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Surface Hydrophilicity of Poly(l-Lactide) Acid Polymer Film Changes the Human Adult Adipose Stem Cell Architecture

Current knowledge indicates that the molecular cross-talk between stem cells and biomaterials guides the stem cells’ fate within a tissue engineering system. In this work, we have explored the effects of the interaction between the poly(l-lactide) acid (PLLA) polymer film and human adult adipose ste...

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Autores principales: Argentati, Chiara, Morena, Francesco, Montanucci, Pia, Rallini, Marco, Basta, Giuseppe, Calabrese, Nicolino, Calafiore, Riccardo, Cordellini, Marino, Emiliani, Carla, Armentano, Ilaria, Martino, Sabata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6414915/
https://www.ncbi.nlm.nih.gov/pubmed/30966176
http://dx.doi.org/10.3390/polym10020140
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author Argentati, Chiara
Morena, Francesco
Montanucci, Pia
Rallini, Marco
Basta, Giuseppe
Calabrese, Nicolino
Calafiore, Riccardo
Cordellini, Marino
Emiliani, Carla
Armentano, Ilaria
Martino, Sabata
author_facet Argentati, Chiara
Morena, Francesco
Montanucci, Pia
Rallini, Marco
Basta, Giuseppe
Calabrese, Nicolino
Calafiore, Riccardo
Cordellini, Marino
Emiliani, Carla
Armentano, Ilaria
Martino, Sabata
author_sort Argentati, Chiara
collection PubMed
description Current knowledge indicates that the molecular cross-talk between stem cells and biomaterials guides the stem cells’ fate within a tissue engineering system. In this work, we have explored the effects of the interaction between the poly(l-lactide) acid (PLLA) polymer film and human adult adipose stem cells (hASCs), focusing on the events correlating the materials’ surface characteristics and the cells’ plasma membrane. hASCs were seeded on films of pristine PLLA polymer and on a PLLA surface modified by the radiofrequency plasma method under oxygen flow (PLLA+O(2)). Comparative experiments were performed using human bone-marrow mesenchymal stem cells (hBM-MSCs) and human umbilical matrix stem cells (hUCMSCs). After treatment with oxygen-plasma, the surface of PLLA films became hydrophilic, whereas the bulk properties were not affected. hASCs cultured on pristine PLLA polymer films acquired a spheroid conformation. On the contrary, hASCs seeded on PLLA+O(2) film surface maintained the fibroblast-like morphology typically observed on tissue culture polystyrene. This suggests that the surface hydrophilicity is involved in the acquisition of the spheroid conformation. Noteworthy, the oxygen treatment had no effects on hBM-MSC and hUCMSC cultures and both stem cells maintained the same shape observed on PLLA films. This different behavior suggests that the biomaterial-interaction is stem cell specific.
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spelling pubmed-64149152019-04-02 Surface Hydrophilicity of Poly(l-Lactide) Acid Polymer Film Changes the Human Adult Adipose Stem Cell Architecture Argentati, Chiara Morena, Francesco Montanucci, Pia Rallini, Marco Basta, Giuseppe Calabrese, Nicolino Calafiore, Riccardo Cordellini, Marino Emiliani, Carla Armentano, Ilaria Martino, Sabata Polymers (Basel) Article Current knowledge indicates that the molecular cross-talk between stem cells and biomaterials guides the stem cells’ fate within a tissue engineering system. In this work, we have explored the effects of the interaction between the poly(l-lactide) acid (PLLA) polymer film and human adult adipose stem cells (hASCs), focusing on the events correlating the materials’ surface characteristics and the cells’ plasma membrane. hASCs were seeded on films of pristine PLLA polymer and on a PLLA surface modified by the radiofrequency plasma method under oxygen flow (PLLA+O(2)). Comparative experiments were performed using human bone-marrow mesenchymal stem cells (hBM-MSCs) and human umbilical matrix stem cells (hUCMSCs). After treatment with oxygen-plasma, the surface of PLLA films became hydrophilic, whereas the bulk properties were not affected. hASCs cultured on pristine PLLA polymer films acquired a spheroid conformation. On the contrary, hASCs seeded on PLLA+O(2) film surface maintained the fibroblast-like morphology typically observed on tissue culture polystyrene. This suggests that the surface hydrophilicity is involved in the acquisition of the spheroid conformation. Noteworthy, the oxygen treatment had no effects on hBM-MSC and hUCMSC cultures and both stem cells maintained the same shape observed on PLLA films. This different behavior suggests that the biomaterial-interaction is stem cell specific. MDPI 2018-02-01 /pmc/articles/PMC6414915/ /pubmed/30966176 http://dx.doi.org/10.3390/polym10020140 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Argentati, Chiara
Morena, Francesco
Montanucci, Pia
Rallini, Marco
Basta, Giuseppe
Calabrese, Nicolino
Calafiore, Riccardo
Cordellini, Marino
Emiliani, Carla
Armentano, Ilaria
Martino, Sabata
Surface Hydrophilicity of Poly(l-Lactide) Acid Polymer Film Changes the Human Adult Adipose Stem Cell Architecture
title Surface Hydrophilicity of Poly(l-Lactide) Acid Polymer Film Changes the Human Adult Adipose Stem Cell Architecture
title_full Surface Hydrophilicity of Poly(l-Lactide) Acid Polymer Film Changes the Human Adult Adipose Stem Cell Architecture
title_fullStr Surface Hydrophilicity of Poly(l-Lactide) Acid Polymer Film Changes the Human Adult Adipose Stem Cell Architecture
title_full_unstemmed Surface Hydrophilicity of Poly(l-Lactide) Acid Polymer Film Changes the Human Adult Adipose Stem Cell Architecture
title_short Surface Hydrophilicity of Poly(l-Lactide) Acid Polymer Film Changes the Human Adult Adipose Stem Cell Architecture
title_sort surface hydrophilicity of poly(l-lactide) acid polymer film changes the human adult adipose stem cell architecture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6414915/
https://www.ncbi.nlm.nih.gov/pubmed/30966176
http://dx.doi.org/10.3390/polym10020140
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