC tumors were tested in relation to 14 normal lung tissues by real-time PCR reactions (RT-PCR). Results: PD-L1 expression was observed in 32.6% of NSCLCs. PD-L1 expression was increased in higher malignancy grades (G) (p < 0.0001) and in higher lymph node status (pN) (p = 0.0428). The patients with low PD-L1 expression had longer overall survival compared to the group with high expression (p = 0.0332) in adenocarcinoma (AC) only. Conclusions: PD-L1 expression seems to be associated with increased tumor proliferation and aggressiveness as well as shorter patient survival in NSCLC, predominantly in the AC group. MDPI 2019-02-14 /pmc/articles/PMC6413136/ /pubmed/30769852 http://dx.doi.org/10.3390/ijms20040824 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pawelczyk, Konrad Piotrowska, Aleksandra Ciesielska, Urszula Jablonska, Karolina Glatzel-Plucinska, Natalia Grzegrzolka, Jedrzej Podhorska-Okolow, Marzenna Dziegiel, Piotr Nowinska, Katarzyna Role of PD-L1 Expression in Non-Small Cell Lung Cancer and Their Prognostic Significance according to Clinicopathological Factors and Diagnostic Markers |
title | Role of PD-L1 Expression in Non-Small Cell Lung Cancer and Their Prognostic Significance according to Clinicopathological Factors and Diagnostic Markers |
title_full | Role of PD-L1 Expression in Non-Small Cell Lung Cancer and Their Prognostic Significance according to Clinicopathological Factors and Diagnostic Markers |
title_fullStr | Role of PD-L1 Expression in Non-Small Cell Lung Cancer and Their Prognostic Significance according to Clinicopathological Factors and Diagnostic Markers |
title_full_unstemmed | Role of PD-L1 Expression in Non-Small Cell Lung Cancer and Their Prognostic Significance according to Clinicopathological Factors and Diagnostic Markers |
title_short | Role of PD-L1 Expression in Non-Small Cell Lung Cancer and Their Prognostic Significance according to Clinicopathological Factors and Diagnostic Markers |
title_sort | role of pd-l1 expression in non-small cell lung cancer and their prognostic significance according to clinicopathological factors and diagnostic markers |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6413136/ https://www.ncbi.nlm.nih.gov/pubmed/30769852 http://dx.doi.org/10.3390/ijms20040824 |
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