A Chemiluminescent Method for the Detection of H(2)O(2) and Glucose Based on Intrinsic Peroxidase-Like Activity of WS(2) Quantum Dots

Currently, researchers are looking for nanomaterials with peroxidase-like activity to replace natural peroxidase enzymes. For this purpose, WS(2) quantum dots (WS(2) QDs) were synthesized via a solvothermal method, which improved the mimetic behavior. The resulting WS(2) QDs with a size of 1–1.5 nm had a high fluorescence emission, dependent on the excitation wavelength. WS(2) QDs with uniform morphology showed a high catalytic effect in destroying H(2)O(2). The peroxidase-like activity of synthesized nanostructures was studied in H(2)O(2) chemical and electrochemical reduction systems. The mimetic effect of WS(2) QDs was also shown in an H(2)O(2)–rhodamine B (RB) chemiluminescence system. For this aim, a stopped-flow chemiluminescence (CL) detection system was applied. Also, in order to confirm the peroxidase-like effect of quantum dots, colorimetry and electrochemical techniques were used. In the enzymatic reaction of glucose, H(2)O(2) is one of the products which can be determined. Under optimum conditions, H(2)O(2) can be detected in the concentration range of 0–1000 nmol·L(−1), with a detection limit of 2.4 nmol·L(−1). Using this CL assay, a linear relationship was obtained between the intensity of the CL emission and glucose concentration in the range of 0.01–30 nmol·L(−1), with a limit of detection (3S) of 4.2 nmol·L(−1).