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Live cell monitoring of double strand breaks in S. cerevisiae

We have used two different live-cell fluorescent protein markers to monitor the formation and localization of double-strand breaks (DSBs) in budding yeast. Using GFP derivatives of the Rad51 recombination protein or the Ddc2 checkpoint protein, we find that cells with three site-specific DSBs, on di...

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Detalles Bibliográficos
Autores principales:	Waterman, David P., Zhou, Felix, Li, Kevin, Lee, Cheng-Sheng, Tsabar, Michael, Eapen, Vinay V., Mazzella, Allison, Haber, James E.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2019
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6415866/ https://www.ncbi.nlm.nih.gov/pubmed/30822309 http://dx.doi.org/10.1371/journal.pgen.1008001

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