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Osteogenic potential of mesenchymal stem cells from rat mandible to regenerate critical sized calvarial defect
Although bone marrow–derived mesenchymal stem cells (MSCs) have been extensively explored in bone tissue engineering, only few studies using mesenchymal stem cells from mandible (M-MSCs) have been reported. However, mesenchymal stem cells from mandible have the potential to be as effective as femur-...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6415471/ https://www.ncbi.nlm.nih.gov/pubmed/30886687 http://dx.doi.org/10.1177/2041731419830427 |
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author | Lee, Dong Joon Kwon, Jane Current, Luke Yoon, Kun Zalal, Rahim Hu, Xiangxiang Xue, Peng Ko, Ching-Chang |
author_facet | Lee, Dong Joon Kwon, Jane Current, Luke Yoon, Kun Zalal, Rahim Hu, Xiangxiang Xue, Peng Ko, Ching-Chang |
author_sort | Lee, Dong Joon |
collection | PubMed |
description | Although bone marrow–derived mesenchymal stem cells (MSCs) have been extensively explored in bone tissue engineering, only few studies using mesenchymal stem cells from mandible (M-MSCs) have been reported. However, mesenchymal stem cells from mandible have the potential to be as effective as femur-derived mesenchymal stem cells (F-MSCs) in regenerating bone, especially in the orofacial regions, which share embryonic origin, proximity, and accessibility. M-MSCs were isolated and characterized using mesenchymal stem cell–specific markers, colony forming assay, and multi-potential differentiation. In vitro osteogenic potential, including proliferation, osteogenic gene expression, alkaline phosphatase activity, and mineralization, was examined and compared. Furthermore, in vivo bone formations of F-MSCs and M-MSCs in rat critical sized defect were evaluated using microCT and histology. M-MSCs from rat could be successfully isolated and expanded while preserving their MSC’s characteristics. M-MSCs demonstrated a comparable proliferation and mineralization potentials and in vivo bone formation as F-MSCs. M-MSCs is a promising cell source candidate for craniofacial bone tissue engineering. |
format | Online Article Text |
id | pubmed-6415471 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-64154712019-03-18 Osteogenic potential of mesenchymal stem cells from rat mandible to regenerate critical sized calvarial defect Lee, Dong Joon Kwon, Jane Current, Luke Yoon, Kun Zalal, Rahim Hu, Xiangxiang Xue, Peng Ko, Ching-Chang J Tissue Eng Original Article Although bone marrow–derived mesenchymal stem cells (MSCs) have been extensively explored in bone tissue engineering, only few studies using mesenchymal stem cells from mandible (M-MSCs) have been reported. However, mesenchymal stem cells from mandible have the potential to be as effective as femur-derived mesenchymal stem cells (F-MSCs) in regenerating bone, especially in the orofacial regions, which share embryonic origin, proximity, and accessibility. M-MSCs were isolated and characterized using mesenchymal stem cell–specific markers, colony forming assay, and multi-potential differentiation. In vitro osteogenic potential, including proliferation, osteogenic gene expression, alkaline phosphatase activity, and mineralization, was examined and compared. Furthermore, in vivo bone formations of F-MSCs and M-MSCs in rat critical sized defect were evaluated using microCT and histology. M-MSCs from rat could be successfully isolated and expanded while preserving their MSC’s characteristics. M-MSCs demonstrated a comparable proliferation and mineralization potentials and in vivo bone formation as F-MSCs. M-MSCs is a promising cell source candidate for craniofacial bone tissue engineering. SAGE Publications 2019-03-12 /pmc/articles/PMC6415471/ /pubmed/30886687 http://dx.doi.org/10.1177/2041731419830427 Text en © The Author(s) 2019 http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution 4.0 License (http://www.creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Article Lee, Dong Joon Kwon, Jane Current, Luke Yoon, Kun Zalal, Rahim Hu, Xiangxiang Xue, Peng Ko, Ching-Chang Osteogenic potential of mesenchymal stem cells from rat mandible to regenerate critical sized calvarial defect |
title | Osteogenic potential of mesenchymal stem cells from rat mandible to regenerate critical sized calvarial defect |
title_full | Osteogenic potential of mesenchymal stem cells from rat mandible to regenerate critical sized calvarial defect |
title_fullStr | Osteogenic potential of mesenchymal stem cells from rat mandible to regenerate critical sized calvarial defect |
title_full_unstemmed | Osteogenic potential of mesenchymal stem cells from rat mandible to regenerate critical sized calvarial defect |
title_short | Osteogenic potential of mesenchymal stem cells from rat mandible to regenerate critical sized calvarial defect |
title_sort | osteogenic potential of mesenchymal stem cells from rat mandible to regenerate critical sized calvarial defect |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6415471/ https://www.ncbi.nlm.nih.gov/pubmed/30886687 http://dx.doi.org/10.1177/2041731419830427 |
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