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A multicellular way of life for a multipartite virus
A founding paradigm in virology is that the spatial unit of the viral replication cycle is an individual cell. Multipartite viruses have a segmented genome where each segment is encapsidated separately. In this situation the viral genome is not recapitulated in a single virus particle but in the vir...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6414197/ https://www.ncbi.nlm.nih.gov/pubmed/30857590 http://dx.doi.org/10.7554/eLife.43599 |
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author | Sicard, Anne Pirolles, Elodie Gallet, Romain Vernerey, Marie-Stéphanie Yvon, Michel Urbino, Cica Peterschmitt, Michel Gutierrez, Serafin Michalakis, Yannis Blanc, Stéphane |
author_facet | Sicard, Anne Pirolles, Elodie Gallet, Romain Vernerey, Marie-Stéphanie Yvon, Michel Urbino, Cica Peterschmitt, Michel Gutierrez, Serafin Michalakis, Yannis Blanc, Stéphane |
author_sort | Sicard, Anne |
collection | PubMed |
description | A founding paradigm in virology is that the spatial unit of the viral replication cycle is an individual cell. Multipartite viruses have a segmented genome where each segment is encapsidated separately. In this situation the viral genome is not recapitulated in a single virus particle but in the viral population. How multipartite viruses manage to efficiently infect individual cells with all segments, thus with the whole genome information, is a long-standing but perhaps deceptive mystery. By localizing and quantifying the genome segments of a nanovirus in host plant tissues we show that they rarely co-occur within individual cells. We further demonstrate that distinct segments accumulate independently in different cells and that the viral system is functional through complementation across cells. Our observation deviates from the classical conceptual framework in virology and opens an alternative possibility (at least for nanoviruses) where the infection can operate at a level above the individual cell level, defining a viral multicellular way of life. |
format | Online Article Text |
id | pubmed-6414197 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-64141972019-03-14 A multicellular way of life for a multipartite virus Sicard, Anne Pirolles, Elodie Gallet, Romain Vernerey, Marie-Stéphanie Yvon, Michel Urbino, Cica Peterschmitt, Michel Gutierrez, Serafin Michalakis, Yannis Blanc, Stéphane eLife Microbiology and Infectious Disease A founding paradigm in virology is that the spatial unit of the viral replication cycle is an individual cell. Multipartite viruses have a segmented genome where each segment is encapsidated separately. In this situation the viral genome is not recapitulated in a single virus particle but in the viral population. How multipartite viruses manage to efficiently infect individual cells with all segments, thus with the whole genome information, is a long-standing but perhaps deceptive mystery. By localizing and quantifying the genome segments of a nanovirus in host plant tissues we show that they rarely co-occur within individual cells. We further demonstrate that distinct segments accumulate independently in different cells and that the viral system is functional through complementation across cells. Our observation deviates from the classical conceptual framework in virology and opens an alternative possibility (at least for nanoviruses) where the infection can operate at a level above the individual cell level, defining a viral multicellular way of life. eLife Sciences Publications, Ltd 2019-03-12 /pmc/articles/PMC6414197/ /pubmed/30857590 http://dx.doi.org/10.7554/eLife.43599 Text en © 2019, Sicard et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Microbiology and Infectious Disease Sicard, Anne Pirolles, Elodie Gallet, Romain Vernerey, Marie-Stéphanie Yvon, Michel Urbino, Cica Peterschmitt, Michel Gutierrez, Serafin Michalakis, Yannis Blanc, Stéphane A multicellular way of life for a multipartite virus |
title | A multicellular way of life for a multipartite virus |
title_full | A multicellular way of life for a multipartite virus |
title_fullStr | A multicellular way of life for a multipartite virus |
title_full_unstemmed | A multicellular way of life for a multipartite virus |
title_short | A multicellular way of life for a multipartite virus |
title_sort | multicellular way of life for a multipartite virus |
topic | Microbiology and Infectious Disease |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6414197/ https://www.ncbi.nlm.nih.gov/pubmed/30857590 http://dx.doi.org/10.7554/eLife.43599 |
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