Cargando…
Role of CD200/CD200R Signaling Pathway in Regulation of CD4(+)T Cell Subsets During Thermal Ablation of Hepatocellular Carcinoma
BACKGROUND: In this study, we assessed the role of CD200 and CD200 receptor (CD200R) in regulating CD4(+)T cell subsets and assessed the therapeutic efficacy of thermal ablation for liver hepatocellular carcinoma (HCC) in rats. MATERIAL/METHODS: Seventy-eight male C57BL/6 rats were randomly divided...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6415591/ https://www.ncbi.nlm.nih.gov/pubmed/30838977 http://dx.doi.org/10.12659/MSM.913094 |
_version_ | 1783403216137879552 |
---|---|
author | Huang, Shengchuan Pan, Yan Zhang, Qingdong Sun, Weiping |
author_facet | Huang, Shengchuan Pan, Yan Zhang, Qingdong Sun, Weiping |
author_sort | Huang, Shengchuan |
collection | PubMed |
description | BACKGROUND: In this study, we assessed the role of CD200 and CD200 receptor (CD200R) in regulating CD4(+)T cell subsets and assessed the therapeutic efficacy of thermal ablation for liver hepatocellular carcinoma (HCC) in rats. MATERIAL/METHODS: Seventy-eight male C57BL/6 rats were randomly divided into 7 groups: a control group, a model group, a CD200FC group, an anti-CD200R1 mAb group, a thermal ablation group, a thermal ablation+CD200 FC group, and a thermal ablation+anti-CD200R1 mAb group. The levels of CD200, CD200R1, Th1, Th17, and Treg in peripheral blood were detected by flow cytometry. Immunohistochemistry was used to detect CD200, CD200R1, IFN-γ, IL-17, Foxp3 protein expression in tumor tissues. RESULTS: The levels of CD200, CD200R1, Th17, and Treg were significantly increased after CD200FC treatment (p<0.05). After treatment with anti-CD200R1 mAb, the levels of CD200, CD200R1, Th17, and Treg decreased and Th1 increased. Compared with the control group, the expression of CD200, CD200R1, IL-17, and Foxp3 in the model group increased significantly, and the expression of IFN-γ decreased significantly (p<0.05). The expression of CD200, CD200R1, IL-17, and Foxp3 was significantly reduced by adding anti-CD200R1 mAb, and the expression of IFN-γ was increased (p<0.05). After the thermal ablation treatment, the proteins continued to decrease and the expression of IFN-γ continued to increase. CONCLUSIONS: The CD200/CD200R pathway participates in HCC tumor growth and the expression of CD4(+)T cell subsets in cancer tissues. Furthermore, thermal ablation treatment inhibited cancer recurrence. |
format | Online Article Text |
id | pubmed-6415591 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-64155912019-04-17 Role of CD200/CD200R Signaling Pathway in Regulation of CD4(+)T Cell Subsets During Thermal Ablation of Hepatocellular Carcinoma Huang, Shengchuan Pan, Yan Zhang, Qingdong Sun, Weiping Med Sci Monit Animal Study BACKGROUND: In this study, we assessed the role of CD200 and CD200 receptor (CD200R) in regulating CD4(+)T cell subsets and assessed the therapeutic efficacy of thermal ablation for liver hepatocellular carcinoma (HCC) in rats. MATERIAL/METHODS: Seventy-eight male C57BL/6 rats were randomly divided into 7 groups: a control group, a model group, a CD200FC group, an anti-CD200R1 mAb group, a thermal ablation group, a thermal ablation+CD200 FC group, and a thermal ablation+anti-CD200R1 mAb group. The levels of CD200, CD200R1, Th1, Th17, and Treg in peripheral blood were detected by flow cytometry. Immunohistochemistry was used to detect CD200, CD200R1, IFN-γ, IL-17, Foxp3 protein expression in tumor tissues. RESULTS: The levels of CD200, CD200R1, Th17, and Treg were significantly increased after CD200FC treatment (p<0.05). After treatment with anti-CD200R1 mAb, the levels of CD200, CD200R1, Th17, and Treg decreased and Th1 increased. Compared with the control group, the expression of CD200, CD200R1, IL-17, and Foxp3 in the model group increased significantly, and the expression of IFN-γ decreased significantly (p<0.05). The expression of CD200, CD200R1, IL-17, and Foxp3 was significantly reduced by adding anti-CD200R1 mAb, and the expression of IFN-γ was increased (p<0.05). After the thermal ablation treatment, the proteins continued to decrease and the expression of IFN-γ continued to increase. CONCLUSIONS: The CD200/CD200R pathway participates in HCC tumor growth and the expression of CD4(+)T cell subsets in cancer tissues. Furthermore, thermal ablation treatment inhibited cancer recurrence. International Scientific Literature, Inc. 2019-03-06 /pmc/articles/PMC6415591/ /pubmed/30838977 http://dx.doi.org/10.12659/MSM.913094 Text en © Med Sci Monit, 2019 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) ) |
spellingShingle | Animal Study Huang, Shengchuan Pan, Yan Zhang, Qingdong Sun, Weiping Role of CD200/CD200R Signaling Pathway in Regulation of CD4(+)T Cell Subsets During Thermal Ablation of Hepatocellular Carcinoma |
title | Role of CD200/CD200R Signaling Pathway in Regulation of CD4(+)T Cell Subsets During Thermal Ablation of Hepatocellular Carcinoma |
title_full | Role of CD200/CD200R Signaling Pathway in Regulation of CD4(+)T Cell Subsets During Thermal Ablation of Hepatocellular Carcinoma |
title_fullStr | Role of CD200/CD200R Signaling Pathway in Regulation of CD4(+)T Cell Subsets During Thermal Ablation of Hepatocellular Carcinoma |
title_full_unstemmed | Role of CD200/CD200R Signaling Pathway in Regulation of CD4(+)T Cell Subsets During Thermal Ablation of Hepatocellular Carcinoma |
title_short | Role of CD200/CD200R Signaling Pathway in Regulation of CD4(+)T Cell Subsets During Thermal Ablation of Hepatocellular Carcinoma |
title_sort | role of cd200/cd200r signaling pathway in regulation of cd4(+)t cell subsets during thermal ablation of hepatocellular carcinoma |
topic | Animal Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6415591/ https://www.ncbi.nlm.nih.gov/pubmed/30838977 http://dx.doi.org/10.12659/MSM.913094 |
work_keys_str_mv | AT huangshengchuan roleofcd200cd200rsignalingpathwayinregulationofcd4tcellsubsetsduringthermalablationofhepatocellularcarcinoma AT panyan roleofcd200cd200rsignalingpathwayinregulationofcd4tcellsubsetsduringthermalablationofhepatocellularcarcinoma AT zhangqingdong roleofcd200cd200rsignalingpathwayinregulationofcd4tcellsubsetsduringthermalablationofhepatocellularcarcinoma AT sunweiping roleofcd200cd200rsignalingpathwayinregulationofcd4tcellsubsetsduringthermalablationofhepatocellularcarcinoma |