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Antibacterial Films Made of Ionic Complexes of Poly(γ-glutamic acid) and Ethyl Lauroyl Arginate

The biocide agent LAE (ethyl (α)N-lauroyl l-arginate chloride) was coupled with poly(γ-glutamic acid) (PGGA) to form stable ionic complexes with LAE:PGGA ratios of 1 and 0.5. The nanostructure adopted by these complexes and its response to thermal changes were examined in detail by Differential scan...

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Autores principales: Gamarra-Montes, Ana, Missagia, Beatriz, Morató, Jordi, Muñoz-Guerra, Sebastián
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6414889/
https://www.ncbi.nlm.nih.gov/pubmed/30966055
http://dx.doi.org/10.3390/polym10010021
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author Gamarra-Montes, Ana
Missagia, Beatriz
Morató, Jordi
Muñoz-Guerra, Sebastián
author_facet Gamarra-Montes, Ana
Missagia, Beatriz
Morató, Jordi
Muñoz-Guerra, Sebastián
author_sort Gamarra-Montes, Ana
collection PubMed
description The biocide agent LAE (ethyl (α)N-lauroyl l-arginate chloride) was coupled with poly(γ-glutamic acid) (PGGA) to form stable ionic complexes with LAE:PGGA ratios of 1 and 0.5. The nanostructure adopted by these complexes and its response to thermal changes were examined in detail by Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) using synchrotron radiation in real time. A layered biphasic structure with LAE filling the space between the polypeptidic sheets was adopted in these complexes. The complexes were stable up to above 250 °C, non-water soluble, and were able to form consistent transparent films. The release of LAE from the complexes upon incubation in aqueous buffer was examined and found to depend on both pH and complex composition. The antibacterial activity of films made of these complexes against Gram-positive (L. monocytogenes and S. aureus) and Gram-negative (E. coli and S. enterica) bacteria was preliminary evaluated and was found to be very high against the formers and only moderate against the later. The bactericide activity displayed by the LAE·PGGA complexes was directly related with the amount of LAE that was released from the film to the environment.
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spelling pubmed-64148892019-04-02 Antibacterial Films Made of Ionic Complexes of Poly(γ-glutamic acid) and Ethyl Lauroyl Arginate Gamarra-Montes, Ana Missagia, Beatriz Morató, Jordi Muñoz-Guerra, Sebastián Polymers (Basel) Article The biocide agent LAE (ethyl (α)N-lauroyl l-arginate chloride) was coupled with poly(γ-glutamic acid) (PGGA) to form stable ionic complexes with LAE:PGGA ratios of 1 and 0.5. The nanostructure adopted by these complexes and its response to thermal changes were examined in detail by Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) using synchrotron radiation in real time. A layered biphasic structure with LAE filling the space between the polypeptidic sheets was adopted in these complexes. The complexes were stable up to above 250 °C, non-water soluble, and were able to form consistent transparent films. The release of LAE from the complexes upon incubation in aqueous buffer was examined and found to depend on both pH and complex composition. The antibacterial activity of films made of these complexes against Gram-positive (L. monocytogenes and S. aureus) and Gram-negative (E. coli and S. enterica) bacteria was preliminary evaluated and was found to be very high against the formers and only moderate against the later. The bactericide activity displayed by the LAE·PGGA complexes was directly related with the amount of LAE that was released from the film to the environment. MDPI 2017-12-24 /pmc/articles/PMC6414889/ /pubmed/30966055 http://dx.doi.org/10.3390/polym10010021 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gamarra-Montes, Ana
Missagia, Beatriz
Morató, Jordi
Muñoz-Guerra, Sebastián
Antibacterial Films Made of Ionic Complexes of Poly(γ-glutamic acid) and Ethyl Lauroyl Arginate
title Antibacterial Films Made of Ionic Complexes of Poly(γ-glutamic acid) and Ethyl Lauroyl Arginate
title_full Antibacterial Films Made of Ionic Complexes of Poly(γ-glutamic acid) and Ethyl Lauroyl Arginate
title_fullStr Antibacterial Films Made of Ionic Complexes of Poly(γ-glutamic acid) and Ethyl Lauroyl Arginate
title_full_unstemmed Antibacterial Films Made of Ionic Complexes of Poly(γ-glutamic acid) and Ethyl Lauroyl Arginate
title_short Antibacterial Films Made of Ionic Complexes of Poly(γ-glutamic acid) and Ethyl Lauroyl Arginate
title_sort antibacterial films made of ionic complexes of poly(γ-glutamic acid) and ethyl lauroyl arginate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6414889/
https://www.ncbi.nlm.nih.gov/pubmed/30966055
http://dx.doi.org/10.3390/polym10010021
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