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Facile and Rapid Formation of Giant Vesicles from Glass Beads

Giant vesicles (GVs) are widely-used model systems for biological membranes. The formulation of these vesicles, however, can be problematic and artifacts, such as degraded molecules or left-over oil, may be present in the final liposomes. The rapid formulation of a high number of artifact-free vesic...

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Detalles Bibliográficos
Autores principales: Tanasescu, Radu, Mettal, Ute, Colom, Adai, Roux, Aurélien, Zumbuehl, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6414939/
https://www.ncbi.nlm.nih.gov/pubmed/30966090
http://dx.doi.org/10.3390/polym10010054
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author Tanasescu, Radu
Mettal, Ute
Colom, Adai
Roux, Aurélien
Zumbuehl, Andreas
author_facet Tanasescu, Radu
Mettal, Ute
Colom, Adai
Roux, Aurélien
Zumbuehl, Andreas
author_sort Tanasescu, Radu
collection PubMed
description Giant vesicles (GVs) are widely-used model systems for biological membranes. The formulation of these vesicles, however, can be problematic and artifacts, such as degraded molecules or left-over oil, may be present in the final liposomes. The rapid formulation of a high number of artifact-free vesicles of uniform size using standard laboratory equipment is, therefore, highly desirable. Here, the gentle hydration method of glass bead-supported thin lipid films has been enhanced by adding a vortexing step. This led to the formulation of a uniform population of giant vesicles. Batches of glass beads coated with different lipids can be combined to produce vesicles of hybrid lipid compositions. This method represents a stable approach to rapidly generate giant vesicles.
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spelling pubmed-64149392019-04-02 Facile and Rapid Formation of Giant Vesicles from Glass Beads Tanasescu, Radu Mettal, Ute Colom, Adai Roux, Aurélien Zumbuehl, Andreas Polymers (Basel) Article Giant vesicles (GVs) are widely-used model systems for biological membranes. The formulation of these vesicles, however, can be problematic and artifacts, such as degraded molecules or left-over oil, may be present in the final liposomes. The rapid formulation of a high number of artifact-free vesicles of uniform size using standard laboratory equipment is, therefore, highly desirable. Here, the gentle hydration method of glass bead-supported thin lipid films has been enhanced by adding a vortexing step. This led to the formulation of a uniform population of giant vesicles. Batches of glass beads coated with different lipids can be combined to produce vesicles of hybrid lipid compositions. This method represents a stable approach to rapidly generate giant vesicles. MDPI 2018-01-09 /pmc/articles/PMC6414939/ /pubmed/30966090 http://dx.doi.org/10.3390/polym10010054 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tanasescu, Radu
Mettal, Ute
Colom, Adai
Roux, Aurélien
Zumbuehl, Andreas
Facile and Rapid Formation of Giant Vesicles from Glass Beads
title Facile and Rapid Formation of Giant Vesicles from Glass Beads
title_full Facile and Rapid Formation of Giant Vesicles from Glass Beads
title_fullStr Facile and Rapid Formation of Giant Vesicles from Glass Beads
title_full_unstemmed Facile and Rapid Formation of Giant Vesicles from Glass Beads
title_short Facile and Rapid Formation of Giant Vesicles from Glass Beads
title_sort facile and rapid formation of giant vesicles from glass beads
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6414939/
https://www.ncbi.nlm.nih.gov/pubmed/30966090
http://dx.doi.org/10.3390/polym10010054
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