Cargando…
Differential prevalence and geographic distribution of hepatitis C virus genotypes in acute and chronic hepatitis C patients in Vietnam
BACKGROUND: The highest burden of disease from hepatitis C virus (HCV) is found in Southeast Asia, but our understanding of the epidemiology of infection in many heavily burdened countries is still limited. In particular, there is relatively little data on acute HCV infection, the outcome of which c...
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6415813/ https://www.ncbi.nlm.nih.gov/pubmed/30865664 http://dx.doi.org/10.1371/journal.pone.0212734 |
_version_ | 1783403238796558336 |
---|---|
author | Le Ngoc, Chau Tran Thi Thanh, Thanh Tran Thi Lan, Phuong Nguyen Mai, Trinh Nguyen Hoa, Trang Nghiem My, Ngoc Le Van, Tan Le Manh, Hung Le Thanh, Phuong Nguyen Van Vinh, Chau Thwaites, Guy Cooke, Graham Heilek, Gabrielle M. Shikuma, Cecilia Le, Thuy Baker, Stephen Rahman, Motiur |
author_facet | Le Ngoc, Chau Tran Thi Thanh, Thanh Tran Thi Lan, Phuong Nguyen Mai, Trinh Nguyen Hoa, Trang Nghiem My, Ngoc Le Van, Tan Le Manh, Hung Le Thanh, Phuong Nguyen Van Vinh, Chau Thwaites, Guy Cooke, Graham Heilek, Gabrielle M. Shikuma, Cecilia Le, Thuy Baker, Stephen Rahman, Motiur |
author_sort | Le Ngoc, Chau |
collection | PubMed |
description | BACKGROUND: The highest burden of disease from hepatitis C virus (HCV) is found in Southeast Asia, but our understanding of the epidemiology of infection in many heavily burdened countries is still limited. In particular, there is relatively little data on acute HCV infection, the outcome of which can be influenced by both viral and host genetics which differ within the region. We studied HCV genotype and IL28B gene polymorphism in a cohort of acute HCV-infected patients in Southern Vietnam alongside two other cohorts of chronic HCV-infected patients to better understand the epidemiology of HCV infection locally and inform the development of programs for therapy with the increasing availability of directly acting antiviral therapy (DAAs). METHODS: We analysed plasma samples from patients with acute and chronic HCV infection, including chronic HCV mono-infection and chronic Human Immunodeficiency Virus (HIV)-HCV coinfection, who enrolled in four epidemiological or clinical research studies. HCV infection was confirmed with RNA testing. The 5’ UTR, core and NSB5 regions of HCV RNA positive samples were sequenced, and the genotype and subtype of the viral strains were determined. Host DNA from all HCV positive patients and age- and sex-matched non-HCV-infected control individuals were analysed for IL28B single nucleotide polymorphism (SNP) (rs12979860 and rs8099917). Geolocation of the patients were mapped using QGIS. RESULTS: 355 HCV antibody positive patients were analysed; 54.6% (194/355) and 46.4% (161/355) were acute and chronic infections, respectively. 50.4% (81/161) and 49.6.4% (80/161) of chronic infections had HCV mono-infection and HIV-HCV coinfection, respectively. 88.7% (315/355) and 10.1% (36/355) of the patients were from southern and central regions of Vietnam, respectively. 92.4% (328/355) of patients were HCV RNA positive, including 86.1% (167/194) acute and 100% (161/161) chronic infections. Genotype could be determined in 98.4% (322/328) patients. Genotypes 1 (56.5%; 182/322) and 6 (33.9%; 109/322) predominated. Genotype 1 including genotype 1a was significantly higher in HIV-HCV coinfected patients compared to acute HCV patients [43.8% (35/80) versus 20.5% (33/167)], (p = <0.001), while genotype 6 was significantly higher in chronic HCV mono-infected patients [(44.4% (36/81) versus 20.0% (16/80)] (p = < 0.004) compared to HIV-HCV coinfected patients. The prevalence of IL28B SNP (rs12979860) homozygous CC was 86.46% (83/96) in control individuals and was significantly higher in acutely-infected compared to chronically-infected patients [93.2 (82/88) versus 76.1% (35/46)] (p = < 0.005). CONCLUSION: HCV genotype 6 is highly prevalent in Vietnam and the high prevalence in treatment naïve chronic HCV patients may results from poor spontaneous clearance of acute HCV infection with genotype 6. |
