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Role of PD-L1 Expression in Non-Small Cell Lung Cancer and Their Prognostic Significance according to Clinicopathological Factors and Diagnostic Markers

Background: The latest immunotherapy, used in the treatment of non-small cell lung cancer (NSCLC), uses monoclonal antibodies directed against programmed death ligand 1 (PD-L1) to inhibit its interaction with the PD-1 receptor. Elevated levels of PD-L1 expression were observed on NSCLC cells. The as...

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Autores principales: Pawelczyk, Konrad, Piotrowska, Aleksandra, Ciesielska, Urszula, Jablonska, Karolina, Glatzel-Plucinska, Natalia, Grzegrzolka, Jedrzej, Podhorska-Okolow, Marzenna, Dziegiel, Piotr, Nowinska, Katarzyna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6413136/
https://www.ncbi.nlm.nih.gov/pubmed/30769852
http://dx.doi.org/10.3390/ijms20040824
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author Pawelczyk, Konrad
Piotrowska, Aleksandra
Ciesielska, Urszula
Jablonska, Karolina
Glatzel-Plucinska, Natalia
Grzegrzolka, Jedrzej
Podhorska-Okolow, Marzenna
Dziegiel, Piotr
Nowinska, Katarzyna
author_facet Pawelczyk, Konrad
Piotrowska, Aleksandra
Ciesielska, Urszula
Jablonska, Karolina
Glatzel-Plucinska, Natalia
Grzegrzolka, Jedrzej
Podhorska-Okolow, Marzenna
Dziegiel, Piotr
Nowinska, Katarzyna
author_sort Pawelczyk, Konrad
collection PubMed
description Background: The latest immunotherapy, used in the treatment of non-small cell lung cancer (NSCLC), uses monoclonal antibodies directed against programmed death ligand 1 (PD-L1) to inhibit its interaction with the PD-1 receptor. Elevated levels of PD-L1 expression were observed on NSCLC cells. The association between PD-L1 expression and clinicopathological features is still unclear. Therefore, we examined this relationship and also compare PD-L1 expression levels with Ki-67, p63 and TTF-1. Methods: 866 samples of NSCLCs were used to prepare tissue microarrays (TMAs) on which immunohistochemical (IHC) reactions were performed. Changes in the level of CD274 (PD-L1) gene expression in 62 NSCLC tumors were tested in relation to 14 normal lung tissues by real-time PCR reactions (RT-PCR). Results: PD-L1 expression was observed in 32.6% of NSCLCs. PD-L1 expression was increased in higher malignancy grades (G) (p < 0.0001) and in higher lymph node status (pN) (p = 0.0428). The patients with low PD-L1 expression had longer overall survival compared to the group with high expression (p = 0.0332) in adenocarcinoma (AC) only. Conclusions: PD-L1 expression seems to be associated with increased tumor proliferation and aggressiveness as well as shorter patient survival in NSCLC, predominantly in the AC group.
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spelling pubmed-64131362019-03-29 Role of PD-L1 Expression in Non-Small Cell Lung Cancer and Their Prognostic Significance according to Clinicopathological Factors and Diagnostic Markers Pawelczyk, Konrad Piotrowska, Aleksandra Ciesielska, Urszula Jablonska, Karolina Glatzel-Plucinska, Natalia Grzegrzolka, Jedrzej Podhorska-Okolow, Marzenna Dziegiel, Piotr Nowinska, Katarzyna Int J Mol Sci Article Background: The latest immunotherapy, used in the treatment of non-small cell lung cancer (NSCLC), uses monoclonal antibodies directed against programmed death ligand 1 (PD-L1) to inhibit its interaction with the PD-1 receptor. Elevated levels of PD-L1 expression were observed on NSCLC cells. The association between PD-L1 expression and clinicopathological features is still unclear. Therefore, we examined this relationship and also compare PD-L1 expression levels with Ki-67, p63 and TTF-1. Methods: 866 samples of NSCLCs were used to prepare tissue microarrays (TMAs) on which immunohistochemical (IHC) reactions were performed. Changes in the level of CD274 (PD-L1) gene expression in 62 NSCLC tumors were tested in relation to 14 normal lung tissues by real-time PCR reactions (RT-PCR). Results: PD-L1 expression was observed in 32.6% of NSCLCs. PD-L1 expression was increased in higher malignancy grades (G) (p < 0.0001) and in higher lymph node status (pN) (p = 0.0428). The patients with low PD-L1 expression had longer overall survival compared to the group with high expression (p = 0.0332) in adenocarcinoma (AC) only. Conclusions: PD-L1 expression seems to be associated with increased tumor proliferation and aggressiveness as well as shorter patient survival in NSCLC, predominantly in the AC group. MDPI 2019-02-14 /pmc/articles/PMC6413136/ /pubmed/30769852 http://dx.doi.org/10.3390/ijms20040824 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pawelczyk, Konrad
Piotrowska, Aleksandra
Ciesielska, Urszula
Jablonska, Karolina
Glatzel-Plucinska, Natalia
Grzegrzolka, Jedrzej
Podhorska-Okolow, Marzenna
Dziegiel, Piotr
Nowinska, Katarzyna
Role of PD-L1 Expression in Non-Small Cell Lung Cancer and Their Prognostic Significance according to Clinicopathological Factors and Diagnostic Markers
title Role of PD-L1 Expression in Non-Small Cell Lung Cancer and Their Prognostic Significance according to Clinicopathological Factors and Diagnostic Markers
title_full Role of PD-L1 Expression in Non-Small Cell Lung Cancer and Their Prognostic Significance according to Clinicopathological Factors and Diagnostic Markers
title_fullStr Role of PD-L1 Expression in Non-Small Cell Lung Cancer and Their Prognostic Significance according to Clinicopathological Factors and Diagnostic Markers
title_full_unstemmed Role of PD-L1 Expression in Non-Small Cell Lung Cancer and Their Prognostic Significance according to Clinicopathological Factors and Diagnostic Markers
title_short Role of PD-L1 Expression in Non-Small Cell Lung Cancer and Their Prognostic Significance according to Clinicopathological Factors and Diagnostic Markers
title_sort role of pd-l1 expression in non-small cell lung cancer and their prognostic significance according to clinicopathological factors and diagnostic markers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6413136/
https://www.ncbi.nlm.nih.gov/pubmed/30769852
http://dx.doi.org/10.3390/ijms20040824
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