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Early Peritoneal CC Chemokine Production Correlates with Divergent Inflammatory Phenotypes and Susceptibility to Experimental Arthritis in Mice

The inflammatory and autoimmune events preceding clinical symptoms in rheumatoid arthritis (RA) and other autoimmune diseases are difficult to study in human patients. Therefore, animal models that share immunologic and clinical features with human RA, such as pristane-induced arthritis (PIA), are v...

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Autores principales: Rossato, Cristiano, Albuquerque, Layra Lucy, Katz, Iana Suly Santos, Borrego, Andrea, Cabrera, Wafa Hanna Koury, Spadafora-Ferreira, Mônica, Ribeiro, Orlando Garcia, Starobinas, Nancy, Ibañez, Olga Martinez, De Franco, Marcelo, Jensen, José Ricardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6413398/
https://www.ncbi.nlm.nih.gov/pubmed/30937315
http://dx.doi.org/10.1155/2019/2641098
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author Rossato, Cristiano
Albuquerque, Layra Lucy
Katz, Iana Suly Santos
Borrego, Andrea
Cabrera, Wafa Hanna Koury
Spadafora-Ferreira, Mônica
Ribeiro, Orlando Garcia
Starobinas, Nancy
Ibañez, Olga Martinez
De Franco, Marcelo
Jensen, José Ricardo
author_facet Rossato, Cristiano
Albuquerque, Layra Lucy
Katz, Iana Suly Santos
Borrego, Andrea
Cabrera, Wafa Hanna Koury
Spadafora-Ferreira, Mônica
Ribeiro, Orlando Garcia
Starobinas, Nancy
Ibañez, Olga Martinez
De Franco, Marcelo
Jensen, José Ricardo
author_sort Rossato, Cristiano
collection PubMed
description The inflammatory and autoimmune events preceding clinical symptoms in rheumatoid arthritis (RA) and other autoimmune diseases are difficult to study in human patients. Therefore, animal models that share immunologic and clinical features with human RA, such as pristane-induced arthritis (PIA), are valuable tools for assessing the primordial events related to arthritis susceptibility. PIA-resistant HIII and susceptible LIII mice were injected i.p. with pristane, and peritoneal lavage fluid was harvested in the early (7 days) and late (35 days) preclinical phases of PIA. Chemokine and cytokine levels were measured in lavage supernatant with ELISA, peritoneal inflammatory leukocytes were immunophenotyped by flow cytometry, and gene expression was determined by qRT-PCR. Leukocyte recruitment was quantitatively and qualitatively divergent in the peritoneum of HIII and LIII mice, with an early increase of CC chemokines (CCL2/CCL3/CCL5/CCL12/CCL22) in the susceptible LIII strain. Also, cytokines such as IL-12p40, IL-23, and IL-18 were elevated in LIII mice while IL-6 was increased in HIII animals. The results show that an early peritoneal CC chemokine response is an important feature of arthritis susceptibility and defines potential biomarkers in this model.
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spelling pubmed-64133982019-04-01 Early Peritoneal CC Chemokine Production Correlates with Divergent Inflammatory Phenotypes and Susceptibility to Experimental Arthritis in Mice Rossato, Cristiano Albuquerque, Layra Lucy Katz, Iana Suly Santos Borrego, Andrea Cabrera, Wafa Hanna Koury Spadafora-Ferreira, Mônica Ribeiro, Orlando Garcia Starobinas, Nancy Ibañez, Olga Martinez De Franco, Marcelo Jensen, José Ricardo J Immunol Res Research Article The inflammatory and autoimmune events preceding clinical symptoms in rheumatoid arthritis (RA) and other autoimmune diseases are difficult to study in human patients. Therefore, animal models that share immunologic and clinical features with human RA, such as pristane-induced arthritis (PIA), are valuable tools for assessing the primordial events related to arthritis susceptibility. PIA-resistant HIII and susceptible LIII mice were injected i.p. with pristane, and peritoneal lavage fluid was harvested in the early (7 days) and late (35 days) preclinical phases of PIA. Chemokine and cytokine levels were measured in lavage supernatant with ELISA, peritoneal inflammatory leukocytes were immunophenotyped by flow cytometry, and gene expression was determined by qRT-PCR. Leukocyte recruitment was quantitatively and qualitatively divergent in the peritoneum of HIII and LIII mice, with an early increase of CC chemokines (CCL2/CCL3/CCL5/CCL12/CCL22) in the susceptible LIII strain. Also, cytokines such as IL-12p40, IL-23, and IL-18 were elevated in LIII mice while IL-6 was increased in HIII animals. The results show that an early peritoneal CC chemokine response is an important feature of arthritis susceptibility and defines potential biomarkers in this model. Hindawi 2019-02-26 /pmc/articles/PMC6413398/ /pubmed/30937315 http://dx.doi.org/10.1155/2019/2641098 Text en Copyright © 2019 Cristiano Rossato et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Rossato, Cristiano
Albuquerque, Layra Lucy
Katz, Iana Suly Santos
Borrego, Andrea
Cabrera, Wafa Hanna Koury
Spadafora-Ferreira, Mônica
Ribeiro, Orlando Garcia
Starobinas, Nancy
Ibañez, Olga Martinez
De Franco, Marcelo
Jensen, José Ricardo
Early Peritoneal CC Chemokine Production Correlates with Divergent Inflammatory Phenotypes and Susceptibility to Experimental Arthritis in Mice
title Early Peritoneal CC Chemokine Production Correlates with Divergent Inflammatory Phenotypes and Susceptibility to Experimental Arthritis in Mice
title_full Early Peritoneal CC Chemokine Production Correlates with Divergent Inflammatory Phenotypes and Susceptibility to Experimental Arthritis in Mice
title_fullStr Early Peritoneal CC Chemokine Production Correlates with Divergent Inflammatory Phenotypes and Susceptibility to Experimental Arthritis in Mice
title_full_unstemmed Early Peritoneal CC Chemokine Production Correlates with Divergent Inflammatory Phenotypes and Susceptibility to Experimental Arthritis in Mice
title_short Early Peritoneal CC Chemokine Production Correlates with Divergent Inflammatory Phenotypes and Susceptibility to Experimental Arthritis in Mice
title_sort early peritoneal cc chemokine production correlates with divergent inflammatory phenotypes and susceptibility to experimental arthritis in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6413398/
https://www.ncbi.nlm.nih.gov/pubmed/30937315
http://dx.doi.org/10.1155/2019/2641098
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