format | Online Article Text |
id | pubmed-6415813 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-64158132019-04-02 Differential prevalence and geographic distribution of hepatitis C virus genotypes in acute and chronic hepatitis C patients in Vietnam Le Ngoc, Chau Tran Thi Thanh, Thanh Tran Thi Lan, Phuong Nguyen Mai, Trinh Nguyen Hoa, Trang Nghiem My, Ngoc Le Van, Tan Le Manh, Hung Le Thanh, Phuong Nguyen Van Vinh, Chau Thwaites, Guy Cooke, Graham Heilek, Gabrielle M. Shikuma, Cecilia Le, Thuy Baker, Stephen Rahman, Motiur PLoS One Research Article BACKGROUND: The highest burden of disease from hepatitis C virus (HCV) is found in Southeast Asia, but our understanding of the epidemiology of infection in many heavily burdened countries is still limited. In particular, there is relatively little data on acute HCV infection, the outcome of which can be influenced by both viral and host genetics which differ within the region. We studied HCV genotype and IL28B gene polymorphism in a cohort of acute HCV-infected patients in Southern Vietnam alongside two other cohorts of chronic HCV-infected patients to better understand the epidemiology of HCV infection locally and inform the development of programs for therapy with the increasing availability of directly acting antiviral therapy (DAAs). METHODS: We analysed plasma samples from patients with acute and chronic HCV infection, including chronic HCV mono-infection and chronic Human Immunodeficiency Virus (HIV)-HCV coinfection, who enrolled in four epidemiological or clinical research studies. HCV infection was confirmed with RNA testing. The 5’ UTR, core and NSB5 regions of HCV RNA positive samples were sequenced, and the genotype and subtype of the viral strains were determined. Host DNA from all HCV positive patients and age- and sex-matched non-HCV-infected control individuals were analysed for IL28B single nucleotide polymorphism (SNP) (rs12979860 and rs8099917). Geolocation of the patients were mapped using QGIS. RESULTS: 355 HCV antibody positive patients were analysed; 54.6% (194/355) and 46.4% (161/355) were acute and chronic infections, respectively. 50.4% (81/161) and 49.6.4% (80/161) of chronic infections had HCV mono-infection and HIV-HCV coinfection, respectively. 88.7% (315/355) and 10.1% (36/355) of the patients were from southern and central regions of Vietnam, respectively. 92.4% (328/355) of patients were HCV RNA positive, including 86.1% (167/194) acute and 100% (161/161) chronic infections. Genotype could be determined in 98.4% (322/328) patients. Genotypes 1 (56.5%; 182/322) and 6 (33.9%; 109/322) predominated. Genotype 1 including genotype 1a was significantly higher in HIV-HCV coinfected patients compared to acute HCV patients [43.8% (35/80) versus 20.5% (33/167)], (p = <0.001), while genotype 6 was significantly higher in chronic HCV mono-infected patients [(44.4% (36/81) versus 20.0% (16/80)] (p = < 0.004) compared to HIV-HCV coinfected patients. The prevalence of IL28B SNP (rs12979860) homozygous CC was 86.46% (83/96) in control individuals and was significantly higher in acutely-infected compared to chronically-infected patients [93.2 (82/88) versus 76.1% (35/46)] (p = < 0.005). CONCLUSION: HCV genotype 6 is highly prevalent in Vietnam and the high prevalence in treatment naïve chronic HCV patients may results from poor spontaneous clearance of acute HCV infection with genotype 6. Public Library of Science 2019-03-13 /pmc/articles/PMC6415813/ /pubmed/30865664 http://dx.doi.org/10.1371/journal.pone.0212734 Text en © 2019 Le Ngoc et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Le Ngoc, Chau Tran Thi Thanh, Thanh Tran Thi Lan, Phuong Nguyen Mai, Trinh Nguyen Hoa, Trang Nghiem My, Ngoc Le Van, Tan Le Manh, Hung Le Thanh, Phuong Nguyen Van Vinh, Chau Thwaites, Guy Cooke, Graham Heilek, Gabrielle M. Shikuma, Cecilia Le, Thuy Baker, Stephen Rahman, Motiur Differential prevalence and geographic distribution of hepatitis C virus genotypes in acute and chronic hepatitis C patients in Vietnam |
