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Stress response to CO(2) deprivation by Arabidopsis thaliana in plant cultures

After being the standard plant propagation protocol for decades, cultures of Arabidopsis thaliana sealed with Parafilm remain common today out of practicality, habit, or necessity (as in co-cultures with microorganisms). Regardless of concerns over the aeration of these cultures, no investigation ha...

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Autores principales: Banerjee, Souvik, Siemianowski, Oskar, Liu, Meiling, Lind, Kara R., Tian, Xinchun, Nettleton, Dan, Cademartiri, Ludovico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6415875/
https://www.ncbi.nlm.nih.gov/pubmed/30865661
http://dx.doi.org/10.1371/journal.pone.0212462
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author Banerjee, Souvik
Siemianowski, Oskar
Liu, Meiling
Lind, Kara R.
Tian, Xinchun
Nettleton, Dan
Cademartiri, Ludovico
author_facet Banerjee, Souvik
Siemianowski, Oskar
Liu, Meiling
Lind, Kara R.
Tian, Xinchun
Nettleton, Dan
Cademartiri, Ludovico
author_sort Banerjee, Souvik
collection PubMed
description After being the standard plant propagation protocol for decades, cultures of Arabidopsis thaliana sealed with Parafilm remain common today out of practicality, habit, or necessity (as in co-cultures with microorganisms). Regardless of concerns over the aeration of these cultures, no investigation has explored the CO(2) transport inside these cultures and its effect on the plants. Thereby, it was impossible to assess whether Parafilm-seals used today or in thousands of older papers in the literature constitute a treatment, and whether this treatment could potentially affect the study of other treatments.For the first time we report the CO(2) concentrations in Parafilm-sealed cultures of A. thaliana with a 1 minute temporal resolution, and the transcriptome comparison with aerated cultures. The data show significant CO(2) deprivation to the plants, a drastic suppression of photosynthesis, respiration, starch accumulation, chlorophyll biosynthesis, and an increased accumulation of reactive oxygen species. Most importantly, CO(2) deprivation occurs as soon as the cotyledons emerge. Gene expression analysis indicates a significant alteration of 35% of the pathways when compared to aerated cultures, especially in stress response and secondary metabolism processes. On the other hand, the observed increase in the production of glucosinolates and flavonoids suggests intriguing possibilities for CO(2) deprivation as an organic biofortification treatment in high-value crops.
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spelling pubmed-64158752019-04-02 Stress response to CO(2) deprivation by Arabidopsis thaliana in plant cultures Banerjee, Souvik Siemianowski, Oskar Liu, Meiling Lind, Kara R. Tian, Xinchun Nettleton, Dan Cademartiri, Ludovico PLoS One Research Article After being the standard plant propagation protocol for decades, cultures of Arabidopsis thaliana sealed with Parafilm remain common today out of practicality, habit, or necessity (as in co-cultures with microorganisms). Regardless of concerns over the aeration of these cultures, no investigation has explored the CO(2) transport inside these cultures and its effect on the plants. Thereby, it was impossible to assess whether Parafilm-seals used today or in thousands of older papers in the literature constitute a treatment, and whether this treatment could potentially affect the study of other treatments.For the first time we report the CO(2) concentrations in Parafilm-sealed cultures of A. thaliana with a 1 minute temporal resolution, and the transcriptome comparison with aerated cultures. The data show significant CO(2) deprivation to the plants, a drastic suppression of photosynthesis, respiration, starch accumulation, chlorophyll biosynthesis, and an increased accumulation of reactive oxygen species. Most importantly, CO(2) deprivation occurs as soon as the cotyledons emerge. Gene expression analysis indicates a significant alteration of 35% of the pathways when compared to aerated cultures, especially in stress response and secondary metabolism processes. On the other hand, the observed increase in the production of glucosinolates and flavonoids suggests intriguing possibilities for CO(2) deprivation as an organic biofortification treatment in high-value crops. Public Library of Science 2019-03-13 /pmc/articles/PMC6415875/ /pubmed/30865661 http://dx.doi.org/10.1371/journal.pone.0212462 Text en © 2019 Banerjee et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Banerjee, Souvik
Siemianowski, Oskar
Liu, Meiling
Lind, Kara R.
Tian, Xinchun
Nettleton, Dan
Cademartiri, Ludovico
Stress response to CO(2) deprivation by Arabidopsis thaliana in plant cultures
title Stress response to CO(2) deprivation by Arabidopsis thaliana in plant cultures
title_full Stress response to CO(2) deprivation by Arabidopsis thaliana in plant cultures
title_fullStr Stress response to CO(2) deprivation by Arabidopsis thaliana in plant cultures
title_full_unstemmed Stress response to CO(2) deprivation by Arabidopsis thaliana in plant cultures
title_short Stress response to CO(2) deprivation by Arabidopsis thaliana in plant cultures
title_sort stress response to co(2) deprivation by arabidopsis thaliana in plant cultures
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6415875/
https://www.ncbi.nlm.nih.gov/pubmed/30865661
http://dx.doi.org/10.1371/journal.pone.0212462
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