Cargando…
Facile and Rapid Formation of Giant Vesicles from Glass Beads
Giant vesicles (GVs) are widely-used model systems for biological membranes. The formulation of these vesicles, however, can be problematic and artifacts, such as degraded molecules or left-over oil, may be present in the final liposomes. The rapid formulation of a high number of artifact-free vesic...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6414939/ https://www.ncbi.nlm.nih.gov/pubmed/30966090 http://dx.doi.org/10.3390/polym10010054 |
_version_ | 1783403075867770880 |
---|---|
author | Tanasescu, Radu Mettal, Ute Colom, Adai Roux, Aurélien Zumbuehl, Andreas |
author_facet | Tanasescu, Radu Mettal, Ute Colom, Adai Roux, Aurélien Zumbuehl, Andreas |
author_sort | Tanasescu, Radu |
collection | PubMed |
description | Giant vesicles (GVs) are widely-used model systems for biological membranes. The formulation of these vesicles, however, can be problematic and artifacts, such as degraded molecules or left-over oil, may be present in the final liposomes. The rapid formulation of a high number of artifact-free vesicles of uniform size using standard laboratory equipment is, therefore, highly desirable. Here, the gentle hydration method of glass bead-supported thin lipid films has been enhanced by adding a vortexing step. This led to the formulation of a uniform population of giant vesicles. Batches of glass beads coated with different lipids can be combined to produce vesicles of hybrid lipid compositions. This method represents a stable approach to rapidly generate giant vesicles. |
format | Online Article Text |
id | pubmed-6414939 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64149392019-04-02 Facile and Rapid Formation of Giant Vesicles from Glass Beads Tanasescu, Radu Mettal, Ute Colom, Adai Roux, Aurélien Zumbuehl, Andreas Polymers (Basel) Article Giant vesicles (GVs) are widely-used model systems for biological membranes. The formulation of these vesicles, however, can be problematic and artifacts, such as degraded molecules or left-over oil, may be present in the final liposomes. The rapid formulation of a high number of artifact-free vesicles of uniform size using standard laboratory equipment is, therefore, highly desirable. Here, the gentle hydration method of glass bead-supported thin lipid films has been enhanced by adding a vortexing step. This led to the formulation of a uniform population of giant vesicles. Batches of glass beads coated with different lipids can be combined to produce vesicles of hybrid lipid compositions. This method represents a stable approach to rapidly generate giant vesicles. MDPI 2018-01-09 /pmc/articles/PMC6414939/ /pubmed/30966090 http://dx.doi.org/10.3390/polym10010054 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tanasescu, Radu Mettal, Ute Colom, Adai Roux, Aurélien Zumbuehl, Andreas Facile and Rapid Formation of Giant Vesicles from Glass Beads |
title | Facile and Rapid Formation of Giant Vesicles from Glass Beads |
title_full | Facile and Rapid Formation of Giant Vesicles from Glass Beads |
title_fullStr | Facile and Rapid Formation of Giant Vesicles from Glass Beads |
title_full_unstemmed | Facile and Rapid Formation of Giant Vesicles from Glass Beads |
title_short | Facile and Rapid Formation of Giant Vesicles from Glass Beads |
title_sort | facile and rapid formation of giant vesicles from glass beads |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6414939/ https://www.ncbi.nlm.nih.gov/pubmed/30966090 http://dx.doi.org/10.3390/polym10010054 |
work_keys_str_mv | AT tanasescuradu facileandrapidformationofgiantvesiclesfromglassbeads AT mettalute facileandrapidformationofgiantvesiclesfromglassbeads AT colomadai facileandrapidformationofgiantvesiclesfromglassbeads AT rouxaurelien facileandrapidformationofgiantvesiclesfromglassbeads AT zumbuehlandreas facileandrapidformationofgiantvesiclesfromglassbeads |