title | Differential prevalence and geographic distribution of hepatitis C virus genotypes in acute and chronic hepatitis C patients in Vietnam |
title_full | Differential prevalence and geographic distribution of hepatitis C virus genotypes in acute and chronic hepatitis C patients in Vietnam |
title_fullStr | Differential prevalence and geographic distribution of hepatitis C virus genotypes in acute and chronic hepatitis C patients in Vietnam |
title_full_unstemmed | Differential prevalence and geographic distribution of hepatitis C virus genotypes in acute and chronic hepatitis C patients in Vietnam |
title_short | Differential prevalence and geographic distribution of hepatitis C virus genotypes in acute and chronic hepatitis C patients in Vietnam |
title_sort | differential prevalence and geographic distribution of hepatitis c virus genotypes in acute and chronic hepatitis c patients in vietnam |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6415813/ https://www.ncbi.nlm.nih.gov/pubmed/30865664 http://dx.doi.org/10.1371/journal.pone.0212734 |
work_keys_str_mv | AT lengocchau differentialprevalenceandgeographicdistributionofhepatitiscvirusgenotypesinacuteandchronichepatitiscpatientsinvietnam AT tranthithanhthanh differentialprevalenceandgeographicdistributionofhepatitiscvirusgenotypesinacuteandchronichepatitiscpatientsinvietnam AT tranthilanphuong differentialprevalenceandgeographicdistributionofhepatitiscvirusgenotypesinacuteandchronichepatitiscpatientsinvietnam AT nguyenmaitrinh differentialprevalenceandgeographicdistributionofhepatitiscvirusgenotypesinacuteandchronichepatitiscpatientsinvietnam AT nguyenhoatrang differentialprevalenceandgeographicdistributionofhepatitiscvirusgenotypesinacuteandchronichepatitiscpatientsinvietnam AT nghiemmyngoc differentialprevalenceandgeographicdistributionofhepatitiscvirusgenotypesinacuteandchronichepatitiscpatientsinvietnam AT levantan differentialprevalenceandgeographicdistributionofhepatitiscvirusgenotypesinacuteandchronichepatitiscpatientsinvietnam AT lemanhhung differentialprevalenceandgeographicdistributionofhepatitiscvirusgenotypesinacuteandchronichepatitiscpatientsinvietnam AT lethanhphuong differentialprevalenceandgeographicdistributionofhepatitiscvirusgenotypesinacuteandchronichepatitiscpatientsinvietnam AT nguyenvanvinhchau differentialprevalenceandgeographicdistributionofhepatitiscvirusgenotypesinacuteandchronichepatitiscpatientsinvietnam AT thwaitesguy differentialprevalenceandgeographicdistributionofhepatitiscvirusgenotypesinacuteandchronichepatitiscpatientsinvietnam AT cookegraham differentialprevalenceandgeographicdistributionofhepatitiscvirusgenotypesinacuteandchronichepatitiscpatientsinvietnam AT heilekgabriellem differentialprevalenceandgeographicdistributionofhepatitiscvirusgenotypesinacuteandchronichepatitiscpatientsinvietnam AT shikumacecilia differentialprevalenceandgeographicdistributionofhepatitiscvirusgenotypesinacuteandchronichepatitiscpatientsinvietnam AT lethuy differentialprevalenceandgeographicdistributionofhepatitiscvirusgenotypesinacuteandchronichepatitiscpatientsinvietnam AT bakerstephen differentialprevalenceandgeographicdistributionofhepatitiscvirusgenotypesinacuteandchronichepatitiscpatientsinvietnam AT rahmanmotiur differentialprevalenceandgeographicdistributionofhepatitiscvirusgenotypesinacuteandchronichepatitiscpatientsinvietnam AT differentialprevalenceandgeographicdistributionofhepatitiscvirusgenotypesinacuteandchronichepatitiscpatientsinvietnam |